(data stored in ACNUC7421 zone)

SWISSPROT: ENCB_ASPFU

ID   ENCB_ASPFU              Reviewed;         315 AA.
AC   Q4W945;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   11-DEC-2019, entry version 70.
DE   RecName: Full=Atrochrysone carboxyl ACP thioesterase {ECO:0000250|UniProtKB:Q0CCY4};
DE            Short=ACTE {ECO:0000250|UniProtKB:Q0CCY4};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:Q0CCY4};
DE   AltName: Full=Endocrocin synthesis protein B {ECO:0000303|PubMed:22492455};
GN   Name=encB {ECO:0000303|PubMed:22492455}; ORFNames=AFUA_4G00220;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=20379952; DOI=10.1055/s-0030-1249779;
RA   Gautam R., Karkhile K.V., Bhutani K.K., Jachak S.M.;
RT   "Anti-inflammatory, cyclooxygenase (COX)-2, COX-1 inhibitory, and free
RT   radical scavenging effects of Rumex nepalensis.";
RL   Planta Med. 76:1564-1569(2010).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=22492455; DOI=10.1128/aem.07710-11;
RA   Lim F.Y., Hou Y., Chen Y., Oh J.H., Lee I., Bugni T.S., Keller N.P.;
RT   "Genome-based cluster deletion reveals an endocrocin biosynthetic pathway
RT   in Aspergillus fumigatus.";
RL   Appl. Environ. Microbiol. 78:4117-4125(2012).
RN   [4]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=23592999; DOI=10.1371/journal.ppat.1003289;
RA   Berthier E., Lim F.Y., Deng Q., Guo C.J., Kontoyiannis D.P., Wang C.C.,
RA   Rindy J., Beebe D.J., Huttenlocher A., Keller N.P.;
RT   "Low-volume toolbox for the discovery of immunosuppressive fungal secondary
RT   metabolites.";
RL   PLoS Pathog. 9:E1003289-E1003289(2013).
RN   [5]
RP   INDUCTION.
RX   PubMed=26242966; DOI=10.1111/1462-2920.13007;
RA   Throckmorton K., Lim F.Y., Kontoyiannis D.P., Zheng W., Keller N.P.;
RT   "Redundant synthesis of a conidial polyketide by two distinct secondary
RT   metabolite clusters in Aspergillus fumigatus.";
RL   Environ. Microbiol. 18:246-259(2016).
CC   -!- FUNCTION: Atrochrysone carboxyl ACP thioesterase; part of the gene
CC       cluster that mediates the biosynthesis of endocrocin, a simple
CC       anthraquinone interesting for many biotechnological applications
CC       (PubMed:22492455, PubMed:23592999). The pathway begins with the
CC       synthesis of atrochrysone thioester by the polyketide synthase (PKS)
CC       encA (PubMed:22492455). The atrochrysone carboxyl ACP thioesterase encB
CC       then breaks the thioester bond and releases the atrochrysone carboxylic
CC       acid from encA (PubMed:22492455). The atrochrysone carboxylic acid is
CC       then converted to endocrocin anthrone which is further oxidized into
CC       endocrocin by the anthrone oxygenase encC (PubMed:22492455). The exact
CC       function of encD has not been identified yet, but it negatively
CC       regulates endocrocin production, likely through the modification of
CC       endocrocin itself (PubMed:22492455). {ECO:0000269|PubMed:22492455,
CC       ECO:0000269|PubMed:23592999}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:Q988B9};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250|UniProtKB:Q988B9};
CC   -!- TISSUE SPECIFICITY: Endocrocin is specifically produced in conidia.
CC       {ECO:0000305|PubMed:23592999}.
CC   -!- INDUCTION: Expression is positively regulated by the transcription
CC       factors brlA and laeA (PubMed:26242966). {ECO:0000269|PubMed:26242966}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of endocrocin
CC       (PubMed:22492455). {ECO:0000269|PubMed:22492455}.
CC   -!- BIOTECHNOLOGY: Endocrocin and related anthraquinones compounds have
CC       interesting activities for medicinal uses, including anti-inflammatory
CC       activity (PubMed:20379952). {ECO:0000269|PubMed:20379952}.
CC   -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC       {ECO:0000305}.
DR   EMBL; AAHF01000017; EAL84396.1; -; Genomic_DNA.
DR   RefSeq; XP_746434.1; XM_741341.1.
DR   SMR; Q4W945; -.
DR   STRING; 746128.CADAFUBP00009781; -.
DR   EnsemblFungi; EAL84396; EAL84396; AFUA_4G00220.
DR   GeneID; 3503738; -.
DR   KEGG; afm:AFUA_4G00220; -.
DR   EuPathDB; FungiDB:Afu4g00220; -.
DR   HOGENOM; HOG000246879; -.
DR   InParanoid; Q4W945; -.
DR   OMA; NGQIFQT; -.
DR   OrthoDB; 576967at2759; -.
DR   Proteomes; UP000002530; Chromosome 4.
DR   Proteomes; UP000002530; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:1900602; P:endocrocin biosynthetic process; IMP:AspGD.
DR   Gene3D; 3.60.15.10; -; 1.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   Pfam; PF00753; Lactamase_B; 1.
DR   SMART; SM00849; Lactamase_B; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q4W945.
DR   SWISS-2DPAGE; Q4W945.
KW   Hydrolase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..315
FT                   /note="Atrochrysone carboxyl ACP thioesterase"
FT                   /id="PRO_0000437053"
FT   ACT_SITE        99
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000255"
FT   METAL           95
FT                   /note="Zinc 1; via tele nitrogen; catalytic"
FT                   /evidence="ECO:0000250|UniProtKB:Q988B9"
FT   METAL           97
FT                   /note="Zinc 1; via pros nitrogen; catalytic"
FT                   /evidence="ECO:0000250|UniProtKB:Q988B9"
FT   METAL           99
FT                   /note="Zinc 2; catalytic"
FT                   /evidence="ECO:0000250|UniProtKB:Q988B9"
FT   METAL           100
FT                   /note="Zinc 2; via tele nitrogen; catalytic"
FT                   /evidence="ECO:0000250|UniProtKB:Q988B9"
SQ   SEQUENCE   315 AA;  35346 MW;  73235EAF8EB23588 CRC64;
     MDQYSNLFAF EDYLGAQARS IPDLPEVDVL SPRVVRVLGG NPGQMQLQGT NTYILGTGAE
     RLLIDSGQGR ARWEQLMASL AAEHKFRIST VLLTHWHLDH TGGVPHLFRI FPELRGANAI
     YKYHPDPSQQ AIVDGQVFSV EGATVRAVFT PGHSTDHMCF LLQEEEAIFT GDTVLGHGTT
     GVEDLEEYMQ SLRKIQSLGC RIGYPGHGAV IENMQQKVQQ EIDRKQRRER QVLLALQNIQ
     REKRTVGDAN GAATQAELIE AIFGRLPADV ADRFFAPYMK DILMKMARDK QVGFRFKGGQ
     KHWFANVSQE NPVCR
//

If you have problems or comments...

PBIL Back to PBIL home page