(data stored in SCRATCH zone)

SWISSPROT: Q13GM9_PARXL

ID   Q13GM9_PARXL            Unreviewed;       170 AA.
AC   Q13GM9;
DT   22-AUG-2006, integrated into UniProtKB/TrEMBL.
DT   22-AUG-2006, sequence version 1.
DT   07-JUN-2017, entry version 93.
DE   RecName: Full=Lipoprotein signal peptidase {ECO:0000256|HAMAP-Rule:MF_00161};
DE            EC=3.4.23.36 {ECO:0000256|HAMAP-Rule:MF_00161};
DE   AltName: Full=Prolipoprotein signal peptidase {ECO:0000256|HAMAP-Rule:MF_00161};
DE   AltName: Full=Signal peptidase II {ECO:0000256|HAMAP-Rule:MF_00161};
DE            Short=SPase II {ECO:0000256|HAMAP-Rule:MF_00161};
GN   Name=lspA {ECO:0000256|HAMAP-Rule:MF_00161};
GN   ORFNames=Bxe_A1105 {ECO:0000313|EMBL:ABE31841.1}, Bxe_A2904
GN   {ECO:0000313|EMBL:ABE30072.1}, Bxe_B1606
GN   {ECO:0000313|EMBL:ABE34358.1}, Bxe_B2910
GN   {ECO:0000313|EMBL:ABE33085.1}, Bxe_C0390
GN   {ECO:0000313|EMBL:ABE36297.1}, Bxe_C0884
GN   {ECO:0000313|EMBL:ABE36760.1};
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265 {ECO:0000313|EMBL:ABE36760.1, ECO:0000313|Proteomes:UP000001817};
RN   [1] {ECO:0000313|EMBL:ABE36760.1, ECO:0000313|Proteomes:UP000001817}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400 {ECO:0000313|EMBL:ABE36760.1,
RC   ECO:0000313|Proteomes:UP000001817};
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M.,
RA   Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A.,
RA   Marx C.J., Parnell J.J., Ramette A., Richardson P., Seeger M.,
RA   Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B.,
RA   Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp
RT   genome shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
RN   [2] {ECO:0000313|EMBL:ABE30072.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=LB400 {ECO:0000313|EMBL:ABE30072.1};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of Chromosome 1 of Burkholderia xenovorans LB400.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:ABE33085.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=LB400 {ECO:0000313|EMBL:ABE33085.1};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of Chromosome 2 of Burkholderia xenovorans LB400.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:ABE36760.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=LB400 {ECO:0000313|EMBL:ABE36760.1};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of Chromosome 3 of Burkholderia xenovorans LB400.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This protein specifically catalyzes the removal of
CC       signal peptides from prolipoproteins. {ECO:0000256|HAMAP-
CC       Rule:MF_00161, ECO:0000256|RuleBase:RU000594,
CC       ECO:0000256|SAAS:SAAS00395969}.
CC   -!- CATALYTIC ACTIVITY: Release of signal peptides from bacterial
CC       membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-
CC       (S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably
CC       Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small,
CC       neutral side chains. {ECO:0000256|HAMAP-Rule:MF_00161,
CC       ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00396003}.
CC   -!- PATHWAY: Protein modification; lipoprotein biosynthesis (signal
CC       peptide cleavage). {ECO:0000256|HAMAP-Rule:MF_00161,
CC       ECO:0000256|SAAS:SAAS00396035}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_00161}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_00161}.
CC   -!- SIMILARITY: Belongs to the peptidase A8 family.
CC       {ECO:0000256|HAMAP-Rule:MF_00161, ECO:0000256|RuleBase:RU004181,
CC       ECO:0000256|SAAS:SAAS00581686}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00161}.
