(data stored in SCRATCH zone)

SWISSPROT: D5USE4_TSUPD

ID   D5USE4_TSUPD            Unreviewed;       598 AA.
AC   D5USE4;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   08-MAY-2019, entry version 57.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000256|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000256|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000256|HAMAP-Rule:MF_00177};
GN   OrderedLocusNames=Tpau_0571 {ECO:0000313|EMBL:ADG77211.1};
OS   Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / JCM 10117 /
OS   NBRC 16120 / NCTC 13040) (Corynebacterium paurometabolum).
OC   Bacteria; Actinobacteria; Corynebacteriales; Tsukamurellaceae;
OC   Tsukamurella.
OX   NCBI_TaxID=521096 {ECO:0000313|EMBL:ADG77211.1, ECO:0000313|Proteomes:UP000001213};
RN   [1] {ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K.,
RA   Ivanova N., Mikhailova N., Munk A.C., Brettin T., Detter J.C.,
RA   Tapia R., Han C., Larimer F., Land M., Hauser L., Markowitz V.,
RA   Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Jando M., Brambilla E.,
RA   Klenk H.-P., Eisen J.A.;
RT   "The complete chromosome of Tsukamurella paurometabola DSM 20162.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADG77211.1, ECO:0000313|Proteomes:UP000001213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8368 / DSM 20162 / JCM 10117 / NBRC 16120 / NCTC 13040
RC   {ECO:0000313|Proteomes:UP000001213};
RX   PubMed=21886861;
RA   Munk A.C., Lapidus A., Lucas S., Nolan M., Tice H., Cheng J.F.,
RA   Del Rio T.G., Goodwin L., Pitluck S., Liolios K., Huntemann M.,
RA   Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Tapia R., Han C., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Yasawong M., Brambilla E.M., Rohde M.,
RA   Sikorski J., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A.,
RA   Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Tsukamurella paurometabola type strain
RT   (no. 33).";
RL   Stand. Genomic Sci. 4:342-351(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78529,
CC         ChEBI:CHEBI:456215; EC=6.1.1.6; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00177}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00177}.
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DR   EMBL; CP001966; ADG77211.1; -; Genomic_DNA.
DR   STRING; 521096.Tpau_0571; -.
DR   EnsemblBacteria; ADG77211; ADG77211; Tpau_0571.
DR   KEGG; tpr:Tpau_0571; -.
DR   eggNOG; ENOG4105DK7; Bacteria.
DR   eggNOG; COG1384; LUCA.
DR   HOGENOM; HOG000226938; -.
DR   KO; K04566; -.
DR   OMA; DWPMRWA; -.
DR   BioCyc; TPAU521096:G1GKO-554-MONOMER; -.
DR   Proteomes; UP000001213; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 1.10.10.860; -; 1.
DR   Gene3D; 2.30.30.300; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR008925; aa-tRNA-synth_I_codon-bd.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR023386; Lys-tRNA-ligase_insertion_sf.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5USE4.
DR   SWISS-2DPAGE; D5USE4.
KW   Aminoacyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00177,
KW   ECO:0000313|EMBL:ADG77211.1};
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001213};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00177, ECO:0000313|EMBL:ADG77211.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001213}.
FT   MOTIF        40     48       "HIGH" region. {ECO:0000256|HAMAP-Rule:
FT                                MF_00177}.
FT   MOTIF       321    325       "KMSKS" region. {ECO:0000256|HAMAP-Rule:
FT                                MF_00177}.
SQ   SEQUENCE   598 AA;  65542 MW;  A231C1B0D639095D CRC64;
     MTVAPVNAPL DDWVTRLADE AVAFAEQNDA PVKVASGISP SGPIHLGNLR EVMVPHLVAD
     ELRRRGRTVE HIISWDDFDR FRKVPTIDGV DETWEQHIGK PLTRVPAPYG SDAANWAEHF
     RRELETALVE LGVTYRGISQ TQMYSSGAYI DQVLHAMAER QRIDAILERY RTLDKAKKGG
     VTVDQPEADA EAADAASGSA DEADGSGDSG YYPYKPYCSV CGTDFTTVIG YDDESTALKY
     QCRCGHTETV ILREHTDGKL VWKVDWPMRW AFEKITFEPS GVDHQSPGSS FAVGKDVAPI
     FGWRRPLGPM YAFVGIRGMA KMSSSKGGVP TAAIALRYLE PPLLRWLYGR KKPNQSFDVA
     LDGELPRTYD EWDALTRKVA GDKAQPGDIA AYARAASVAD PVEGQVRALA LTPRTMPYRT
     LASVVDITTG DDEQTLRILG ALEPDEPLTG LDVLRPRLDK ATTWVAEQMP AQERTVVRTA
     PDTDLLRGLT DDERAALRLL LDGSGTPAPE GLGRLEDDWT LAGITHQVYG VAKVQRGLGA
     DQIVKGDKEL GAAQRAFFVL LYRLLVGGDT GPRLPTLLLA IGAKRVRELV ASDRIQPN
//

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