(data stored in SCRATCH zone)

SWISSPROT: D5UQK4_TSUPD

ID   D5UQK4_TSUPD            Unreviewed;       558 AA.
AC   D5UQK4;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   08-MAY-2019, entry version 59.
DE   RecName: Full=Phosphoenolpyruvate-protein phosphotransferase {ECO:0000256|PIRNR:PIRNR000732};
DE            EC=2.7.3.9 {ECO:0000256|PIRNR:PIRNR000732};
DE   AltName: Full=Phosphotransferase system, enzyme I {ECO:0000256|PIRNR:PIRNR000732};
GN   OrderedLocusNames=Tpau_0183 {ECO:0000313|EMBL:ADG76837.1};
OS   Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / JCM 10117 /
OS   NBRC 16120 / NCTC 13040) (Corynebacterium paurometabolum).
OC   Bacteria; Actinobacteria; Corynebacteriales; Tsukamurellaceae;
OC   Tsukamurella.
OX   NCBI_TaxID=521096 {ECO:0000313|EMBL:ADG76837.1, ECO:0000313|Proteomes:UP000001213};
RN   [1] {ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K.,
RA   Ivanova N., Mikhailova N., Munk A.C., Brettin T., Detter J.C.,
RA   Tapia R., Han C., Larimer F., Land M., Hauser L., Markowitz V.,
RA   Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Jando M., Brambilla E.,
RA   Klenk H.-P., Eisen J.A.;
RT   "The complete chromosome of Tsukamurella paurometabola DSM 20162.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADG76837.1, ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RX   PubMed=21886861;
RA   Munk A.C., Lapidus A., Lucas S., Nolan M., Tice H., Cheng J.F.,
RA   Del Rio T.G., Goodwin L., Pitluck S., Liolios K., Huntemann M.,
RA   Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Tapia R., Han C., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Yasawong M., Brambilla E.M., Rohde M.,
RA   Sikorski J., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A.,
RA   Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Tsukamurella paurometabola type strain
RT   (no. 33).";
RL   Stand. Genomic Sci. 4:342-351(2011).
CC   -!- FUNCTION: General (non sugar-specific) component of the
CC       phosphoenolpyruvate-dependent sugar phosphotransferase system
CC       (sugar PTS). This major carbohydrate active-transport system
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane.
CC       Enzyme I transfers the phosphoryl group from phosphoenolpyruvate
CC       (PEP) to the phosphoryl carrier protein (HPr).
CC       {ECO:0000256|PIRNR:PIRNR000732}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-
CC         phospho-L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880,
CC         Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:29979, ChEBI:CHEBI:58702, ChEBI:CHEBI:64837;
CC         EC=2.7.3.9; Evidence={ECO:0000256|PIRNR:PIRNR000732};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000732,
CC         ECO:0000256|PIRSR:PIRSR000732-3};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR000732}.
CC   -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC       {ECO:0000256|PIRNR:PIRNR000732, ECO:0000256|SAAS:SAAS00563041}.
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DR   EMBL; CP001966; ADG76837.1; -; Genomic_DNA.
DR   RefSeq; WP_013124892.1; NC_014158.1.
DR   STRING; 521096.Tpau_0183; -.
DR   EnsemblBacteria; ADG76837; ADG76837; Tpau_0183.
DR   KEGG; tpr:Tpau_0183; -.
DR   eggNOG; ENOG4105BZ3; Bacteria.
DR   eggNOG; COG1080; LUCA.
DR   HOGENOM; HOG000278513; -.
DR   KO; K08483; -.
DR   OMA; CNAEWAL; -.
DR   OrthoDB; 467393at2; -.
DR   BioCyc; TPAU521096:G1GKO-182-MONOMER; -.
DR   Proteomes; UP000001213; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.274.10; -; 1.
DR   Gene3D; 3.20.20.60; -; 1.
