(data stored in SCRATCH zone)

SWISSPROT: D5URF6_TSUPD

ID   D5URF6_TSUPD            Unreviewed;       267 AA.
AC   D5URF6;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   08-MAY-2019, entry version 52.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01057};
DE            EC=2.1.1.33 {ECO:0000256|HAMAP-Rule:MF_01057};
DE   AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01057};
DE   AltName: Full=tRNA(m7G46)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01057};
GN   Name=trmB {ECO:0000256|HAMAP-Rule:MF_01057};
GN   OrderedLocusNames=Tpau_0366 {ECO:0000313|EMBL:ADG77009.1};
OS   Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / JCM 10117 /
OS   NBRC 16120 / NCTC 13040) (Corynebacterium paurometabolum).
OC   Bacteria; Actinobacteria; Corynebacteriales; Tsukamurellaceae;
OC   Tsukamurella.
OX   NCBI_TaxID=521096 {ECO:0000313|EMBL:ADG77009.1, ECO:0000313|Proteomes:UP000001213};
RN   [1] {ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K.,
RA   Ivanova N., Mikhailova N., Munk A.C., Brettin T., Detter J.C.,
RA   Tapia R., Han C., Larimer F., Land M., Hauser L., Markowitz V.,
RA   Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Jando M., Brambilla E.,
RA   Klenk H.-P., Eisen J.A.;
RT   "The complete chromosome of Tsukamurella paurometabola DSM 20162.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADG77009.1, ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RX   PubMed=21886861;
RA   Munk A.C., Lapidus A., Lucas S., Nolan M., Tice H., Cheng J.F.,
RA   Del Rio T.G., Goodwin L., Pitluck S., Liolios K., Huntemann M.,
RA   Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Tapia R., Han C., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Yasawong M., Brambilla E.M., Rohde M.,
RA   Sikorski J., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A.,
RA   Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Tsukamurella paurometabola type strain
RT   (no. 33).";
RL   Stand. Genomic Sci. 4:342-351(2011).
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_01057}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-
CC         methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-
CC         COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=2.1.1.33;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01057};
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_01057}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC       methyltransferase superfamily. TrmB family. {ECO:0000256|HAMAP-
CC       Rule:MF_01057}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01057}.
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DR   EMBL; CP001966; ADG77009.1; -; Genomic_DNA.
DR   STRING; 521096.Tpau_0366; -.
DR   EnsemblBacteria; ADG77009; ADG77009; Tpau_0366.
DR   KEGG; tpr:Tpau_0366; -.
DR   eggNOG; ENOG4105CZ1; Bacteria.
DR   eggNOG; COG0220; LUCA.
DR   HOGENOM; HOG000073968; -.
DR   KO; K03439; -.
DR   OMA; PDPWHKS; -.
DR   BioCyc; TPAU521096:G1GKO-349-MONOMER; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001213; Chromosome.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01057; tRNA_methyltr_TrmB; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb.
DR   PANTHER; PTHR23417; PTHR23417; 1.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00091; TIGR00091; 1.
DR   PROSITE; PS51625; SAM_MT_TRMB; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5URF6.
DR   SWISS-2DPAGE; D5URF6.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001213};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01057,
KW   ECO:0000256|SAAS:SAAS00466135, ECO:0000313|EMBL:ADG77009.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001213};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_01057,
KW   ECO:0000256|SAAS:SAAS00466123};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01057,
KW   ECO:0000256|SAAS:SAAS00097766, ECO:0000313|EMBL:ADG77009.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_01057}.
FT   BINDING      87     87       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_01057}.
FT   BINDING     112    112       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_01057}.
FT   BINDING     139    139       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_01057}.
FT   BINDING     162    162       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_01057}.
FT   BINDING     166    166       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01057}.
FT   BINDING     198    198       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01057}.
SQ   SEQUENCE   267 AA;  29457 MW;  06961E6051C5EE66 CRC64;
     MESVPESQDP RAEHRRLYPR VTSFRSRRGA LTPSQQRAMD EMWPTIGRDI PPARPVDGAP
     NEDAFPAPSH PLDAPAWFGR TAPLIVEIGC GTGTSTVAMA EAEPERDVLA VEVYLPGIAQ
     LVGGVQRQGL TNVRVVRGDG VAVLEDMIAP GSLEGVRVYF PDPWPKARHH KRRLLQPRMF
     SLIADRLRVG GILHVATDHA DYAEAIAEVA PSEPLLRPVE LPPRLADANL PISVDRPVTK
     FQDKAAQVGR EIHEFVWERV VEEGAHR
//

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