(data stored in ACNUC7421 zone)

SWISSPROT: DXO_ASPFU

ID   DXO_ASPFU               Reviewed;         365 AA.
AC   Q4WDK5;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   11-DEC-2019, entry version 81.
DE   RecName: Full=Decapping nuclease rai1;
DE            EC=3.6.1.-;
GN   Name=rai1; ORFNames=AFUA_6G05060;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: Ribonuclease that specifically degrades pre-mRNAs with a
CC       defective 5' end cap and is part of a pre-mRNA capping quality control.
CC       Has decapping and pyrophosphohydrolase activities. Has decapping
CC       activity toward incomplete 5' end cap mRNAs such as unmethylated 5'
CC       end-capped RNA to release GpppN and 5' end monophosphate RNA. Also
CC       possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'
CC       end triphosphate to release pyrophosphates (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Divalent metal cation. {ECO:0000250};
CC   -!- SUBUNIT: Interacts with RAT1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DXO/Dom3Z family. {ECO:0000305}.
DR   EMBL; AAHF01000012; EAL85533.1; -; Genomic_DNA.
DR   RefSeq; XP_747571.1; XM_742478.1.
DR   SMR; Q4WDK5; -.
DR   STRING; 746128.CADAFUBP00009059; -.
DR   EnsemblFungi; EAL85533; EAL85533; AFUA_6G05060.
DR   GeneID; 3505338; -.
DR   KEGG; afm:AFUA_6G05060; -.
DR   EuPathDB; FungiDB:Afu6g05060; -.
DR   HOGENOM; HOG000200647; -.
DR   InParanoid; Q4WDK5; -.
DR   KO; K14845; -.
DR   OMA; VVTWRGH; -.
DR   OrthoDB; 1034620at2759; -.
DR   Proteomes; UP000002530; Chromosome 6.
DR   Proteomes; UP000002530; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR013961; RAI1.
DR   InterPro; IPR039039; RAI1-like_fam.
DR   PANTHER; PTHR12395; PTHR12395; 1.
DR   Pfam; PF08652; RAI1; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q4WDK5.
DR   SWISS-2DPAGE; Q4WDK5.
KW   Hydrolase; Metal-binding; mRNA processing; Nuclease; Nucleotide-binding;
KW   Nucleus; Reference proteome; RNA-binding; rRNA processing; Transcription;
KW   Transcription regulation; Transcription termination.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..365
FT                   /note="Decapping nuclease rai1"
FT                   /id="PRO_0000249827"
FT   METAL           165
FT                   /note="Divalent metal cation"
FT                   /evidence="ECO:0000250"
FT   METAL           216
FT                   /note="Divalent metal cation"
FT                   /evidence="ECO:0000250"
FT   METAL           234
FT                   /note="Divalent metal cation"
FT                   /evidence="ECO:0000250"
FT   METAL           235
FT                   /note="Divalent metal cation; via carbonyl oxygen"
FT                   /evidence="ECO:0000250"
FT   BINDING         73
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         101
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         197
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         214
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         260
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   365 AA;  42031 MW;  A7E550C4880C910B CRC64;
     MDGGTFDIQP IGRFYGSNTT IRRPREITCF SYDAEHKFHL GDSSLRYYYT PRLPADLNRG
     FDTFQKLDDT ADEHLDALLE TIMALEKETG KRCEADIITW RGMMTKILTA PFDNLNGFEM
     NATCFQVGGE NNLYKIQQKQ IQENQRMPPG MASQDLMAYW GYKFETLCLL QQPWDPTPRA
     EIESREDLVV NNNAQYCSVV RTGIGSTRLI IGGEVDAVWD CKPDRKEDPI NWVELKTSAE
     IRNDRDMIKY ERKLLKFWAQ SFLLGVPKII VGFRDNHGIV HRLEELETAS IPNKVKKLGR
     GTWDGNICIN FAAAFLEWLK STIKEGGTWR IRKLEKSSLI QVFKIEETGT GDIISRSFLD
     WRSRS
//

If you have problems or comments...

PBIL Back to PBIL home page