(data stored in ACNUC21562 zone)

SWISSPROT: MRGB2_MOUSE

ID   MRGB2_MOUSE             Reviewed;         338 AA.
AC   Q3KNA1; Q8CDY4; Q91ZC2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   08-MAY-2019, entry version 97.
DE   RecName: Full=Mas-related G-protein coupled receptor member B2;
GN   Name=Mrgprb2; Synonyms=Mrgb2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=11551509; DOI=10.1016/S0092-8674(01)00483-4;
RA   Dong X., Han S.-K., Zylka M.J., Simon M.I., Anderson D.J.;
RT   "A diverse family of GPCRs expressed in specific subsets of
RT   nociceptive sensory neurons.";
RL   Cell 106:619-632(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Head;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=25517090; DOI=10.1038/nature14022;
RA   McNeil B.D., Pundir P., Meeker S., Han L., Undem B.J., Kulka M.,
RA   Dong X.;
RT   "Identification of a mast-cell-specific receptor crucial for pseudo-
RT   allergic drug reactions.";
RL   Nature 519:237-241(2015).
CC   -!- FUNCTION: Mast cell-specific receptor for basic secretagogues,
CC       i.e. cationic amphiphilic drugs, as well as endo- or exogenous
CC       peptides, consisting of a basic head group and a hydrophobic core.
CC       Recognizes and binds small molecules containing a cyclized
CC       tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular
CC       blocking drugs (NMBDs), including tubocurarine and atracurium. In
CC       response to these compounds, mediates pseudo-allergic reactions
CC       characterized by histamine release, inflammation and airway
CC       contraction (PubMed:25517090). {ECO:0000269|PubMed:25517090}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Mast cell-specific.
CC       {ECO:0000269|PubMed:25517090}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype in normal conditions.
CC       Absence of pseudo-allergic reactions in response to small-molecule
CC       therapeutic drugs: secretagogue-induced histamine release,
CC       inflammation and airway contraction are abolished.
CC       {ECO:0000269|PubMed:25517090}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       Mas subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   -!- CAUTION: In spite of its official gene name, this protein may be
CC       the functional ortholog of human MRGPRX2, in terms of expression
CC       pattern and pharmacology. {ECO:0000269|PubMed:25517090}.
DR   EMBL; AY042200; AAK91796.1; -; Genomic_DNA.
DR   EMBL; AK029369; BAC26422.1; -; mRNA.
DR   EMBL; BC107390; AAI07391.1; -; mRNA.
DR   EMBL; BC107391; AAI07392.1; -; mRNA.
DR   CCDS; CCDS21303.1; -.
DR   RefSeq; NP_780740.2; NM_175531.4.
DR   STRING; 10090.ENSMUSP00000061878; -.
DR   iPTMnet; Q3KNA1; -.
DR   PhosphoSitePlus; Q3KNA1; -.
DR   PaxDb; Q3KNA1; -.
DR   PRIDE; Q3KNA1; -.
DR   Ensembl; ENSMUST00000052730; ENSMUSP00000061878; ENSMUSG00000050425.
DR   GeneID; 243979; -.
DR   KEGG; mmu:243979; -.
DR   UCSC; uc009hat.2; mouse.
DR   CTD; 243979; -.
DR   MGI; MGI:2441674; Mrgprb2.
DR   eggNOG; ENOG410J9NM; Eukaryota.
DR   eggNOG; ENOG4111AUB; LUCA.
DR   GeneTree; ENSGT00950000182854; -.
DR   HOGENOM; HOG000015412; -.
DR   InParanoid; Q3KNA1; -.
DR   KO; K08396; -.
DR   OMA; NAIVLWF; -.
DR   OrthoDB; 1123658at2759; -.
DR   PhylomeDB; Q3KNA1; -.
DR   TreeFam; TF336336; -.
DR   PRO; PR:Q3KNA1; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   Bgee; ENSMUSG00000050425; Expressed in 8 organ(s), highest expression level in zone of skin.
DR   ExpressionAtlas; Q3KNA1; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; ISO:MGI.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; ISO:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:1902349; P:response to chloroquine; ISO:MGI.
DR   GO; GO:0007165; P:signal transduction; ISO:MGI.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR026234; MRGPCRFAMILY.
DR   PANTHER; PTHR11334; PTHR11334; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR02108; MRGPCRFAMILY.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q3KNA1.
DR   SWISS-2DPAGE; Q3KNA1.
KW   Cell membrane; Complete proteome; G-protein coupled receptor;
KW   Glycoprotein; Membrane; Receptor; Reference proteome; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN         1    338       Mas-related G-protein coupled receptor
FT                                member B2.
FT                                /FTId=PRO_0000304878.
FT   TOPO_DOM      1     40       Extracellular. {ECO:0000255}.
FT   TRANSMEM     41     61       Helical; Name=1. {ECO:0000255}.
FT   TOPO_DOM     62     89       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     90    110       Helical; Name=2. {ECO:0000255}.
FT   TOPO_DOM    111    111       Extracellular. {ECO:0000255}.
FT   TRANSMEM    112    132       Helical; Name=3. {ECO:0000255}.
FT   TOPO_DOM    133    157       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    158    178       Helical; Name=4. {ECO:0000255}.
FT   TOPO_DOM    179    191       Extracellular. {ECO:0000255}.
FT   TRANSMEM    192    212       Helical; Name=5. {ECO:0000255}.
FT   TOPO_DOM    213    231       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    232    252       Helical; Name=6. {ECO:0000255}.
FT   TOPO_DOM    253    268       Extracellular. {ECO:0000255}.
FT   TRANSMEM    269    289       Helical; Name=7. {ECO:0000255}.
FT   TOPO_DOM    290    338       Cytoplasmic. {ECO:0000255}.
FT   CARBOHYD      9      9       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     18     18       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     23     23       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CONFLICT     28     28       I -> F (in Ref. 2; BAC26422).
FT                                {ECO:0000305}.
FT   CONFLICT    263    263       E -> K (in Ref. 3; AAI07391/AAI07392).
FT                                {ECO:0000305}.
SQ   SEQUENCE   338 AA;  38832 MW;  1B0A091D67C868B9 CRC64;
     MSGDFLIKNL STSAWKTNIT VLNGSYYIDT SVCVTRNQAM ILLSIIISLV GMGLNAIVLW
     FLGIRMHTNA FTVYILNLAM ADFLYLCSQF VICLLIAFYI FYSIDINIPL VLYVVPIFAY
     LSGLSILSTI SIERCLSVIW PIWYRCKRPR HTSAITCFVL WVMSLLLGLL EGKACGLLFN
     SFDSYWCETF DVITNIWSVV FFGVLCGSSL TLLVRIFCGS QRIPMTRLYV TITLTVLVFL
     IFGLPFGIYW ILYQWISNFY YVEICNFYLE ILFLSCVNSC MNPIIYFLVG SIRHRRFRRK
     TLKLLLQRAM QDTPEEEQSG NKSSSEHPEE LETVQSCS
//

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