(data stored in SCRATCH zone)

SWISSPROT: D4YWY2_SPHJU

ID   D4YWY2_SPHJU            Unreviewed;       427 AA.
AC   D4YWY2;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   07-JUN-2017, entry version 58.
DE   RecName: Full=tRNA modification GTPase MnmE {ECO:0000256|HAMAP-Rule:MF_00379};
DE            EC=3.6.-.- {ECO:0000256|HAMAP-Rule:MF_00379};
GN   Name=thdF {ECO:0000313|EMBL:BAI94864.1};
GN   Synonyms=mnmE {ECO:0000256|HAMAP-Rule:MF_00379}, trmE
GN   {ECO:0000256|HAMAP-Rule:MF_00379};
GN   OrderedLocusNames=SJA_C1-00300 {ECO:0000313|EMBL:BAI94864.1};
OS   Sphingobium japonicum (strain NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662 {ECO:0000313|EMBL:BAI94864.1, ECO:0000313|Proteomes:UP000007753};
RN   [1] {ECO:0000313|EMBL:BAI94864.1, ECO:0000313|Proteomes:UP000007753}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101211 / UT26S {ECO:0000313|Proteomes:UP000007753};
RX   PubMed=20817768; DOI=10.1128/JB.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A.,
RA   Fukui S., Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum
RT   UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate.
CC       Involved in the addition of a carboxymethylaminomethyl (cmnm)
CC       group at the wobble position (U34) of certain tRNAs, forming tRNA-
CC       cmnm(5)s(2)U34. {ECO:0000256|HAMAP-Rule:MF_00379}.
CC   -!- COFACTOR:
CC       Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00379};
CC       Note=Binds 1 potassium ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00379};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG
CC       subunits. {ECO:0000256|HAMAP-Rule:MF_00379}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00379}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-
CC       like GTPase superfamily. TrmE GTPase family. {ECO:0000256|HAMAP-
CC       Rule:MF_00379, ECO:0000256|SAAS:SAAS00629561}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00379}.
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DR   EMBL; AP010803; BAI94864.1; -; Genomic_DNA.
DR   RefSeq; WP_013038710.1; NC_014006.1.
DR   ProteinModelPortal; D4YWY2; -.
DR   STRING; 452662.SJA_C1-00300; -.
DR   EnsemblBacteria; BAI94864; BAI94864; SJA_C1-00300.
DR   GeneID; 29271740; -.
DR   KEGG; sjp:SJA_C1-00300; -.
DR   eggNOG; ENOG4105C1H; Bacteria.
DR   eggNOG; COG0486; LUCA.
DR   HOGENOM; HOG000200714; -.
DR   KO; K03650; -.
DR   OMA; CEIQCHG; -.
DR   OrthoDB; POG091H01GD; -.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:UniProtKB-HAMAP.
DR   CDD; cd04164; trmE; 1.
DR   HAMAP; MF_00379; GTPase_MnmE; 1.
DR   InterPro; IPR031168; G_TrmE.
DR   InterPro; IPR018948; GTP-bd_TrmE_N.
DR   InterPro; IPR006073; GTP_binding_domain.
DR   InterPro; IPR004520; GTPase_MnmE.
DR   InterPro; IPR025867; MnmE_helical.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   Pfam; PF12631; MnmE_helical; 1.
DR   Pfam; PF10396; TrmE_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51709; G_TRME; 1.
PE   3: Inferred from homology;
DR   PRODOM; D4YWY2.
DR   SWISS-2DPAGE; D4YWY2.
KW   Complete proteome {ECO:0000313|Proteomes:UP000007753};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00379};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00379,
KW   ECO:0000256|SAAS:SAAS00629560};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00379};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00379};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00379};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00379,
KW   ECO:0000256|SAAS:SAAS00629545};
KW   Potassium {ECO:0000256|HAMAP-Rule:MF_00379};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007753};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00379,
KW   ECO:0000256|SAAS:SAAS00629563}.
FT   DOMAIN      216    354       TrmE-type G (guanine nucleotide-binding).
FT                                {ECO:0000259|PROSITE:PS51709}.
FT   NP_BIND     226    231       GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   NP_BIND     245    251       GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   NP_BIND     270    273       GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   METAL       226    226       Potassium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00379}.
FT   METAL       230    230       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00379}.
FT   METAL       245    245       Potassium; via carbonyl oxygen.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   METAL       247    247       Potassium; via carbonyl oxygen.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   METAL       250    250       Potassium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00379}.
FT   METAL       251    251       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00379}.
FT   BINDING      21     21       Formyltetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   BINDING      79     79       Formyltetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   BINDING     119    119       Formyltetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
FT   BINDING     427    427       Formyltetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00379}.
SQ   SEQUENCE   427 AA;  45090 MW;  849B63A477317931 CRC64;
     MTETIFALSS GAPPAGIAVV RISGPGAGQA LLALAGSLPP ARRARLSSLR DPRDGALLDR
     ALVLWFPGPD TATGEDLAEL HCHGGRAVIA AVEQALASLQ GLRRAEAGEF TRRAFANGRM
     DLNAVEGLSD LLAAETQQQR RAALLMAEGH FSQRVEQWLR TLLDLSAMTE AALDFSDEDD
     VPDHGIETRI GQGVRILAED VRAVLASPSA ERLRDGVRVV LAGPPNAGKS TLLNRLVGRD
     AAIVSDVAGT TRDRIEVPAA LGGTAFLFTD TAGLRGETGD AIEAIGIDRA RGALEAADII
     LWLGPADEAP REDAIVIAAQ CDRHAKADRP GLPLSAVTGE GMDRLASMLL ERAAMLLPGE
     GDYALHQRQR EGAARLHAHL TSAQGSDDLL IVAEELRQAR HAIDLLTGRA GTEDMLDRLF
     AGFCIGK
//

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