(data stored in SCRATCH zone)

SWISSPROT: Q3KK31_PSEPF

ID   Q3KK31_PSEPF            Unreviewed;       648 AA.
AC   Q3KK31;
DT   08-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   08-NOV-2005, sequence version 1.
DT   08-MAY-2019, entry version 99.
DE   SubName: Full=Diguanylate cyclase/phosphodiesterase {ECO:0000313|EMBL:ABA71875.1};
DE   Flags: Precursor;
GN   Name=lapD {ECO:0000313|EMBL:ABA71875.1};
GN   OrderedLocusNames=Pfl01_0131 {ECO:0000313|EMBL:ABA71875.1};
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922 {ECO:0000313|EMBL:ABA71875.1, ECO:0000313|Proteomes:UP000002704};
RN   [1] {ECO:0000313|EMBL:ABA71875.1, ECO:0000313|Proteomes:UP000002704}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1 {ECO:0000313|EMBL:ABA71875.1,
RC   ECO:0000313|Proteomes:UP000002704};
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
RN   [2] {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:3PJU, ECO:0000213|PDB:3PJV}
RP   X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 22-151 IN COMPLEX WITH
RP   CYCLIC DIGUANOSINE MONOPHOSPHATE.
RX   PubMed=21304926; DOI=10.1371/journal.pbio.1000588;
RA   Navarro M.V., Newell P.D., Krasteva P.V., Chatterjee D., Madden D.R.,
RA   O'Toole G.A., Sondermann H.;
RT   "Structural basis for c-di-GMP-mediated inside-out signaling
RT   controlling periplasmic proteolysis.";
RL   PLoS Biol. 9:e1000588-e1000588(2011).
CC   -!- INTERACTION:
CC       Self; NbExp=5; IntAct=EBI-6418896, EBI-6418896;
CC       Q3KK32:lapF; NbExp=6; IntAct=EBI-6418896, EBI-6418902;
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DR   EMBL; CP000094; ABA71875.1; -; Genomic_DNA.
DR   RefSeq; WP_011331847.1; NC_007492.2.
DR   PDB; 3PJT; X-ray; 2.52 A; A/B=400-648.
DR   PDB; 3PJU; X-ray; 2.50 A; A=400-648.
DR   PDB; 3PJV; X-ray; 1.77 A; D/F=22-151.
DR   PDB; 3PJW; X-ray; 3.10 A; A=220-648.
DR   PDB; 3PJX; X-ray; 2.00 A; A=220-648.
DR   PDBsum; 3PJT; -.
DR   PDBsum; 3PJU; -.
DR   PDBsum; 3PJV; -.
DR   PDBsum; 3PJW; -.
DR   PDBsum; 3PJX; -.
DR   SMR; Q3KK31; -.
DR   DIP; DIP-58963N; -.
DR   IntAct; Q3KK31; 2.
DR   STRING; 205922.Pfl01_0131; -.
DR   EnsemblBacteria; ABA71875; ABA71875; Pfl01_0131.
DR   KEGG; pfo:Pfl01_0131; -.
DR   eggNOG; ENOG41062P1; Bacteria.
DR   eggNOG; ENOG410XSQH; LUCA.
DR   HOGENOM; HOG000219743; -.
DR   OMA; FGGRFSM; -.
DR   BioCyc; PFLU205922:G1G4S-136-MONOMER; -.
DR   EvolutionaryTrace; Q3KK31; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   CDD; cd01948; EAL; 1.
DR   CDD; cd01949; GGDEF; 1.
DR   CDD; cd06225; HAMP; 1.
DR   Gene3D; 3.20.20.450; -; 1.
DR   InterPro; IPR001633; EAL_dom.
DR   InterPro; IPR035919; EAL_sf.
DR   InterPro; IPR000160; GGDEF_dom.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR032244; LapD_MoxY_N.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF00563; EAL; 1.
DR   Pfam; PF00990; GGDEF; 1.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF16448; LapD_MoxY_N; 1.
DR   SMART; SM00052; EAL; 1.
DR   SMART; SM00267; GGDEF; 1.
DR   SMART; SM00304; HAMP; 1.
DR   SUPFAM; SSF141868; SSF141868; 1.
DR   SUPFAM; SSF55073; SSF55073; 1.
DR   TIGRFAMs; TIGR00254; GGDEF; 1.
DR   PROSITE; PS50883; EAL; 1.
DR   PROSITE; PS50887; GGDEF; 1.
DR   PROSITE; PS50885; HAMP; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q3KK31.
DR   SWISS-2DPAGE; Q3KK31.
KW   3D-structure {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:3PJU,
KW   ECO:0000213|PDB:3PJV, ECO:0000213|PDB:3PJW};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002704};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Nucleotide-binding {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:3PJU};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    152    170       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN      171    223       HAMP. {ECO:0000259|PROSITE:PS50885}.
FT   DOMAIN      265    400       GGDEF. {ECO:0000259|PROSITE:PS50887}.
FT   DOMAIN      410    648       EAL. {ECO:0000259|PROSITE:PS50883}.
FT   NP_BIND     446    450       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
FT   NP_BIND     560    561       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
FT   NP_BIND     618    620       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
FT   COILED      215    235       {ECO:0000256|SAM:Coils}.
FT   BINDING     431    431       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJU}.
FT   BINDING     462    462       Cyclic diguanosine monophosphate; via
FT                                amide nitrogen. {ECO:0000213|PDB:3PJT,
FT                                ECO:0000213|PDB:3PJU}.
FT   BINDING     478    478       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
FT   BINDING     499    499       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
FT   BINDING     639    639       Cyclic diguanosine monophosphate.
FT                                {ECO:0000213|PDB:3PJT, ECO:0000213|PDB:
FT                                3PJU}.
SQ   SEQUENCE   648 AA;  71786 MW;  0EC47D6D9BF3C233 CRC64;
     MSLFKQLLIA ICLFLVVAFT GSFMVSLESS RTQYVNQLRS HAQDAATALA LSLTPNIDDP
     AMVELLVSSI FDSGYYSSIR VVDLKTDQTI VERNGIPAVT NVPDWFVKLI GLEPAGGDAL
     VSRGWEQAAR VEVVSHPMFA LAKLWQSALG SLGWLLVCGA VSAVLGALLL RRQLKPLDYM
     VKQSHAIARR EFLSLPDLPR TPELRRVVLA MNQMVEKLKA LFQEQAERSE KLRTESYQDN
     LTGLANRRYF EMQLNARVSN PEQASSGYLL LLRVKDLAGL NQRLGGQRTD ELLKAVGEQL
     SRECAKYPET QNLVTRIRGG EFAVLAPGMT REEALQLAQS LDSALSSLYA TGATDVAAVA
     SIGLAPFAHG DSPQAVLSLG DQALAQAEGQ GEQNWACLDQ SLVADVGDDH HAWHRLLDQA
     LNQRRFELFF QPVVAAQDTQ LVLHYKVLSR LLDEQGQTIP AGRFLPWLER FGWTARLDRL
     MLERVLEQMA GHEESLALNL SSATLADPQA LNKVFEILRA HSNLGARLTL EIGEEQLPEQ
     AVLEQLTRRL RELGFSLSLQ RFGGRFSMIG NLARLGLAYL KIDGSYIRAI DQESDKRLFI
     EAIQRAAHSI DLPLIAERVE TEGELSVIRE MGLYGVQGQL FGEPKPWG
//

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