(data stored in SCRATCH zone)

SWISSPROT: D7A482_STAND

ID   D7A482_STAND            Unreviewed;       552 AA.
AC   D7A482;
DT   10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT   10-AUG-2010, sequence version 1.
DT   08-MAY-2019, entry version 61.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000256|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000256|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000256|HAMAP-Rule:MF_00177};
GN   OrderedLocusNames=Snov_0569 {ECO:0000313|EMBL:ADH87902.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 /
OS   NBRC 12443 / NCIB 9113).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283 {ECO:0000313|EMBL:ADH87902.1, ECO:0000313|Proteomes:UP000006633};
RN   [1] {ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Beatson S., Kappler U.,
RA   Woyke T.;
RT   "Complete sequence of Starkeya novella DSM 506.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADH87902.1, ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RX   PubMed=23450099; DOI=10.4056/sigs.3006378;
RA   Kappler U., Davenport K., Beatson S., Lucas S., Lapidus A.,
RA   Copeland A., Berry K.W., Glavina Del Rio T., Hammon N., Dalin E.,
RA   Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L.A., Han C.,
RA   Tapia R., Detter J.C., Chang Y.J., Jeffries C.D., Land M., Hauser L.,
RA   Kyrpides N.C., Goker M., Ivanova N., Klenk H.P., Woyke T.;
RT   "Complete genome sequence of the facultatively chemolithoautotrophic
RT   and methylotrophic alpha Proteobacterium Starkeya novella type strain
RT   (ATCC 8093(T)).";
RL   Stand. Genomic Sci. 7:44-58(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78529,
CC         ChEBI:CHEBI:456215; EC=6.1.1.6; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00177}.
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DR   EMBL; CP002026; ADH87902.1; -; Genomic_DNA.
DR   RefSeq; WP_013165407.1; NC_014217.1.
DR   STRING; 639283.Snov_0569; -.
DR   EnsemblBacteria; ADH87902; ADH87902; Snov_0569.
DR   KEGG; sno:Snov_0569; -.
DR   eggNOG; ENOG4105DK7; Bacteria.
DR   eggNOG; COG1384; LUCA.
DR   HOGENOM; HOG000140447; -.
DR   KO; K04566; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 256927at2; -.
DR   BioCyc; SNOV639283:G1GLM-574-MONOMER; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 2.30.30.300; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR008925; aa-tRNA-synth_I_codon-bd.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR023386; Lys-tRNA-ligase_insertion_sf.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
DR   PRODOM; D7A482.
DR   SWISS-2DPAGE; D7A482.
KW   Aminoacyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00177,
KW   ECO:0000313|EMBL:ADH87902.1};
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Complete proteome {ECO:0000313|Proteomes:UP000006633};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00177, ECO:0000313|EMBL:ADH87902.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00177};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006633}.
FT   MOTIF        52     60       "HIGH" region. {ECO:0000256|HAMAP-Rule:
FT                                MF_00177}.
FT   MOTIF       301    305       "KMSKS" region. {ECO:0000256|HAMAP-Rule:
FT                                MF_00177}.
FT   BINDING     304    304       ATP. {ECO:0000256|HAMAP-Rule:MF_00177}.
SQ   SEQUENCE   552 AA;  61171 MW;  96FD6B9119D5FBF0 CRC64;
     MTATAQVEDV AAELHELAEN AGAWPFEEAR KIVERLKRKP KSEVLFETGY GPSGLPHIGT
     FGEVARTTMV RHAFRVLTED KIPTRLLCFS DDMDGMRKIP ENVPDPVALR PYLQMPLTAV
     PNPFGGGFAS FGDHNNAMLR RFLDTFGFDY EFASATDYYK SGKLDAVLLK AAEKYDAIME
     VMLPTLGEER QATYSPFLPI SPTSGRVLYV PLKEVNAKAG TITFTDEDGV DKTVPVTGGQ
     VKLQWKPDFG ARWAALDVDF EMFGKDHQTN APIYDAICEI LGGTAPEHYV YELFLDENGQ
     KISKSKGNGL TIDEWLTYAS PESLALYMFQ SPRSAKKLHF DVIPKAVDEY FNFLARYPVQ
     DWKNRLGNPV WHIHSGNPPV EELPLTFGLM LKLASASNAE DKSVLWRFIQ RYAPGTSPAT
     HPTVDKLADY AVRYFVDRVK PRRTFRLPDE VEAKALAALD AALAPLDGSD RDAIQNAVLD
     VGRAEPRYQD MKRKGPEGGP GVSFLWFSAL YELLLGEKEG PRFGSFVAAY GIPETRALIA
     RALAGELAAG AA
//

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