(data stored in ACNUC1829 zone)

SWISSPROT: TSN4_HUMAN

ID   TSN4_HUMAN              Reviewed;         238 AA.
AC   O14817; Q6IAP6;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   30-AUG-2017, entry version 135.
DE   RecName: Full=Tetraspanin-4;
DE            Short=Tspan-4;
DE   AltName: Full=Novel antigen 2;
DE            Short=NAG-2;
DE   AltName: Full=Transmembrane 4 superfamily member 7;
GN   Name=TSPAN4; Synonyms=NAG2, TM4SF7;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9360996; DOI=10.1074/jbc.272.46.29181;
RA   Tachibana I., Bodorova J., Berditchevski F., Zutter M.M., Hemler M.E.;
RT   "NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that
RT   complexes with integrins and other TM4SF proteins.";
RL   J. Biol. Chem. 272:29181-29189(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9714763; DOI=10.1016/S0167-4781(98)00087-6;
RA   Todd S.C., Doctor V.S., Levy S.;
RT   "Sequences and expression of six new members of the tetraspanin/TM4SF
RT   family.";
RL   Biochim. Biophys. Acta 1399:101-104(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Teratocarcinoma;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-161.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of
RT   multiple enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
RN   [9]
RP   VARIANT [LARGE SCALE ANALYSIS] MET-67.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S.,
RA   Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J.,
RA   Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C.,
RA   Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N.,
RA   Vogelstein B., Kinzler K.W., Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal
RT   cancers.";
RL   Science 314:268-274(2006).
CC   -!- SUBUNIT: Forms a complex with integrins.
CC   -!- INTERACTION:
CC       Q8NEC5:CATSPER1; NbExp=4; IntAct=EBI-8652667, EBI-744545;
CC       A8MQ03:CYSRT1; NbExp=4; IntAct=EBI-8652667, EBI-3867333;
CC       O43559:FRS3; NbExp=4; IntAct=EBI-8652667, EBI-725515;
CC       P49639:HOXA1; NbExp=4; IntAct=EBI-8652667, EBI-740785;
CC       Q5TA76:LCE3A; NbExp=4; IntAct=EBI-8652667, EBI-9394625;
CC       Q7Z3S9:NOTCH2NL; NbExp=5; IntAct=EBI-8652667, EBI-945833;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in multiple tissues but is absent in
CC       brain, lymphoid cells, and platelets.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family.
CC       {ECO:0000305}.
DR   EMBL; AF022813; AAC51864.1; -; mRNA.
DR   EMBL; AF054841; AAC69717.1; -; mRNA.
DR   EMBL; AK291089; BAF83778.1; -; mRNA.
DR   EMBL; BT006776; AAP35422.1; -; mRNA.
DR   EMBL; CR457108; CAG33389.1; -; mRNA.
DR   EMBL; CH471158; EAX02409.1; -; Genomic_DNA.
DR   EMBL; BC000389; AAH00389.1; -; mRNA.
DR   EMBL; BC019314; AAH19314.1; -; mRNA.
DR   EMBL; BC093031; AAH93031.1; -; mRNA.
DR   CCDS; CCDS7721.1; -.
DR   PIR; A59265; A59265.
DR   RefSeq; NP_001020405.1; NM_001025234.1.
DR   RefSeq; NP_001020406.1; NM_001025235.1.
DR   RefSeq; NP_001020407.1; NM_001025236.1.
DR   RefSeq; NP_001020408.1; NM_001025237.1.
DR   RefSeq; NP_001020409.1; NM_001025238.1.
DR   RefSeq; NP_001020410.1; NM_001025239.1.
DR   RefSeq; NP_003262.1; NM_003271.4.
DR   RefSeq; XP_005253159.1; XM_005253102.1.
DR   UniGene; Hs.654836; -.
DR   ProteinModelPortal; O14817; -.
DR   BioGrid; 112961; 7.
DR   IntAct; O14817; 35.
DR   STRING; 9606.ENSP00000380552; -.
DR   SwissPalm; O14817; -.
DR   BioMuta; TSPAN4; -.
DR   EPD; O14817; -.
DR   MaxQB; O14817; -.
DR   PaxDb; O14817; -.
DR   PeptideAtlas; O14817; -.
DR   PRIDE; O14817; -.
DR   DNASU; 7106; -.
