(data stored in ACNUC10043 zone)

SWISSPROT: E1RK60_METP4

ID   E1RK60_METP4            Unreviewed;       422 AA.
AC   E1RK60;
DT   30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT   30-NOV-2010, sequence version 1.
DT   11-DEC-2019, entry version 48.
DE   RecName: Full=UDP-glucose 6-dehydrogenase {ECO:0000256|PIRNR:PIRNR000124};
DE            EC=1.1.1.22 {ECO:0000256|PIRNR:PIRNR000124};
GN   OrderedLocusNames=Mpet_1016 {ECO:0000313|EMBL:ADN35783.1};
OS   Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847)
OS   (Methanoplanus petrolearius).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanomicrobiaceae; Methanolacinia.
OX   NCBI_TaxID=679926 {ECO:0000313|EMBL:ADN35783.1, ECO:0000313|Proteomes:UP000006565};
RN   [1] {ECO:0000313|EMBL:ADN35783.1, ECO:0000313|Proteomes:UP000006565}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 11571 / OCM 486 / SEBR 4847
RC   {ECO:0000313|Proteomes:UP000006565};
RX   PubMed=21304750;
RA   Brambilla E., Djao O.D., Daligault H., Lapidus A., Lucas S., Hammon N.,
RA   Nolan M., Tice H., Cheng J.F., Han C., Tapia R., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Rohde M.,
RA   Spring S., Sikorski J., Goker M., Woyke T., Bristow J., Eisen J.A.,
RA   Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Methanoplanus petrolearius type strain (SEBR
RT   4847).";
RL   Stand. Genomic Sci. 3:203-211(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-
CC         alpha-D-glucuronate; Xref=Rhea:RHEA:23596, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:58052, ChEBI:CHEBI:58885; EC=1.1.1.22;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000124};
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family. {ECO:0000256|PIRNR:PIRNR000124}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP002117; ADN35783.1; -; Genomic_DNA.
DR   RefSeq; WP_013328961.1; NC_014507.1.
DR   STRING; 679926.Mpet_1016; -.
DR   EnsemblBacteria; ADN35783; ADN35783; Mpet_1016.
DR   GeneID; 9743480; -.
DR   KEGG; mpi:Mpet_1016; -.
DR   eggNOG; arCOG00253; Archaea.
DR   eggNOG; COG1004; LUCA.
DR   HOGENOM; HOG000153773; -.
DR   KO; K00012; -.
DR   OMA; LCTEWDE; -.
DR   OrthoDB; 47911at2157; -.
DR   BioCyc; MPET679926:G1GN5-1043-MONOMER; -.
DR   Proteomes; UP000006565; Chromosome.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0003979; F:UDP-glucose 6-dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:InterPro.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR017476; UDP-Glc/GDP-Man.
DR   InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR   InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR   InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR   InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR   InterPro; IPR028357; UDPglc_DH_bac.
DR   Pfam; PF00984; UDPG_MGDP_dh; 1.
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR   PIRSF; PIRSF500134; UDPglc_DH_bac; 1.
DR   PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1.
DR   SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52413; SSF52413; 1.
DR   TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE   3: Inferred from homology;
DR   PRODOM; E1RK60.
DR   SWISS-2DPAGE; E1RK60.
KW   NAD {ECO:0000256|PIRNR:PIRNR000124, ECO:0000256|PIRSR:PIRSR500134-3};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000124,
KW   ECO:0000313|EMBL:ADN35783.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006565}.
FT   DOMAIN          315..414
FT                   /note="UDPG_MGDP_dh_C"
FT                   /evidence="ECO:0000259|SMART:SM00984"
FT   ACT_SITE        262
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-1"
FT   BINDING         30
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         35
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         84
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         122
FT                   /note="NAD; via amide nitrogen"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         157
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         265
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
FT   BINDING         329
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR500134-3"
SQ   SEQUENCE   422 AA;  45638 MW;  A9B444F9219FB3C3 CRC64;
     MKISVVGGGY VGLITGACLA EKGHHVTIID IDKEKINAIN SARPPIYEKG LEELLARHSG
     KNLLASTGYE SVRDSEISII CVGTPMGEDG SADLKYIVSA AESIGRELKN SGVYHVVTVK
     STVPPGTTSG IVVPAVMSAS GNNPNIGFAM NPEFLREGIA VDDFMNPDRV VIGSDDKKSG
     DLVESLYSDF NIRILRTGLV SAEMIKYASN SFLATKISFS NEVGNICKSF GIDVYEVMEG
     VGLDHRISPY FLNAGCGFGG SCFPKDVSAL IHLAKSKGVD PVLLRSVLKV NEVQPLLIIE
     ILKKRLGDLN GKRIAVLGLA FKGDTDDIRD SRAVPAIERL IMEGVDVHAF DPMANENMKA
     VFPEITYHDS AAGALENADG CLVMTEWPEF SRLDKEFDKM KKRVIIEGRR ILSIPDAEGI
     CW
//

If you have problems or comments...

PBIL Back to PBIL home page