(data stored in SCRATCH zone)

SWISSPROT: D4YXR8_SPHJU

ID   D4YXR8_SPHJU            Unreviewed;       308 AA.
AC   D4YXR8;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   07-JUN-2017, entry version 51.
DE   RecName: Full=Porphobilinogen deaminase {ECO:0000256|HAMAP-Rule:MF_00260};
DE            Short=PBG {ECO:0000256|HAMAP-Rule:MF_00260};
DE            EC=2.5.1.61 {ECO:0000256|HAMAP-Rule:MF_00260};
DE   AltName: Full=Hydroxymethylbilane synthase {ECO:0000256|HAMAP-Rule:MF_00260};
DE            Short=HMBS {ECO:0000256|HAMAP-Rule:MF_00260};
DE   AltName: Full=Pre-uroporphyrinogen synthase {ECO:0000256|HAMAP-Rule:MF_00260};
GN   Name=hemC {ECO:0000256|HAMAP-Rule:MF_00260,
GN   ECO:0000313|EMBL:BAI95150.1};
GN   OrderedLocusNames=SJA_C1-03160 {ECO:0000313|EMBL:BAI95150.1};
OS   Sphingobium japonicum (strain NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662 {ECO:0000313|EMBL:BAI95150.1, ECO:0000313|Proteomes:UP000007753};
RN   [1] {ECO:0000313|EMBL:BAI95150.1, ECO:0000313|Proteomes:UP000007753}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101211 / UT26S {ECO:0000313|Proteomes:UP000007753};
RX   PubMed=20817768; DOI=10.1128/JB.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A.,
RA   Fukui S., Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum
RT   UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete
CC       steps. {ECO:0000256|HAMAP-Rule:MF_00260,
CC       ECO:0000256|SAAS:SAAS00347445}.
CC   -!- CATALYTIC ACTIVITY: 4 porphobilinogen + H(2)O =
CC       hydroxymethylbilane + 4 NH(3). {ECO:0000256|HAMAP-Rule:MF_00260,
CC       ECO:0000256|SAAS:SAAS00347467}.
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00260};
CC       Note=Binds 1 dipyrromethane group covalently. {ECO:0000256|HAMAP-
CC       Rule:MF_00260};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-
CC       IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate:
CC       step 2/4. {ECO:0000256|HAMAP-Rule:MF_00260}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00260}.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000256|HAMAP-Rule:MF_00260}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000256|HAMAP-
CC       Rule:MF_00260, ECO:0000256|SAAS:SAAS00542451}.
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DR   EMBL; AP010803; BAI95150.1; -; Genomic_DNA.
DR   RefSeq; WP_013038938.1; NC_014006.1.
DR   STRING; 452662.SJA_C1-03160; -.
DR   EnsemblBacteria; BAI95150; BAI95150; SJA_C1-03160.
DR   GeneID; 29272018; -.
DR   KEGG; sjp:SJA_C1-03160; -.
DR   eggNOG; ENOG4105D6W; Bacteria.
DR   eggNOG; COG0181; LUCA.
DR   HOGENOM; HOG000228588; -.
DR   KO; K01749; -.
DR   OMA; CQVPIGV; -.
DR   OrthoDB; POG091H064B; -.
DR   UniPathway; UPA00251; UER00319.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   HAMAP; MF_00260; Porphobil_deam; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 1.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
PE   3: Inferred from homology;
DR   PRODOM; D4YXR8.
DR   SWISS-2DPAGE; D4YXR8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000007753};
KW   Porphyrin biosynthesis {ECO:0000256|HAMAP-Rule:MF_00260,
KW   ECO:0000256|SAAS:SAAS00018392};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007753};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00260,
KW   ECO:0000256|SAAS:SAAS00018489, ECO:0000313|EMBL:BAI95150.1}.
FT   DOMAIN        8    213       Porphobil_deam. {ECO:0000259|Pfam:
FT                                PF01379}.
FT   DOMAIN      228    294       Porphobil_deamC. {ECO:0000259|Pfam:
FT                                PF03900}.
FT   MOD_RES     243    243       S-(dipyrrolylmethanemethyl)cysteine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00260}.
SQ   SEQUENCE   308 AA;  32585 MW;  157EC5D2BCEFE603 CRC64;
     MQKPSRPLRL GTRGSPLALA QAHMTVAALR ARHGWDEGAV EIVVVQTSGD RIQDRALADI
     GGKALWTKEL DRALASGEID FAVHSMKDVE TIRPAEIRIA AMLPRADVRD RLVGADDFAA
     LPERPVVGTS SPRRAAQVRR LRPDAQVVLF RGNVATRLAR LDRGEAHATL LAAAGLDRLG
     QGDVGVAIPV DVMLPAPSQG AVGIETLSGD EAMLDLLAAI SDADTFDCVM AERAVLAGLG
     GTCHSPIAAF ARLEDGQVRL RAEIISPDGR ETVGETARIE RGDAAAAEAM GRGLLDRASP
     ALRLLFEA
//

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