(data stored in ACNUC8465 zone)

SWISSPROT: D8GPN4_CLOLD

ID   D8GPN4_CLOLD            Unreviewed;       450 AA.
AC   D8GPN4;
DT   05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2010, sequence version 1.
DT   08-MAY-2019, entry version 56.
DE   SubName: Full=tRNA (Uracil-5-)-methyltransferase, SAM-dependent {ECO:0000313|EMBL:ADK13943.1};
DE            EC=2.1.1.35 {ECO:0000313|EMBL:ADK13943.1};
GN   OrderedLocusNames=CLJU_c08750 {ECO:0000313|EMBL:ADK13943.1};
OS   Clostridium ljungdahlii (strain ATCC 55383 / DSM 13528 / PETC).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=748727 {ECO:0000313|EMBL:ADK13943.1, ECO:0000313|Proteomes:UP000001656};
RN   [1] {ECO:0000313|EMBL:ADK13943.1, ECO:0000313|Proteomes:UP000001656}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 55383 / DSM 13528 /
RC   PETC {ECO:0000313|Proteomes:UP000001656};
RX   PubMed=20616070; DOI=10.1073/pnas.1004716107;
RA   Kopke M., Held C., Hujer S., Liesegang H., Wiezer A., Wollherr A.,
RA   Ehrenreich A., Liebl W., Gottschalk G., Durre P.;
RT   "Clostridium ljungdahlii represents a microbial production platform
RT   based on syngas.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:13087-13092(2010).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC       methyltransferase superfamily. RNA M5U methyltransferase family.
CC       {ECO:0000256|PROSITE-ProRule:PRU01024,
CC       ECO:0000256|SAAS:SAAS00561307}.
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DR   EMBL; CP001666; ADK13943.1; -; Genomic_DNA.
DR   RefSeq; WP_013237540.1; NZ_LITS01000009.1.
DR   STRING; 748727.CLJU_c08750; -.
DR   EnsemblBacteria; ADK13943; ADK13943; CLJU_c08750.
DR   KEGG; clj:CLJU_c08750; -.
DR   PATRIC; fig|748727.19.peg.3625; -.
DR   eggNOG; ENOG4105BZT; Bacteria.
DR   eggNOG; COG2265; LUCA.
DR   HOGENOM; HOG000029870; -.
DR   OMA; SCQWLEK; -.
DR   OrthoDB; 1421660at2; -.
DR   BioCyc; CLJU748727:G1GM0-861-MONOMER; -.
DR   Proteomes; UP000001656; Chromosome.
DR   GO; GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; D8GPN4.
DR   SWISS-2DPAGE; D8GPN4.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001656};
KW   Methyltransferase {ECO:0000256|PROSITE-ProRule:PRU01024,
KW   ECO:0000256|SAAS:SAAS00234999, ECO:0000313|EMBL:ADK13943.1};
KW   S-adenosyl-L-methionine {ECO:0000256|PROSITE-ProRule:PRU01024,
KW   ECO:0000256|SAAS:SAAS00235009};
KW   Transferase {ECO:0000256|PROSITE-ProRule:PRU01024,
KW   ECO:0000256|SAAS:SAAS00506843, ECO:0000313|EMBL:ADK13943.1}.
FT   ACT_SITE    408    408       {ECO:0000256|PROSITE-ProRule:PRU10015}.
FT   ACT_SITE    408    408       Nucleophile. {ECO:0000256|PROSITE-
FT                                ProRule:PRU01024}.
FT   BINDING     286    286       S-adenosyl-L-methionine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU01024}.
FT   BINDING     315    315       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen. {ECO:0000256|PROSITE-ProRule:
FT                                PRU01024}.
FT   BINDING     336    336       S-adenosyl-L-methionine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU01024}.
FT   BINDING     381    381       S-adenosyl-L-methionine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU01024}.
SQ   SEQUENCE   450 AA;  50799 MW;  2C170A04FEC3F962 CRC64;
     MKKGSEYEFF IEELQFPAIG IAKKDDMDVL IKNTVPGQKV KARITKKTKH YLEAKVTEIL
     ENVNYAVDPF CPHFNLCGGC TSQFIPYDKQ LELKEKQVLD LFEKASICDF EFLGIEKSPE
     KTGYRNKMEF SFGDEQRGGQ LTLGMHIKGR SFGVVNVDNC NLVDADFTLI LDKVVTYFRE
     KNLPYYKIKN HEGYLRNLVL RKGRNTGEIL INIVTTSQIQ FDLDGFNKLL QVLNYKGTLV
     GILHTVNDNL SDMVKADNVE ILYGRDYIME EILGLKFKIS PESFFQTNSR GTEKLYAMIK
     DFLGDASSKT VFDLYCGTGT IGQVLSSSVN KVIGVELIEK AVASANENAK LNNIKNCRFI
     AGDVKDVLNT LEDKPDIIIL DPPRPGVHPK ALKYVIDFNA PEIIYVSCNP KTLVQDLNVL
     IDAGYNVDKV KLMDMFPHTP HVETVVKLSK
//

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