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DR   EMBL; CP000270; ABE30072.1; -; Genomic_DNA.
DR   EMBL; CP000270; ABE31841.1; -; Genomic_DNA.
DR   EMBL; CP000271; ABE33085.1; -; Genomic_DNA.
DR   EMBL; CP000271; ABE34358.1; -; Genomic_DNA.
DR   EMBL; CP000272; ABE36297.1; -; Genomic_DNA.
DR   EMBL; CP000272; ABE36760.1; -; Genomic_DNA.
DR   RefSeq; WP_004863699.1; NZ_CP008762.1.
DR   SMR; Q13GM9; -.
DR   STRING; 266265.Bxe_C0884; -.
DR   MEROPS; A08.001; -.
DR   EnsemblBacteria; ABE30072; ABE30072; Bxe_A2904.
DR   EnsemblBacteria; ABE31841; ABE31841; Bxe_A1105.
DR   EnsemblBacteria; ABE33085; ABE33085; Bxe_B2910.
DR   EnsemblBacteria; ABE34358; ABE34358; Bxe_B1606.
DR   EnsemblBacteria; ABE36297; ABE36297; Bxe_C0390.
DR   EnsemblBacteria; ABE36760; ABE36760; Bxe_C0884.
DR   GeneID; 4010397; -.
DR   KEGG; bxb:DR64_3269; -.
DR   KEGG; bxb:DR64_5241; -.
DR   KEGG; bxb:DR64_586; -.
DR   KEGG; bxb:DR64_6909; -.
DR   KEGG; bxb:DR64_7565; -.
DR   KEGG; bxb:DR64_854; -.
DR   KEGG; bxe:Bxe_A1105; -.
DR   KEGG; bxe:Bxe_A2904; -.
DR   KEGG; bxe:Bxe_B1606; -.
DR   KEGG; bxe:Bxe_B2910; -.
DR   KEGG; bxe:Bxe_C0390; -.
DR   KEGG; bxe:Bxe_C0884; -.
DR   eggNOG; ENOG4107TEJ; Bacteria.
DR   eggNOG; COG0597; LUCA.
DR   HOGENOM; HOG000096993; -.
DR   KO; K03101; -.
DR   OMA; GFDFAIF; -.
DR   OrthoDB; POG091H059F; -.
DR   UniPathway; UPA00665; -.
DR   Proteomes; UP000001817; Chromosome 1.
DR   Proteomes; UP000001817; Chromosome 2.
DR   Proteomes; UP000001817; Chromosome 3.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00161; LspA; 1.
DR   InterPro; IPR001872; Peptidase_A8.
DR   PANTHER; PTHR33695:SF2; PTHR33695:SF2; 1.
DR   Pfam; PF01252; Peptidase_A8; 1.
DR   PRINTS; PR00781; LIPOSIGPTASE.
DR   TIGRFAMs; TIGR00077; lspA; 1.
DR   PROSITE; PS00855; SPASE_II; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q13GM9.
DR   SWISS-2DPAGE; Q13GM9.
KW   Aspartyl protease {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00112503};
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_00161};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|SAAS:SAAS00112556};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001817};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00112525,
KW   ECO:0000313|EMBL:ABE36760.1};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|SAAS:SAAS00112491};
KW   Protease {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00112585};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001817};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|SAAS:SAAS00112497};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_00161,
KW   ECO:0000256|SAAS:SAAS00112465}.
FT   SIGNAL        1     21       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        22    170       Lipoprotein signal peptidase.
FT                                {ECO:0000256|SAM:SignalP}.
FT                                /FTId=PRO_5009709945.
FT   TRANSMEM     68     85       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_00161}.
FT   TRANSMEM     97    116       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_00161}.
FT   TRANSMEM    136    156       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_00161}.
FT   ACT_SITE    113    113       {ECO:0000256|HAMAP-Rule:MF_00161}.
FT   ACT_SITE    140    140       {ECO:0000256|HAMAP-Rule:MF_00161}.
SQ   SEQUENCE   170 AA;  18815 MW;  ECE1C7415B3C10FB CRC64;
     MLIIGKKLSP YALLSISGLL AASDQAVKWL VQQSMAYGEY VSVTPFFNWV HLWNTGAAFS
     LFANGGGWQR YFFIGIAVVV SIFLIKLILE NRHKGEAIAY SLILGGAMGN LIDRVFRGYV
     VDSFDFYWRD WHWPAFNLAD IAIVLGALLF VSSSLLGKKA NTNAEPDGSD
//

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