(data stored in SCRATCH zone)

SWISSPROT: SRPB_YEAST

ID   SRPB_YEAST              Reviewed;         244 AA.
AC   P36057; D6VX44;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   05-JUL-2017, entry version 156.
DE   RecName: Full=Signal recognition particle receptor subunit beta;
DE            Short=SR-beta;
GN   Name=SRP102; OrderedLocusNames=YKL154W; ORFNames=YKL609;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091859; DOI=10.1002/yea.320100005;
RA   Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.;
RT   "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP
RT   loci of chromosome XI of Saccharomyces cerevisiae.";
RL   Yeast 10:S35-S40(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
RA   Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
RA   Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
RA   Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-244 IN COMPLEX WITH GTP
RP   AND SRP101, AND SUBUNIT.
RX   PubMed=12654246; DOI=10.1016/S0092-8674(03)00161-2;
RA   Schwartz T., Blobel G.;
RT   "Structural basis for the function of the beta subunit of the
RT   eukaryotic signal recognition particle receptor.";
RL   Cell 112:793-803(2003).
CC   -!- FUNCTION: Component of the SRP (signal recognition particle)
CC       receptor. Ensures, in conjunction with the signal recognition
CC       particle, the correct targeting of the nascent secretory proteins
CC       to the endoplasmic reticulum membrane system. Has GTPase activity.
CC       May mediate the membrane association of SRPR.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000269|PubMed:12654246}.
CC   -!- INTERACTION:
CC       P32916:SRP101; NbExp=5; IntAct=EBI-18091, EBI-18098;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 8250 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SRP receptor beta subunit family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA81499.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; Z26877; CAA81499.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Z28154; CAA81995.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09010.1; -; Genomic_DNA.
DR   PIR; S37984; S37984.
DR   RefSeq; NP_012768.1; NM_001179720.1.
DR   PDB; 1NRJ; X-ray; 1.70 A; B=31-244.
DR   PDB; 2GED; X-ray; 2.20 A; A/B=36-183.
DR   PDBsum; 1NRJ; -.
DR   PDBsum; 2GED; -.
DR   ProteinModelPortal; P36057; -.
DR   SMR; P36057; -.
DR   BioGrid; 33983; 422.
DR   DIP; DIP-4753N; -.
DR   IntAct; P36057; 15.
DR   MINT; MINT-365016; -.
DR   STRING; 4932.YKL154W; -.
DR   TCDB; 3.A.5.8.1; the general secretory pathway (sec) family.
DR   MaxQB; P36057; -.
DR   PRIDE; P36057; -.
DR   EnsemblFungi; YKL154W; YKL154W; YKL154W.
DR   GeneID; 853702; -.
DR   KEGG; sce:YKL154W; -.
DR   EuPathDB; FungiDB:YKL154W; -.
DR   SGD; S000001637; SRP102.
DR   GeneTree; ENSGT00510000048347; -.
DR   HOGENOM; HOG000248720; -.
DR   InParanoid; P36057; -.
DR   KO; K12272; -.
DR   OMA; NKQDQTT; -.
DR   OrthoDB; EOG092C5BU6; -.
DR   BioCyc; YEAST:G3O-31924-MONOMER; -.
DR   EvolutionaryTrace; P36057; -.
DR   PRO; PR:P36057; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR   GO; GO:0005785; C:signal recognition particle receptor complex; IPI:SGD.
DR   GO; GO:0005525; F:GTP binding; IPI:SGD.
DR   GO; GO:0003924; F:GTPase activity; ISS:SGD.
DR   GO; GO:0005047; F:signal recognition particle binding; IMP:SGD.
DR   GO; GO:0045047; P:protein targeting to ER; IMP:SGD.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IC:SGD.
DR   CDD; cd04105; SR_beta; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR019009; SRP_receptor_beta_su.
DR   Pfam; PF09439; SRPRB; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P36057.
DR   SWISS-2DPAGE; P36057.
KW   3D-structure; Complete proteome; Endoplasmic reticulum; GTP-binding;
KW   Membrane; Nucleotide-binding; Receptor; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN         1    244       Signal recognition particle receptor
FT                                subunit beta.
FT                                /FTId=PRO_0000101230.
FT   TRANSMEM      7     23       Helical. {ECO:0000255}.
FT   NP_BIND      45     53       GTP. {ECO:0000269|PubMed:12654246}.
FT   NP_BIND      66     69       GTP. {ECO:0000269|PubMed:12654246}.
FT   NP_BIND     154    157       GTP. {ECO:0000269|PubMed:12654246}.
FT   BINDING      90     90       GTP; via amide nitrogen.
FT                                {ECO:0000269|PubMed:12654246}.
FT   STRAND       40     44       {ECO:0000244|PDB:1NRJ}.
FT   HELIX        51     60       {ECO:0000244|PDB:1NRJ}.
FT   STRAND       73     75       {ECO:0000244|PDB:1NRJ}.
FT   HELIX        78     80       {ECO:0000244|PDB:1NRJ}.
FT   STRAND       84     87       {ECO:0000244|PDB:1NRJ}.
FT   HELIX        92     95       {ECO:0000244|PDB:1NRJ}.
FT   HELIX        96    105       {ECO:0000244|PDB:1NRJ}.
FT   HELIX       106    108       {ECO:0000244|PDB:1NRJ}.
FT   STRAND      109    117       {ECO:0000244|PDB:1NRJ}.
FT   HELIX       126    142       {ECO:0000244|PDB:1NRJ}.
FT   STRAND      149    154       {ECO:0000244|PDB:1NRJ}.
FT   HELIX       164    187       {ECO:0000244|PDB:1NRJ}.
FT   HELIX       214    216       {ECO:0000244|PDB:1NRJ}.
FT   STRAND      217    219       {ECO:0000244|PDB:1NRJ}.
FT   STRAND      221    225       {ECO:0000244|PDB:1NRJ}.
FT   TURN        228    231       {ECO:0000244|PDB:1NRJ}.
FT   HELIX       234    243       {ECO:0000244|PDB:1NRJ}.
SQ   SEQUENCE   244 AA;  26974 MW;  EA4AE9126B2A04B5 CRC64;
     MLSNTLIIAC LLVIGTTIAL IAVQKASSKT GIKQKSYQPS IIIAGPQNSG KTSLLTLLTT
     DSVRPTVVSQ EPLSAADYDG SGVTLVDFPG HVKLRYKLSD YLKTRAKFVK GLIFMVDSTV
     DPKKLTTTAE FLVDILSITE SSCENGIDIL IACNKSELFT ARPPSKIKDA LESEIQKVIE
     RRKKSLNEVE RKINEEDYAE NTLDVLQSTD GFKFANLEAS VVAFEGSINK RKISQWREWI
     DEKL
//

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