(data stored in SCRATCH zone)

SWISSPROT: Q2IN98_ANADE

ID   Q2IN98_ANADE            Unreviewed;       248 AA.
AC   Q2IN98;
DT   07-MAR-2006, integrated into UniProtKB/TrEMBL.
DT   07-MAR-2006, sequence version 1.
DT   08-MAY-2019, entry version 98.
DE   RecName: Full=GTP cyclohydrolase-2 {ECO:0000256|HAMAP-Rule:MF_00179};
DE            EC=3.5.4.25 {ECO:0000256|HAMAP-Rule:MF_00179};
DE   AltName: Full=GTP cyclohydrolase II {ECO:0000256|HAMAP-Rule:MF_00179};
GN   Name=ribA {ECO:0000256|HAMAP-Rule:MF_00179};
GN   OrderedLocusNames=Adeh_0508 {ECO:0000313|EMBL:ABC80284.1};
OS   Anaeromyxobacter dehalogenans (strain 2CP-C).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter.
OX   NCBI_TaxID=290397 {ECO:0000313|EMBL:ABC80284.1, ECO:0000313|Proteomes:UP000001935};
RN   [1] {ECO:0000313|EMBL:ABC80284.1, ECO:0000313|Proteomes:UP000001935}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2CP-C {ECO:0000313|EMBL:ABC80284.1,
RC   ECO:0000313|Proteomes:UP000001935};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Proteomes:UP000001935}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2CP-C {ECO:0000313|Proteomes:UP000001935};
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of GTP to 2,5-diamino-6-
CC       ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and
CC       pyrophosphate. {ECO:0000256|HAMAP-Rule:MF_00179}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + 3 H2O = 2,5-diamino-6-hydroxy-4-(5-
CC         phosphoribosylamino)-pyrimidine + diphosphate + formate + 2
CC         H(+); Xref=Rhea:RHEA:23704, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:37565, ChEBI:CHEBI:58614; EC=3.5.4.25;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00179,
CC         ECO:0000256|SAAS:SAAS01116087};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00179};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00179};
CC   -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-
CC       6-(D-ribitylamino)uracil from GTP: step 1/4. {ECO:0000256|HAMAP-
CC       Rule:MF_00179}.
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase II family.
CC       {ECO:0000256|HAMAP-Rule:MF_00179}.
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DR   EMBL; CP000251; ABC80284.1; -; Genomic_DNA.
DR   RefSeq; WP_011419567.1; NC_007760.1.
DR   STRING; 290397.Adeh_0508; -.
DR   EnsemblBacteria; ABC80284; ABC80284; Adeh_0508.
DR   KEGG; ade:Adeh_0508; -.
DR   eggNOG; ENOG4107T1C; Bacteria.
DR   eggNOG; COG0807; LUCA.
DR   HOGENOM; HOG000115442; -.
DR   KO; K01497; -.
DR   OMA; AFGSRHC; -.
DR   BioCyc; ADEH290397:G1G5W-521-MONOMER; -.
DR   UniPathway; UPA00275; UER00400.
DR   Proteomes; UP000001935; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003935; F:GTP cyclohydrolase II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00641; GTP_cyclohydro2; 1.
DR   Gene3D; 3.40.50.10990; -; 1.
DR   HAMAP; MF_00179; RibA; 1.
DR   InterPro; IPR032677; GTP_cyclohydro_II.
DR   InterPro; IPR000926; RibA.
DR   InterPro; IPR036144; RibA-like_sf.
DR   Pfam; PF00925; GTP_cyclohydro2; 1.
DR   SUPFAM; SSF142695; SSF142695; 1.
DR   TIGRFAMs; TIGR00505; ribA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q2IN98.
DR   SWISS-2DPAGE; Q2IN98.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001935};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711691};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS01033620, ECO:0000313|EMBL:ABC80284.1};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711702};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711707};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001935};
KW   Riboflavin biosynthesis {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711711};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   DOMAIN       35    197       GTP_cyclohydro2. {ECO:0000259|Pfam:
FT                                PF00925}.
FT   NP_BIND      76     80       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   NP_BIND     119    121       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   ACT_SITE    153    153       Proton acceptor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   ACT_SITE    155    155       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        81     81       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        92     92       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        94     94       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   BINDING      97     97       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     141    141       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     176    176       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     181    181       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
SQ   SEQUENCE   248 AA;  27126 MW;  8CFEAB83E966F478 CRC64;
     MTGGGDALAQ LVLRDRDHEC EGFGPGRVCV RVVAVAGLPT RAGPFRIVAF WNNRDAKEHV
     ALVHGDVVGA SDVPVRLHSE CLTGDVMGSL RCDCRDQLTE GLAAIQREGR GVLLYLRQEG
     RGIGLINKIR AYALQEQGLD TVEANLALGF RDDERDYAVA AHMIHGLELR SIRLITNNPE
     KIRQLTAYRV EVSGRIPHVI PPGEHNRFYL ETKARRSGHL IDLDALAPRS EQADPVVVAP
     APQEPGAE
//

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