DR   Ensembl; ENST00000397397; ENSP00000380552; ENSG00000214063.
DR   Ensembl; ENST00000397404; ENSP00000380553; ENSG00000214063.
DR   Ensembl; ENST00000397406; ENSP00000380554; ENSG00000214063.
DR   Ensembl; ENST00000397408; ENSP00000380555; ENSG00000214063.
DR   Ensembl; ENST00000397411; ENSP00000380558; ENSG00000214063.
DR   Ensembl; ENST00000409543; ENSP00000386513; ENSG00000214063.
DR   GeneID; 7106; -.
DR   KEGG; hsa:7106; -.
DR   UCSC; uc001lsd.2; human.
DR   CTD; 7106; -.
DR   DisGeNET; 7106; -.
DR   GeneCards; TSPAN4; -.
DR   HGNC; HGNC:11859; TSPAN4.
DR   MIM; 602644; gene.
DR   neXtProt; NX_O14817; -.
DR   OpenTargets; ENSG00000214063; -.
DR   PharmGKB; PA36560; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   eggNOG; ENOG4111IRY; LUCA.
DR   GeneTree; ENSGT00880000137858; -.
DR   HOGENOM; HOG000230651; -.
DR   HOVERGEN; HBG002324; -.
DR   InParanoid; O14817; -.
DR   KO; K17294; -.
DR   PhylomeDB; O14817; -.
DR   TreeFam; TF352892; -.
DR   ChiTaRS; TSPAN4; human.
DR   GeneWiki; TSPAN4; -.
DR   GenomeRNAi; 7106; -.
DR   PRO; PR:O14817; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   Bgee; ENSG00000214063; -.
DR   CleanEx; HS_TSPAN4; -.
DR   ExpressionAtlas; O14817; baseline and differential.
DR   Genevisible; O14817; HS.
DR   GO; GO:0005925; C:focal adhesion; IDA:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR   GO; GO:0003823; F:antigen binding; IDA:UniProtKB.
DR   GO; GO:0005178; F:integrin binding; IPI:UniProtKB.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0006461; P:protein complex assembly; IDA:UniProtKB.
DR   Gene3D; 1.10.1450.10; -; 1.
DR   InterPro; IPR000301; Tetraspanin.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2.
DR   Pfam; PF00335; Tetraspannin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; SSF48652; 1.
DR   PROSITE; PS00421; TM4_1; 1.
PE   1: Evidence at protein level;
DR   PRODOM; O14817.
DR   SWISS-2DPAGE; O14817.
KW   Complete proteome; Glycoprotein; Membrane; Polymorphism;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN         1    238       Tetraspanin-4.
FT                                /FTId=PRO_0000219241.
FT   TOPO_DOM      1     13       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     14     34       Helical. {ECO:0000255}.
FT   TOPO_DOM     35     55       Extracellular. {ECO:0000255}.
FT   TRANSMEM     56     76       Helical. {ECO:0000255}.
FT   TOPO_DOM     77     85       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     86    106       Helical. {ECO:0000255}.
FT   TOPO_DOM    107    201       Extracellular. {ECO:0000255}.
FT   TRANSMEM    202    222       Helical. {ECO:0000255}.
FT   TOPO_DOM    223    238       Cytoplasmic. {ECO:0000255}.
FT   CARBOHYD    152    152       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    161    161       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000269|PubMed:19159218}.
FT   VARIANT      67     67       I -> M (in a breast cancer sample;
FT                                somatic mutation).
FT                                {ECO:0000269|PubMed:16959974}.
FT                                /FTId=VAR_036616.
SQ   SEQUENCE   238 AA;  26118 MW;  A6B9A5633065A492 CRC64;
     MARACLQAVK YLMFAFNLLF WLGGCGVLGV GIWLAATQGS FATLSSSFPS LSAANLLIIT
     GAFVMAIGFV GCLGAIKENK CLLLTFFLLL LLVFLLEATI AILFFAYTDK IDRYAQQDLK
     KGLHLYGTQG NVGLTNAWSI IQTDFRCCGV SNYTDWFEVY NATRVPDSCC LEFSESCGLH
     APGTWWKAPC YETVKVWLQE NLLAVGIFGL CTALVQILGL TFAMTMYCQV VKADTYCA
//

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