(data stored in SCRATCH zone)

SWISSPROT: RLMKL_ALCBS

ID   RLMKL_ALCBS             Reviewed;         704 AA.
AC   Q0VQU5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   11-DEC-2019, entry version 82.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase K/L {ECO:0000255|HAMAP-Rule:MF_01858};
DE   Includes:
DE     RecName: Full=23S rRNA m2G2445 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE              EC=2.1.1.173 {ECO:0000255|HAMAP-Rule:MF_01858};
DE     AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmL {ECO:0000255|HAMAP-Rule:MF_01858};
DE   Includes:
DE     RecName: Full=23S rRNA m7G2069 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE              EC=2.1.1.264 {ECO:0000255|HAMAP-Rule:MF_01858};
DE     AltName: Full=rRNA (guanine-N(7)-)-methyltransferase RlmK {ECO:0000255|HAMAP-Rule:MF_01858};
GN   Name=rlmL {ECO:0000255|HAMAP-Rule:MF_01858}; OrderedLocusNames=ABO_1005;
OS   Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 /
OS   SK2).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Alcanivoracaceae; Alcanivorax.
OX   NCBI_TaxID=393595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2;
RX   PubMed=16878126; DOI=10.1038/nbt1232;
RA   Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M.,
RA   Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C.,
RA   Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B.,
RA   McHardy A.C., Meyer F., Nechitaylo T., Puehler A., Regenhardt D., Rupp O.,
RA   Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J.,
RA   Weidner S., Kaiser O., Golyshin P.N.;
RT   "Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium
RT   Alcanivorax borkumensis.";
RL   Nat. Biotechnol. 24:997-1004(2006).
CC   -!- FUNCTION: Specifically methylates the guanine in position 2445
CC       (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.
CC       {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(2445) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(2445) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42740, Rhea:RHEA-COMP:10215, Rhea:RHEA-COMP:10216,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.173;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01858};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(2069) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(2069) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43772, Rhea:RHEA-COMP:10688, Rhea:RHEA-COMP:10689,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.264;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01858};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmKL family.
CC       {ECO:0000255|HAMAP-Rule:MF_01858}.
DR   EMBL; AM286690; CAL16453.1; -; Genomic_DNA.
DR   RefSeq; WP_011588289.1; NC_008260.1.
DR   SMR; Q0VQU5; -.
DR   STRING; 393595.ABO_1005; -.
DR   EnsemblBacteria; CAL16453; CAL16453; ABO_1005.
DR   KEGG; abo:ABO_1005; -.
DR   eggNOG; ENOG4105C4H; Bacteria.
DR   eggNOG; COG0116; LUCA.
DR   eggNOG; COG1092; LUCA.
DR   HOGENOM; HOG000218371; -.
DR   KO; K12297; -.
DR   OMA; GYEADPR; -.
DR   OrthoDB; 307177at2; -.
DR   BioCyc; ABOR393595:ABO_RS05205-MONOMER; -.
DR   Proteomes; UP000008871; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052915; F:23S rRNA (guanine(2445)-N(2))-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01858; 23SrRNA_methyltr_KL; 1.
DR   InterPro; IPR017244; 23SrRNA_methyltr_KL.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR019614; SAM-dep_methyl-trfase.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   Pfam; PF10672; Methyltrans_SAM; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   PIRSF; PIRSF037618; RNA_Mtase_bacteria_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 2.
DR   PROSITE; PS01261; UPF0020; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q0VQU5.
DR   SWISS-2DPAGE; Q0VQU5.
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..704
FT                   /note="Ribosomal RNA large subunit methyltransferase K/L"
FT                   /id="PRO_0000366724"
SQ   SEQUENCE   704 AA;  79404 MW;  E854646EB71B6ACC CRC64;
     MEFVATCMPG LADLLSDELG SLGISVTETG RAHVSFSGGV ADALKVCLWS RLSERVLLHL
     ATLGVTPDIA PEKLAASQDW LSLVGNNAPV HLHIEHGAEV RGDNRISAKR FIHALPEQFA
     ISREPKGSCC IRARLDLNEA HLWLDLAGEP LHRRGYRLAG GRAPLRETLA AAILWAVGWG
     KEGRHSALID PFCGSGTLVI EAALIAAGRA AGAQRQHYGF QQWRGCRRQL WLDAQKEAGE
     SGKRPVPAVS LKGFDSEAST LQLALQNAER AGVRNAIHFE RRELGALRRR DFVDNGIVVG
     NPPWGERLEE KPQAGWLHYA LGRILSVRAP GYQALLLGAD AEVMDRSGMN LEQQWRLKNG
     PFNNFIRLYS PRKQTPANVV KVADDSAFNV PEGAQPLLNR LRKNGKHLRR WLEREDIQCY
     RLYDRDLPEF NVAVDIYADQ VLVQEFKAPK TIDPEKAKER RDWAVTAVRA ALGVHREQVH
     LRTREKQKGN QQYQKLDAQG QYRVVREGQS QLLINLQDYL DTGLFLDHRP TRLRIAEEAS
     GKRFLNLFAY TGSATVHAAV GGAKRTVTVD SSKRYLDWAA CNLAANGFST DQHELARADT
     MRWLDECKEQ FDLVFCDPPT FSNNKSRSDF VVEEHHGDLI RKIMRCLEPG GVLYFSCNYR
     RFQMDESISK WYAVEDISRW SIPEDFRRNE KIHYCYAIRH VEDA
//

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