(data stored in SCRATCH zone)

SWISSPROT: Q3KJW2_PSEPF

ID   Q3KJW2_PSEPF            Unreviewed;       136 AA.
AC   Q3KJW2;
DT   08-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   08-NOV-2005, sequence version 1.
DT   08-MAY-2019, entry version 98.
DE   RecName: Full=5-hydroxyisourate hydrolase {ECO:0000256|RuleBase:RU361270};
DE            Short=HIU hydrolase {ECO:0000256|RuleBase:RU361270};
DE            Short=HIUHase {ECO:0000256|RuleBase:RU361270};
DE            EC=3.5.2.17 {ECO:0000256|RuleBase:RU361270};
GN   OrderedLocusNames=Pfl01_0200 {ECO:0000313|EMBL:ABA71944.1};
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922 {ECO:0000313|EMBL:ABA71944.1, ECO:0000313|Proteomes:UP000002704};
RN   [1] {ECO:0000313|EMBL:ABA71944.1, ECO:0000313|Proteomes:UP000002704}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1 {ECO:0000313|EMBL:ABA71944.1,
RC   ECO:0000313|Proteomes:UP000002704};
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-
CC         dihydro-1H-imidazole-5-carboxylate + H(+); Xref=Rhea:RHEA:23736,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18072,
CC         ChEBI:CHEBI:58639; EC=3.5.2.17;
CC         Evidence={ECO:0000256|RuleBase:RU361270};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|RuleBase:RU361270}.
CC   -!- SIMILARITY: Belongs to the transthyretin family. 5-hydroxyisourate
CC       hydrolase subfamily. {ECO:0000256|RuleBase:RU361270}.
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DR   EMBL; CP000094; ABA71944.1; -; Genomic_DNA.
DR   RefSeq; WP_011331909.1; NC_007492.2.
DR   STRING; 205922.Pfl01_0200; -.
DR   EnsemblBacteria; ABA71944; ABA71944; Pfl01_0200.
DR   KEGG; pfo:Pfl01_0200; -.
DR   eggNOG; ENOG4105VT2; Bacteria.
DR   eggNOG; COG2351; LUCA.
DR   HOGENOM; HOG000251776; -.
DR   KO; K07127; -.
DR   OMA; DEHYHVP; -.
DR   BioCyc; PFLU205922:G1G4S-203-MONOMER; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0033971; F:hydroxyisourate hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.180; -; 1.
DR   InterPro; IPR014306; Hydroxyisourate_hydrolase.
DR   InterPro; IPR000895; Transthyretin/HIU_hydrolase.
DR   InterPro; IPR023416; Transthyretin/HIU_hydrolase_d.
DR   InterPro; IPR036817; Transthyretin/HIU_hydrolase_sf.
DR   InterPro; IPR023419; Transthyretin_CS.
DR   PANTHER; PTHR10395; PTHR10395; 1.
DR   Pfam; PF00576; Transthyretin; 1.
DR   PRINTS; PR00189; TRNSTHYRETIN.
DR   SMART; SM00095; TR_THY; 1.
DR   SUPFAM; SSF49472; SSF49472; 1.
DR   TIGRFAMs; TIGR02962; hdxy_isourate; 1.
DR   PROSITE; PS00769; TRANSTHYRETIN_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q3KJW2.
DR   SWISS-2DPAGE; Q3KJW2.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002704};
KW   Hydrolase {ECO:0000256|RuleBase:RU361270};
KW   Purine metabolism {ECO:0000256|RuleBase:RU361270};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL        1     22       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        23    136       5-hydroxyisourate hydrolase.
FT                                {ECO:0000256|SAM:SignalP}.
FT                                /FTId=PRO_5004227603.
FT   DOMAIN       22    135       TR_THY. {ECO:0000259|SMART:SM00095}.
SQ   SEQUENCE   136 AA;  15116 MW;  BDCB38EC03894DDE CRC64;
     MTPLRITFAA LGLSAFSSLA LAAGNPLSVH VLNLENGLPS PGINVTLERH VGQNWQPLAQ
     GTTNEQGRIA ELFPANKPFE AGEYRVVFKT GEYFEKTKHE TFFPEIPVIF KVQQTDQHYH
     IPLLLSPYGF STYRGS
//

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