(data stored in SCRATCH zone)

SWISSPROT: D5EA39_METMS

ID   D5EA39_METMS            Unreviewed;       264 AA.
AC   D5EA39;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   11-DEC-2019, entry version 53.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase E {ECO:0000256|HAMAP-Rule:MF_01547};
DE            EC=2.1.1.166 {ECO:0000256|HAMAP-Rule:MF_01547};
DE   AltName: Full=23S rRNA Um2552 methyltransferase {ECO:0000256|HAMAP-Rule:MF_01547};
DE   AltName: Full=rRNA (uridine-2'-O-)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01547};
GN   Name=rlmE {ECO:0000256|HAMAP-Rule:MF_01547};
GN   OrderedLocusNames=Mmah_0513 {ECO:0000313|EMBL:ADE36040.1};
OS   Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanohalophilus.
OX   NCBI_TaxID=547558 {ECO:0000313|EMBL:ADE36040.1, ECO:0000313|Proteomes:UP000001059};
RN   [1] {ECO:0000313|EMBL:ADE36040.1, ECO:0000313|Proteomes:UP000001059}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35705 / DSM 5219 / SLP
RC   {ECO:0000313|Proteomes:UP000001059};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA   Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M.,
RA   Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D.,
RA   Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.;
RT   "The complete genome of Methanohalophilus mahii DSM 5219.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically methylates the uridine in position 2552 of 23S
CC       rRNA at the 2'-O position of the ribose in the fully assembled 50S
CC       ribosomal subunit. {ECO:0000256|HAMAP-Rule:MF_01547}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-
CC         methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42720, Rhea:RHEA-COMP:10202, Rhea:RHEA-COMP:10203,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.166;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01547};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01547}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase RlmE family. {ECO:0000256|HAMAP-
CC       Rule:MF_01547}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001994; ADE36040.1; -; Genomic_DNA.
DR   RefSeq; WP_013036983.1; NC_014002.1.
DR   EnsemblBacteria; ADE36040; ADE36040; Mmah_0513.
DR   GeneID; 8982654; -.
DR   KEGG; mmh:Mmah_0513; -.
DR   eggNOG; arCOG00079; Archaea.
DR   eggNOG; arCOG01641; Archaea.
DR   eggNOG; COG0293; LUCA.
DR   eggNOG; COG3269; LUCA.
DR   HOGENOM; HOG000162366; -.
DR   KO; K02427; -.
DR   OMA; HRQTDHL; -.
DR   OrthoDB; 105478at2157; -.
DR   BioCyc; MMAH547558:G1GHT-515-MONOMER; -.
DR   Proteomes; UP000001059; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008650; F:rRNA (uridine-2'-O-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01547; RNA_methyltr_E; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR015507; rRNA-MeTfrase_E.
DR   InterPro; IPR002877; rRNA_MeTrfase_FtsJ_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR002792; TRAM_dom.
DR   Pfam; PF01728; FtsJ; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   PIRSF; PIRSF005461; 23S_rRNA_mtase; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS50926; TRAM; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5EA39.
DR   SWISS-2DPAGE; D5EA39.
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01547};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01547,
KW   ECO:0000313|EMBL:ADE36040.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001059};
KW   rRNA processing {ECO:0000256|HAMAP-Rule:MF_01547};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_01547,
KW   ECO:0000256|PIRSR:PIRSR005461-1};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01547, ECO:0000313|EMBL:ADE36040.1}.
FT   DOMAIN          196..254
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        149
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547,
FT                   ECO:0000256|PIRSR:PIRSR005461-1"
FT   BINDING         50
FT                   /note="S-adenosyl-L-methionine; via amide nitrogen"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547"
FT   BINDING         52
FT                   /note="S-adenosyl-L-methionine; via amide nitrogen"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547"
FT   BINDING         68
FT                   /note="S-adenosyl-L-methionine"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547"
FT   BINDING         84
FT                   /note="S-adenosyl-L-methionine"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547"
FT   BINDING         109
FT                   /note="S-adenosyl-L-methionine"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01547"
SQ   SEQUENCE   264 AA;  29735 MW;  0C4F251AB297896E CRC64;
     MARHRRDTYY WRAKAEGYRS RAAYKLQQIN KKFNVIREDS DVVDLGAAPG GWLEVARELT
     KRKVVGVDIL RIKPMDRITI IRGDITREET VSQIKEAVGE DGADAVICDA APNLSGNWNL
     DHARSIALAE SALECATRIL KPQGNFVVKV FQGDMFKEYL DKVRGEFTYV RAHSPEASRS
     ESAEIYVVGK KFLTAPIRKG EEYDVVIERM GSGGDGTAFV EGFVVFICDT EKGEKVRIKV
     RDVKPNFAFA DVIQRLESPE EEEK
//

If you have problems or comments...

PBIL Back to PBIL home page