(data stored in SCRATCH zone)

SWISSPROT: E6S641_INTC7

ID   E6S641_INTC7            Unreviewed;       437 AA.
AC   E6S641;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   08-MAY-2019, entry version 35.
DE   RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444};
DE            EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444};
DE   AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444};
GN   OrderedLocusNames=Intca_0224 {ECO:0000313|EMBL:ADU46781.1};
OS   Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 /
OS   NBRC 12989 / 7 KIP).
OC   Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
OC   Intrasporangium.
OX   NCBI_TaxID=710696 {ECO:0000313|EMBL:ADU46781.1, ECO:0000313|Proteomes:UP000008914};
RN   [1] {ECO:0000313|EMBL:ADU46781.1, ECO:0000313|Proteomes:UP000008914}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP
RC   {ECO:0000313|Proteomes:UP000008914};
RX   PubMed=21304734; DOI=10.4056/sigs.1263355;
RA   Del Rio T.G., Chertkov O., Yasawong M., Lucas S., Deshpande S.,
RA   Cheng J.F., Detter C., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA   Rohde M., Pukall R., Sikorski J., Goker M., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA   Lapidus A.;
RT   "Complete genome sequence of Intrasporangium calvum type strain (7
RT   KIP).";
RL   Stand. Genomic Sci. 3:294-303(2010).
CC   -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to
CC       phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-phenylacetate + ATP + CoA = AMP + diphosphate +
CC         phenylacetyl-CoA; Xref=Rhea:RHEA:20956, ChEBI:CHEBI:18401,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57390, ChEBI:CHEBI:456215; EC=6.2.1.30;
CC         Evidence={ECO:0000256|PIRNR:PIRNR006444};
CC   -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC       {ECO:0000256|PIRNR:PIRNR006444}.
CC   -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family.
CC       {ECO:0000256|PIRNR:PIRNR006444}.
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DR   EMBL; CP002343; ADU46781.1; -; Genomic_DNA.
DR   RefSeq; WP_013491103.1; NC_014830.1.
DR   STRING; 710696.Intca_0224; -.
DR   EnsemblBacteria; ADU46781; ADU46781; Intca_0224.
DR   KEGG; ica:Intca_0224; -.
DR   eggNOG; ENOG4105DZS; Bacteria.
DR   eggNOG; COG1541; LUCA.
DR   HOGENOM; HOG000197614; -.
DR   KO; K01912; -.
DR   OrthoDB; 839891at2; -.
DR   BioCyc; ICAL710696:GH9U-228-MONOMER; -.
DR   UniPathway; UPA00930; -.
DR   Proteomes; UP000008914; Chromosome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0010124; P:phenylacetate catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR028154; AMP-dep_Lig_C.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; AMP-dep_Synthh-like_sf.
DR   InterPro; IPR011880; PA_CoA_ligase.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF14535; AMP-binding_C_2; 1.
DR   PIRSF; PIRSF006444; PaaK; 1.
DR   TIGRFAMs; TIGR02155; PA_CoA_ligase; 1.
PE   3: Inferred from homology;
DR   PRODOM; E6S641.
DR   SWISS-2DPAGE; E6S641.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008914};
KW   Ligase {ECO:0000256|PIRNR:PIRNR006444, ECO:0000313|EMBL:ADU46781.1};
KW   Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008914}.
FT   DOMAIN       94    294       AMP-binding. {ECO:0000259|Pfam:PF00501}.
FT   DOMAIN      341    434       AMP-binding_C_2. {ECO:0000259|Pfam:
FT                                PF14535}.
SQ   SEQUENCE   437 AA;  48224 MW;  CFA11218F6D39EB2 CRC64;
     MTTESPAPAL PDHEIPTLSV DELRHLQTER LRATISAAYA NVPHYTAAFD AKGLTPDDIR
     SLDDLRRLPF LAKADLRDYY PFGMFAVPRT EVVRIHASSG TTGRPTVVGY TKQDIDTWAD
     LMARSIRFAG GGPGDIIHVA YGYGLFTGGL GAHYGAERLG GTVVPVSGGM TERQVQLIRD
     FGATVILVTP SYFLSLIDQM ESEGIDPRST SLRVGIFGAE PWTEAMRREI EERTGMDAVD
     IYGLSEVMGP GVAQEAGTTK DGLHLWEDHF YPEIIDPVTE EVLPDGEFGE LVLTSLTRQA
     MPVIRYRTRD LTRLLPGTAC PSMRRMEKVT GRSDDMMIIR GVNVFPTQIE EFVLAEPRLS
     PYFQCVLRQP GRMVELTVLV EPVESLGDAD ADAIARHLQH EIKTNIGTSC RVEVRAPGSI
     ERSIGKARRI VDERPKG
//

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