(data stored in SCRATCH zone)

SWISSPROT: D5E8V9_METMS

ID   D5E8V9_METMS            Unreviewed;       345 AA.
AC   D5E8V9;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   11-DEC-2019, entry version 53.
DE   RecName: Full=GDP-mannose 4,6-dehydratase {ECO:0000256|HAMAP-Rule:MF_00955};
DE            EC=4.2.1.47 {ECO:0000256|HAMAP-Rule:MF_00955};
DE   AltName: Full=GDP-D-mannose dehydratase {ECO:0000256|HAMAP-Rule:MF_00955};
GN   Name=gmd {ECO:0000256|HAMAP-Rule:MF_00955};
GN   OrderedLocusNames=Mmah_0082 {ECO:0000313|EMBL:ADE35618.1};
OS   Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanohalophilus.
OX   NCBI_TaxID=547558 {ECO:0000313|EMBL:ADE35618.1, ECO:0000313|Proteomes:UP000001059};
RN   [1] {ECO:0000313|EMBL:ADE35618.1, ECO:0000313|Proteomes:UP000001059}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35705 / DSM 5219 / SLP
RC   {ECO:0000313|Proteomes:UP000001059};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA   Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M.,
RA   Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D.,
RA   Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.;
RT   "The complete genome of Methanohalophilus mahii DSM 5219.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-
CC       deoxy-D-mannose. {ECO:0000256|HAMAP-Rule:MF_00955}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O;
CC         Xref=Rhea:RHEA:23820, ChEBI:CHEBI:15377, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:57964; EC=4.2.1.47; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00955};
CC   -!- COFACTOR:
CC       Name=NADP(+); Xref=ChEBI:CHEBI:58349;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00955};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. GDP-mannose 4,6-dehydratase subfamily. {ECO:0000256|HAMAP-
CC       Rule:MF_00955}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|HAMAP-Rule:MF_00955}.
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DR   EMBL; CP001994; ADE35618.1; -; Genomic_DNA.
DR   RefSeq; WP_013036561.1; NC_014002.1.
DR   EnsemblBacteria; ADE35618; ADE35618; Mmah_0082.
DR   GeneID; 8982213; -.
DR   KEGG; mmh:Mmah_0082; -.
DR   eggNOG; arCOG01373; Archaea.
DR   eggNOG; COG1089; LUCA.
DR   HOGENOM; HOG000168003; -.
DR   KO; K01711; -.
DR   OMA; TDCLYLG; -.
DR   OrthoDB; 43299at2157; -.
DR   BioCyc; MMAH547558:G1GHT-81-MONOMER; -.
DR   Proteomes; UP000001059; Chromosome.
DR   GO; GO:0008446; F:GDP-mannose 4,6-dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0070401; F:NADP+ binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019673; P:GDP-mannose metabolic process; IEA:InterPro.
DR   HAMAP; MF_00955; GDP_Man_dehydratase; 1.
DR   InterPro; IPR006368; GDP_Man_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01472; gmd; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5E8V9.
DR   SWISS-2DPAGE; D5E8V9.
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_00955, ECO:0000313|EMBL:ADE35618.1};
KW   NADP {ECO:0000256|HAMAP-Rule:MF_00955};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001059}.
FT   DOMAIN          6..336
FT                   /note="NAD(P)-bd_dom"
FT                   /evidence="ECO:0000259|Pfam:PF16363"
SQ   SEQUENCE   345 AA;  39143 MW;  728611E250C8BB5B CRC64;
     MPKVALVTGI TGQDGAYLAE FLLDKGYIVH GIKRRSSSFN TARIDHLYKD PHERNVNFFM
     HYGDLTDSTN LIRIIQETQP DEIYNLAAQS HVQVSFETPE YTANSDAIGS LRLLEAIRIL
     GMEKKVKFYQ ASTSELYGKV QEIPQSETTP FYPRSPYAAA KLYAYWITIN YREAYGMYAC
     NGILFNHESP IRGETFVTRK ITMAVANIKK GLQERLYLGN LDAKRDWGFA GDYVKAMWLM
     LQQDEPEDFV IATGETHSVR EFVELAFREV GIDIEWKGEG VDEVGVDSET GNVLVEIDPA
     YYRPTEVELL LGNPSKAREK LGWNSNVGFE ELVKMMVKGD MGKLL
//

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