(data stored in SCRATCH zone)

SWISSPROT: Q4ZZF4_PSEU2

ID   Q4ZZF4_PSEU2            Unreviewed;       739 AA.
AC   Q4ZZF4;
DT   07-JUN-2005, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2005, sequence version 1.
DT   08-MAY-2019, entry version 97.
DE   RecName: Full=Primosomal protein N' {ECO:0000256|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000256|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000256|HAMAP-Rule:MF_00983};
GN   OrderedLocusNames=Psyr_0398 {ECO:0000313|EMBL:AAY35468.1};
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918 {ECO:0000313|EMBL:AAY35468.1, ECO:0000313|Proteomes:UP000000426};
RN   [1] {ECO:0000313|EMBL:AAY35468.1, ECO:0000313|Proteomes:UP000000426}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a {ECO:0000313|EMBL:AAY35468.1,
RC   ECO:0000313|Proteomes:UP000000426};
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae
RT   pv. syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000256|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000256|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_00983}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000075; AAY35468.1; -; Genomic_DNA.
DR   RefSeq; WP_011266372.1; NC_007005.1.
DR   RefSeq; YP_233506.1; NC_007005.1.
DR   STRING; 205918.Psyr_0398; -.
DR   EnsemblBacteria; AAY35468; AAY35468; Psyr_0398.
DR   GeneID; 3365874; -.
DR   KEGG; psb:Psyr_0398; -.
DR   PATRIC; fig|205918.7.peg.412; -.
DR   eggNOG; ENOG4105C25; Bacteria.
DR   eggNOG; COG1198; LUCA.
DR   HOGENOM; HOG000037413; -.
DR   KO; K04066; -.
DR   OMA; FQNRRGY; -.
DR   BioCyc; PSYR205918:G1G4J-399-MONOMER; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.8.630; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q4ZZF4.
DR   SWISS-2DPAGE; Q4ZZF4.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000426};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00983};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Helicase {ECO:0000256|HAMAP-Rule:MF_00983,
KW   ECO:0000313|EMBL:AAY35468.1};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00983};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00983}.
FT   DOMAIN      215    381       Helicase ATP-binding.
FT                                {ECO:0000259|PROSITE:PS51192}.
FT   DOMAIN      457    632       Helicase C-terminal.
FT                                {ECO:0000259|PROSITE:PS51194}.
FT   ZN_FING     440    452       C4-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00983}.
FT   ZN_FING     467    483       C4-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00983}.
SQ   SEQUENCE   739 AA;  81972 MW;  65453589B5EDD5E5 CRC64;
     MPDAILRLAL PSPLRRLFDY RAPAGVSRSA LQPGMRLRVP FGRREMIGIL VEVVDHSEVP
     ADKLKPAIAL LDSEAPLPPA LFKLCLWTSQ YYQHSLGDTL SWALPVLLRQ GELAESRQER
     FWHVAPGASV DDPRIARAPK QREALTTLAQ HPHGVAHQLL SKLMLNRDSL NLLLAKELVY
     VEVRSHAPSA RHEHWLAQPE LPLNTEQRAA YEAIRAGFDS FHAFLLAGVT GSGKTEVYLQ
     LIRETLEAGK QALVLIPEIN LGPQTLARFE QRFNARIALI HSAVNDRERL DAWLAARDGE
     ADIIIGTRSA LFTPMKNPGL IIIDEEHDGS YKQQEGLRYH ARDLALVRAR QENIPIVLGS
     ATPSLESLHN AYTGRYGLLR LNQRAGGAQQ PRFMRLDVKS RPLDSGISGP MQQAIGQTLA
     AGQQVLVFLN RRGFAPTLLC HDCGWMSGCQ RCDARMTVHQ RSGELRCHHC GYVERVPRQC
     PSCGKVDLRP VGAGTERAEE RLAILFPDYP VLRVDRDSTS RKDAMNQLFT TIQRGQPCIL
     VGTQMLAKGH HFPRVTLVSI LDADGGLFSG DFRASERMAQ LIVQVAGRAG RAEEPGKVII
     QTHLADHPLL IQLTEQGYFA FAEQALSERR SASLPPFSHL ALLRAEAHKP GQAETFLDQA
     CSDAEQLVAQ MALGGIELLG PVPAPMERRA GRYRAQLLVQ SSARAPLHKL LATWLLALEQ
     MPSGRQVRWS LDVDPVDLY
//

If you have problems or comments...

PBIL Back to PBIL home page