(data stored in SCRATCH zone)

SWISSPROT: E3J7D2_FRAIE

ID   E3J7D2_FRAIE            Unreviewed;       794 AA.
AC   E3J7D2;
DT   11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT   11-JAN-2011, sequence version 1.
DT   16-JAN-2019, entry version 48.
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000256|HAMAP-Rule:MF_01129};
DE            EC=7.1.3.1 {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE            Short=H(+)-PPase {ECO:0000256|HAMAP-Rule:MF_01129};
GN   Name=hppA {ECO:0000256|HAMAP-Rule:MF_01129};
GN   OrderedLocusNames=FraEuI1c_0319 {ECO:0000313|EMBL:ADP78405.1};
OS   Frankia inefficax (strain DSM 45817 / CECT 9037 / EuI1c).
OC   Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia.
OX   NCBI_TaxID=298654 {ECO:0000313|EMBL:ADP78405.1, ECO:0000313|Proteomes:UP000002484};
RN   [1] {ECO:0000313|EMBL:ADP78405.1, ECO:0000313|Proteomes:UP000002484}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 45817 / CECT 9037 / EuI1c
RC   {ECO:0000313|Proteomes:UP000002484};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA   Beauchemin N., Sen A., Sur S.A., Gtari M., Wall L., Tisa L., Woyke T.;
RT   "Complete sequence of Frankia sp. EuI1c.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force. {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01129};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01129};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01129}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase
CC       (TC 3.A.10) family. K(+)-insensitive subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01129}.
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DR   EMBL; CP002299; ADP78405.1; -; Genomic_DNA.
DR   STRING; 298654.FraEuI1c_0319; -.
DR   EnsemblBacteria; ADP78405; ADP78405; FraEuI1c_0319.
DR   KEGG; fri:FraEuI1c_0319; -.
DR   eggNOG; ENOG4105EAY; Bacteria.
DR   eggNOG; COG3808; LUCA.
DR   HOGENOM; HOG000007098; -.
DR   KO; K15987; -.
DR   OMA; MATTAMQ; -.
DR   Proteomes; UP000002484; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009678; F:hydrogen-translocating pyrophosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01129; PPase_energized_pump; 1.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
DR   PIRSF; PIRSF001265; H+-PPase; 1.
DR   TIGRFAMs; TIGR01104; V_PPase; 1.
PE   3: Inferred from homology;
DR   PRODOM; E3J7D2.
DR   SWISS-2DPAGE; E3J7D2.
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002484};
KW   Hydrogen ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Hydrolase {ECO:0000313|EMBL:ADP78405.1};
KW   Ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002484};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01129}.
FT   TRANSMEM     22     43       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     77     94       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    106    126       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    147    174       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    180    198       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    251    269       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    275    296       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    308    332       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    352    375       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    409    427       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    433    453       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    495    516       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    548    570       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    615    635       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    641    658       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    733    753       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   SITE        494    494       Determinant of potassium independence.
FT                                {ECO:0000256|HAMAP-Rule:MF_01129}.
SQ   SEQUENCE   794 AA;  79830 MW;  47C0F254F878F2C0 CRC64;
     MPPIQAAESP AGTGIDLTGG QLGLVAGVAL IAALALLVAG YLVREVLAAS QGGPKMIEVG
     KAVQEGAAAY LSRQFKTLAA FVVVIPFVLL ALPADDLGAR IGRSAFFVVG AVFSALVGFV
     GMSLATRANT RTAAAANERG EKAALRLAFR TGGVVGMFTV GLGLLGAAVV VLIFKHTAPQ
     VLEGFGFGAA LLAMFMRVGG GIFTKAADVG ADLVGKVEKG IPEDDPRNAA TIADNVGDNV
     GDCAGMAADL FESYAVTLVA ALILGIKAFG SHGLVFPLLI PAIGVITAVI GIFAVAPRSG
     DRDGMAAINR GFFISAVISA IGVVVVALVY LPGSFKGFHD LAGGVPKGDP RVIAISACLI
     GIVLAAAIQL LTGYFTETKR KPVRSVVDAS TTGPATNILA GIGVGLESAV YSAVLISAAV
     FGAYLLGSGS ITVALFAVAM AGTGLLTTVG VIISMDTFGP VSDNAQGIAE MSGEVTEEGA
     TILTSLDAVG NTTKAITKGI AIATAVFAAT ALFGSFTDTI TEALAKAGVN PSTTRGTIGG
     LNVAYPDALV GLILGAAVVF LFSGLAINAV GRAAGRVVWE VRKQFQDHPG IMEGTEKPDY
     GAVVDICTRD SLRELVTPGT LAVMAPIAVG FGLGYPPLGC YLAGAIAGGV LMAVFLANSG
     GAWDNAKKLV EDGMHGGKGS DVHAATVIGD TVGDPFKDTA GPAINPLIKV MNLVSLLIAP
     TVVKFSVGQD KNTWLRVLIA IVALAVIATV ITISKRRGSA LDAVPGDSGT AVSSNVVVAT
     AAGQPATAPD QVNV
//

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