(data stored in SCRATCH zone)

EMBL: K02174

ID   K02174; SV 1; linear; genomic DNA; STD; PRO; 476 BP.
XX
AC   K02174;
XX
DT   07-NOV-1985 (Rel. 07, Created)
DT   05-JUL-1999 (Rel. 60, Last updated, Version 5)
XX
DE   B.subtilis P43 fragment containing two overlapping promoters.
XX
KW   promoter region.
XX
OS   Bacillus subtilis
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-476
RX   PUBMED; 6330116.
RA   Wang P.-Z., Doi R.H.;
RT   "Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma
RT   37 RNA polymerase holoenzymes during growth and stationary phases";
RL   J Biol Chem 259(13):8619-8625(1984).
XX
DR   MD5; ec71a00d60f7a02dfafe6dab22517554.
DR   EuropePMC; PMC5835740; 29330178.
XX
CC   This 476 bp fragment DNA can express the chloramphenicol
CC   acetyltransferase gene in pGR71 efficiently. Enzymological and DNA
CC   sequencing studies have revealed that this fragment actually
CC   comprises two overlapping promoters, one recognized by the major
CC   sigma-55 RNA polymerase (-35 region, bases 372-377; -10 region,
CC   bases 395-400) and the other by the minor sigma-37 RNA polymerase
CC   (-35 region, bases 362-370; -10 region, bases 386-395).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..476
FT                   /organism="Bacillus subtilis"
FT                   /strain="W168"
FT                   /sub_strain="PY79"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1423"
FT   mRNA            405..>476
FT                   /note="P43 mRNA"
FT   CDS_pept        446..>476
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="P43"
FT                   /db_xref="GOA:P19079"
FT                   /db_xref="InterPro:IPR002125"
FT                   /db_xref="InterPro:IPR006262"
FT                   /db_xref="InterPro:IPR016192"
FT                   /db_xref="InterPro:IPR016193"
FT                   /db_xref="PDB:1JTK"
FT                   /db_xref="PDB:1UWZ"
FT                   /db_xref="PDB:1UX0"
FT                   /db_xref="PDB:1UX1"
FT                   /db_xref="UniProtKB/Swiss-Prot:P19079"
FT                   /protein_id="AAB05347.1"
FT                   /translation="MNRQELITEA"
XX
SQ   Sequence 476 BP; 130 A; 89 C; 111 G; 146 T; 0 other;
     agcttcgtgc atgcaggccg gggcatatgg gaaacagcgc ggacgcagcg gaatttccaa        60
     tttcatgccg cagccgcctg cgctgttctc atttgcggct tccttgtaga gctcagcatt       120
     attgagtgga tgattatatt ccttttgata ggtggtatgt tttcgcttga acttttaaat       180
     acagccattg aacatacggt tgatttaata actgacaaac atcaccctct tgctaaagcg       240
     gccaaggacg ctgccgccgg ggctgtttgc gtttttgccg tgatttcgtg tatcattggt       300
     ttacttattt ttttgccaaa gctgtaatgg ctgaaaattc ttacatttat tttacatttt       360
     tagaaatggg cgtgaaaaaa agcgcgcgat tatgtaaaat ataaagtgat agcggtacca       420
     ttataggtaa gagaggaatg tacacatgaa cagacaagaa ttaataacag aagctt           476
//

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