(data stored in SCRATCH zone)

SWISSPROT: D3VH67_XENNA

ID   D3VH67_XENNA            Unreviewed;       295 AA.
AC   D3VH67;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   08-MAY-2019, entry version 58.
DE   RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
DE            EC=1.5.1.20 {ECO:0000256|RuleBase:RU003862};
GN   Name=metF {ECO:0000313|EMBL:CBJ88352.1};
GN   OrderedLocusNames=XNC1_0264 {ECO:0000313|EMBL:CBJ88352.1};
OS   Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 /
OS   NCIB 9965 / AN6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Xenorhabdus.
OX   NCBI_TaxID=406817 {ECO:0000313|EMBL:CBJ88352.1, ECO:0000313|Proteomes:UP000008075};
RN   [1] {ECO:0000313|Proteomes:UP000008075}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6
RC   {ECO:0000313|Proteomes:UP000008075};
RA   Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S.,
RA   Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P.,
RA   Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S.,
RA   Suen G.;
RT   "Complete genome sequence of Xenorhabdus nematophila (strain ATCC
RT   19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6).";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBJ88352.1, ECO:0000313|Proteomes:UP000008075}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6
RC   {ECO:0000313|Proteomes:UP000008075};
RX   PubMed=22125637; DOI=10.1371/journal.pone.0027909;
RA   Chaston J.M., Suen G., Tucker S.L., Andersen A.W., Bhasin A., Bode E.,
RA   Bode H.B., Brachmann A.O., Cowles C.E., Cowles K.N., Darby C.,
RA   de Leon L., Drace K., Du Z., Givaudan A., Herbert Tran E.E.,
RA   Jewell K.A., Knack J.J., Krasomil-Osterfeld K.C., Kukor R., Lanois A.,
RA   Latreille P., Leimgruber N.K., Lipke C.M., Liu R., Lu X.,
RA   Martens E.C., Marri P.R., Medigue C., Menard M.L., Miller N.M.,
RA   Morales-Soto N., Norton S., Ogier J.C., Orchard S.S., Park D.,
RA   Park Y., Qurollo B.A., Sugar D.R., Richards G.R., Rouy Z.,
RA   Slominski B., Slominski K., Snyder H., Tjaden B.C., van der Hoeven R.,
RA   Welch R.D., Wheeler C., Xiang B., Barbazuk B., Gaudriault S.,
RA   Goodner B., Slater S.C., Forst S., Goldman B.S., Goodrich-Blair H.;
RT   "The entomopathogenic bacterial endosymbionts xenorhabdus and
RT   photorhabdus: convergent lifestyles from divergent genomes.";
RL   PLoS ONE 6:e27909-e27909(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-
CC         5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC         Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.5.1.20; Evidence={ECO:0000256|RuleBase:RU003862};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|RuleBase:RU003862};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|RuleBase:RU003862}.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family. {ECO:0000256|RuleBase:RU003862}.
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DR   EMBL; FN667742; CBJ88352.1; -; Genomic_DNA.
DR   RefSeq; WP_013183202.1; NC_014228.1.
DR   STRING; 406817.XNC1_0264; -.
DR   EnsemblBacteria; CBJ88352; CBJ88352; XNC1_0264.
DR   KEGG; xne:XNC1_0264; -.
DR   eggNOG; ENOG4105SYT; Bacteria.
DR   eggNOG; COG0685; LUCA.
DR   HOGENOM; HOG000246232; -.
DR   KO; K00297; -.
DR   OMA; FIRAETG; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000008075; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00537; MTHFR; 1.
DR   InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   InterPro; IPR004620; MTHF_reductase_bac.
DR   Pfam; PF02219; MTHFR; 1.
DR   SUPFAM; SSF51730; SSF51730; 1.
DR   TIGRFAMs; TIGR00676; fadh2; 1.
PE   3: Inferred from homology;
DR   PRODOM; D3VH67.
DR   SWISS-2DPAGE; D3VH67.
KW   Amino-acid biosynthesis {ECO:0000256|RuleBase:RU004255};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008075};
KW   FAD {ECO:0000256|RuleBase:RU003862};
KW   Flavoprotein {ECO:0000256|RuleBase:RU003862};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU003862,
KW   ECO:0000313|EMBL:CBJ88352.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008075}.
SQ   SEQUENCE   295 AA;  33513 MW;  8F29B6DCC0CDAF20 CRC64;
     MSFFHANQRE ALNQNLAELE GQIRVSFEFF PPSTTEMEQT LWQSIDRLSR LKPKFVSVTY
     GANSGERNRT HSIIKGIKEK TGLDAAPHLT CIDASREELR DIAHDYWQSG IRHIVALRGD
     LPNNCCKPEM YGADLVELLK EEADFDISVA AYPEVHPEAK SAQSDLINLK HKIDAGANRA
     ITQFFFDVES YLRFRDRCVA TGIDVEIVPG ILPVSNFRQL QRFSTMTNVR IPSWMTRMFE
     GLDDDPESRN LVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPV
//

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