DR   InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR   InterPro; IPR018274; PEP_util_AS.
DR   InterPro; IPR000121; PEP_util_C.
DR   InterPro; IPR023151; PEP_util_CS.
DR   InterPro; IPR036637; Phosphohistidine_dom_sf.
DR   InterPro; IPR024692; PTS_EI.
DR   InterPro; IPR006318; PTS_EI-like.
DR   InterPro; IPR008731; PTS_EIN.
DR   InterPro; IPR036618; PtsI_HPr-bd_sf.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   Pfam; PF05524; PEP-utilisers_N; 1.
DR   Pfam; PF00391; PEP-utilizers; 1.
DR   Pfam; PF02896; PEP-utilizers_C; 1.
DR   PIRSF; PIRSF000732; PTS_enzyme_I; 1.
DR   SUPFAM; SSF47831; SSF47831; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   SUPFAM; SSF52009; SSF52009; 1.
DR   TIGRFAMs; TIGR01417; PTS_I_fam; 1.
DR   PROSITE; PS00742; PEP_ENZYMES_2; 1.
DR   PROSITE; PS00370; PEP_ENZYMES_PHOS_SITE; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5UQK4.
DR   SWISS-2DPAGE; D5UQK4.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001213};
KW   Cytoplasm {ECO:0000256|PIRNR:PIRNR000732};
KW   Kinase {ECO:0000256|PIRNR:PIRNR000732};
KW   Magnesium {ECO:0000256|PIRNR:PIRNR000732,
KW   ECO:0000256|PIRSR:PIRSR000732-3};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000732,
KW   ECO:0000256|PIRSR:PIRSR000732-3};
KW   Phosphotransferase system {ECO:0000256|PIRNR:PIRNR000732};
KW   Pyruvate {ECO:0000313|EMBL:ADG76837.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001213};
KW   Sugar transport {ECO:0000256|PIRNR:PIRNR000732};
KW   Transferase {ECO:0000256|PIRNR:PIRNR000732,
KW   ECO:0000256|SAAS:SAAS00451307, ECO:0000313|EMBL:ADG76837.1};
KW   Transport {ECO:0000256|PIRNR:PIRNR000732}.
FT   DOMAIN       13    130       PEP-utilisers_N. {ECO:0000259|Pfam:
FT                                PF05524}.
FT   DOMAIN      158    228       PEP-utilizers. {ECO:0000259|Pfam:
FT                                PF00391}.
FT   DOMAIN      261    520       PEP-utilizers_C. {ECO:0000259|Pfam:
FT                                PF02896}.
FT   REGION      442    443       PEP binding. {ECO:0000256|PIRSR:
FT                                PIRSR000732-2}.
FT   ACT_SITE    193    193       Tele-phosphohistidine intermediate.
FT                                {ECO:0000256|PIRSR:PIRSR000732-1}.
FT   ACT_SITE    490    490       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR000732-1}.
FT   METAL       419    419       Magnesium. {ECO:0000256|PIRSR:
FT                                PIRSR000732-3}.
FT   METAL       443    443       Magnesium. {ECO:0000256|PIRSR:
FT                                PIRSR000732-3}.
FT   BINDING     295    295       PEP. {ECO:0000256|PIRSR:PIRSR000732-2}.
FT   BINDING     331    331       PEP. {ECO:0000256|PIRSR:PIRSR000732-2}.
FT   BINDING     453    453       PEP. {ECO:0000256|PIRSR:PIRSR000732-2}.
SQ   SEQUENCE   558 AA;  56681 MW;  CB6FC67E8DBDCA5E CRC64;
     MSSQQSTVTV LAGTAVVPGV AYAPVMWPGE RPELPAVGGE LPETERDAEA VRLSSAARTV
     AGRLRERASQ ATGAASEVLA ATAGLASDKA WLSAAEKLIR SGSPAEGAVA AATEQFADMF
     TTIGGLMAER VTDLRDVRDR VLAELLGRPE PGVPTPTAPS VLLADDLAPA DTAGLDPALV
     VGIGTRLGGP TSHTAIIARQ LGIPCIVGLA EAGTVEAGTL VLLDGAEGTV TVAPPADGAR
     ARVAEAMRMA EDVARWRGPG RTSDGTAVAI LANVQDGPSA RTAAERQVEG VGLFRTELAF
     LNRADEPSVD EQQALYREVI DAFPAGKVVI RTLDAGSDKP LKFIDHGHED NPALGIRGIR
     IAATDPGLLE RQLDAIAAAA TGAAEAPWVM APMIATAAEA RSFAAQCRAR GLTPGVMIEV
     PAAALCAEAV LAEVDFVSVG TNDLTQYAMA ADRMSSELAA LTDPWQPAVL RLIRLTADAG
     AGAGKPVGVC GEAAADPLLA CVLVGLGVTS LSCAAGAAAA VGARLGGVTL QQCRWAAEAA
     VASADPQAAR DAARRALS
//

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