(data stored in SCRATCH zone)

SWISSPROT: D7A1W7_STAND

ID   D7A1W7_STAND            Unreviewed;       283 AA.
AC   D7A1W7;
DT   10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT   10-AUG-2010, sequence version 1.
DT   08-MAY-2019, entry version 59.
DE   RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] {ECO:0000256|PIRNR:PIRNR000094};
DE            EC=1.3.1.9 {ECO:0000256|PIRNR:PIRNR000094};
GN   OrderedLocusNames=Snov_0248 {ECO:0000313|EMBL:ADH87583.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 /
OS   NBRC 12443 / NCIB 9113).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283 {ECO:0000313|EMBL:ADH87583.1, ECO:0000313|Proteomes:UP000006633};
RN   [1] {ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Beatson S., Kappler U.,
RA   Woyke T.;
RT   "Complete sequence of Starkeya novella DSM 506.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADH87583.1, ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RX   PubMed=23450099; DOI=10.4056/sigs.3006378;
RA   Kappler U., Davenport K., Beatson S., Lucas S., Lapidus A.,
RA   Copeland A., Berry K.W., Glavina Del Rio T., Hammon N., Dalin E.,
RA   Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L.A., Han C.,
RA   Tapia R., Detter J.C., Chang Y.J., Jeffries C.D., Land M., Hauser L.,
RA   Kyrpides N.C., Goker M., Ivanova N., Klenk H.P., Woyke T.;
RT   "Complete genome sequence of the facultatively chemolithoautotrophic
RT   and methylotrophic alpha Proteobacterium Starkeya novella type strain
RT   (ATCC 8093(T)).";
RL   Stand. Genomic Sci. 7:44-58(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP]
CC         + H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925,
CC         Rhea:RHEA-COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:78784, ChEBI:CHEBI:78785;
CC         EC=1.3.1.9; Evidence={ECO:0000256|PIRNR:PIRNR000094};
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. FabI subfamily. {ECO:0000256|PIRNR:PIRNR000094}.
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DR   EMBL; CP002026; ADH87583.1; -; Genomic_DNA.
DR   STRING; 639283.Snov_0248; -.
DR   EnsemblBacteria; ADH87583; ADH87583; Snov_0248.
DR   KEGG; sno:Snov_0248; -.
DR   eggNOG; ENOG4105CSJ; Bacteria.
DR   eggNOG; COG0623; LUCA.
DR   KO; K00208; -.
DR   OMA; NHSIAWG; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0016631; F:enoyl-[acyl-carrier-protein] reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd05372; ENR_SDR; 1.
DR   InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PANTHER; PTHR43159:SF2; PTHR43159:SF2; 1.
DR   PIRSF; PIRSF000094; Enoyl-ACP_rdct; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
DR   PRODOM; D7A1W7.
DR   SWISS-2DPAGE; D7A1W7.
KW   Complete proteome {ECO:0000313|Proteomes:UP000006633};
KW   Fatty acid biosynthesis {ECO:0000256|PIRNR:PIRNR000094};
KW   Fatty acid metabolism {ECO:0000256|PIRNR:PIRNR000094};
KW   Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR000094};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR000094};
KW   NAD {ECO:0000256|PIRNR:PIRNR000094, ECO:0000256|PIRSR:PIRSR000094-3};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000094};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006633}.
FT   NP_BIND      24     25       NAD. {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   NP_BIND      69     70       NAD. {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   NP_BIND     196    200       NAD. {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   ACT_SITE    150    150       Proton acceptor. {ECO:0000256|PIRSR:
FT                                PIRSR000094-1}.
FT   ACT_SITE    160    160       Proton acceptor. {ECO:0000256|PIRSR:
FT                                PIRSR000094-1}.
FT   BINDING      18     18       NAD; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   BINDING      45     45       NAD. {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   BINDING      97     97       NAD; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR000094-3}.
FT   BINDING     167    167       NAD. {ECO:0000256|PIRSR:PIRSR000094-3}.
SQ   SEQUENCE   283 AA;  30087 MW;  105E66C5731B2EB5 CRC64;
     MGLMQGNLMN GKRGLIMGVA NDHSIAWGIA KTLAAQGAEL AFTYQGEALG RRVRPLAESL
     GSDTLISCDV EDLDSVDAVF AALKEKWGSI DFLVHAIGFS DKNELKGRYA DTTRANFTRT
     MVISCFSFTE LAKRAAAIMP NGGSVITLTY GGSTRVMPNY NVMGVAKAAL EASVRYLAAD
     YGPQGIRVNA ISAGPIRTLA GAGIADARLM FNYQKRNAPL RRTVTIDEVG GSALYLLSDL
     SSGVTGEVHF VDSGYNIISM PHPDALKTIE DAEERAAGEV AAE
//

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