(data stored in SCRATCH zone)

SWISSPROT: Q3KJD3_PSEPF

ID   Q3KJD3_PSEPF            Unreviewed;       235 AA.
AC   Q3KJD3;
DT   08-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   08-NOV-2005, sequence version 1.
DT   08-MAY-2019, entry version 84.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase E {ECO:0000256|PIRNR:PIRNR015601};
DE            EC=2.1.1.193 {ECO:0000256|PIRNR:PIRNR015601};
GN   OrderedLocusNames=Pfl01_0379 {ECO:0000313|EMBL:ABA72123.1};
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922 {ECO:0000313|EMBL:ABA72123.1, ECO:0000313|Proteomes:UP000002704};
RN   [1] {ECO:0000313|EMBL:ABA72123.1, ECO:0000313|Proteomes:UP000002704}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1 {ECO:0000313|EMBL:ABA72123.1,
RC   ECO:0000313|Proteomes:UP000002704};
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Specifically methylates the N3 position of the uracil
CC       ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully
CC       assembled 30S ribosomal subunit. {ECO:0000256|PIRNR:PIRNR015601}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA =
CC         H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283,
CC         Rhea:RHEA-COMP:10284, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74502;
CC         EC=2.1.1.193; Evidence={ECO:0000256|PIRNR:PIRNR015601};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR015601}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC       {ECO:0000256|PIRNR:PIRNR015601}.
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DR   EMBL; CP000094; ABA72123.1; -; Genomic_DNA.
DR   RefSeq; WP_011332060.1; NC_007492.2.
DR   STRING; 205922.Pfl01_0379; -.
DR   EnsemblBacteria; ABA72123; ABA72123; Pfl01_0379.
DR   KEGG; pfo:Pfl01_0379; -.
DR   eggNOG; ENOG4108TSD; Bacteria.
DR   eggNOG; COG1385; LUCA.
DR   HOGENOM; HOG000015267; -.
DR   OMA; WQTPFLE; -.
DR   BioCyc; PFLU205922:G1G4S-383-MONOMER; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR30027; PTHR30027; 1.
DR   Pfam; PF04452; Methyltrans_RNA; 1.
DR   PIRSF; PIRSF015601; MTase_slr0722; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00046; TIGR00046; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q3KJD3.
DR   SWISS-2DPAGE; Q3KJD3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002704};
KW   Cytoplasm {ECO:0000256|PIRNR:PIRNR015601};
KW   Methyltransferase {ECO:0000256|PIRNR:PIRNR015601};
KW   rRNA processing {ECO:0000256|PIRNR:PIRNR015601};
KW   S-adenosyl-L-methionine {ECO:0000256|PIRNR:PIRNR015601};
KW   Transferase {ECO:0000256|PIRNR:PIRNR015601}.
SQ   SEQUENCE   235 AA;  26058 MW;  3FD9C5C1C26AFF32 CRC64;
     MNLLLLEEAD FIAADRVVLR DRRLTHMQEV HRSEVGDSLR VGCIGGLMGS AELLRLDAGE
     AELRVNLDQP PPAKLPLTLV LALPRPKMLR RVFQTVATMG VSKVILVNSY RVEKSFWQTP
     FLEPDAIREQ LILGLEQARD TVLPEIIIEK RFKPFVEDRL PAITDGTLGL VGHPGNFPPC
     PRALNEPVTL AIGPEGGWIP YEIDLLAKAG LQPVQLGERI LRVETAVTAL LARLF
//

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