(data stored in SCRATCH zone)

SWISSPROT: Q4ZZS9_PSEU2

ID   Q4ZZS9_PSEU2            Unreviewed;       921 AA.
AC   Q4ZZS9;
DT   07-JUN-2005, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2005, sequence version 1.
DT   08-MAY-2019, entry version 111.
DE   RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN   Name=polA {ECO:0000256|RuleBase:RU004460};
GN   OrderedLocusNames=Psyr_0270 {ECO:0000313|EMBL:AAY35343.1};
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918 {ECO:0000313|EMBL:AAY35343.1, ECO:0000313|Proteomes:UP000000426};
RN   [1] {ECO:0000313|EMBL:AAY35343.1, ECO:0000313|Proteomes:UP000000426}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a {ECO:0000313|EMBL:AAY35343.1,
RC   ECO:0000313|Proteomes:UP000000426};
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae
RT   pv. syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 5'-3' exonuclease activity.
CC       {ECO:0000256|RuleBase:RU004460}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU004460};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC       {ECO:0000256|RuleBase:RU004460}.
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DR   EMBL; CP000075; AAY35343.1; -; Genomic_DNA.
DR   RefSeq; WP_011266289.1; NC_007005.1.
DR   RefSeq; YP_233381.1; NC_007005.1.
DR   STRING; 205918.Psyr_0270; -.
DR   EnsemblBacteria; AAY35343; AAY35343; Psyr_0270.
DR   GeneID; 3365746; -.
DR   KEGG; psb:Psyr_0270; -.
DR   PATRIC; fig|205918.7.peg.270; -.
DR   eggNOG; ENOG4105C2M; Bacteria.
DR   eggNOG; COG0258; LUCA.
DR   eggNOG; COG0749; LUCA.
DR   HOGENOM; HOG000020999; -.
DR   KO; K02335; -.
DR   OMA; ETGRVHT; -.
DR   BioCyc; PSYR205918:G1G4J-271-MONOMER; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR002421; 5-3_exonuclease_N.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q4ZZS9.
DR   SWISS-2DPAGE; Q4ZZS9.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000426};
KW   DNA damage {ECO:0000256|RuleBase:RU004460};
KW   DNA repair {ECO:0000256|RuleBase:RU004460};
KW   DNA replication {ECO:0000256|RuleBase:RU004460};
KW   DNA-binding {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000256|SAAS:SAAS00882656};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU004460};
KW   Hydrolase {ECO:0000256|SAAS:SAAS00427537};
KW   Nuclease {ECO:0000256|SAAS:SAAS00427491};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:AAY35343.1};
KW   Transferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:AAY35343.1}.
FT   DOMAIN        4    266       53EXOc. {ECO:0000259|SMART:SM00475}.
FT   DOMAIN      314    509       3'-5' exonuclease. {ECO:0000259|SMART:
FT                                SM00474}.
FT   DOMAIN      679    885       POLAc. {ECO:0000259|SMART:SM00482}.
SQ   SEQUENCE   921 AA;  100960 MW;  C379A909EC88467B CRC64;
     MTQAPLVLVD GSSYLYRAFH ALPPLATSKG LPTGAVKGVL NMLKSLRRQY PDSPLAVVFD
     AKGGTFRDAL YNDYKANRPS MPDDLRVQVD LLHACVKGMG YPFLCVEGVE ADDVIGTLAR
     SSAAADRPVV ISTGDKDMAQ LVDGHITLVN TMTGSVLDVA GVKEKFGVGP EHIIDYLALM
     GDKVDNIPGV PGVGEKTAVG LLVGIGGGIK ELYENLDKVA SLPIRGAKTL AAKLEEHREM
     AFLSYELATI KIDVPLDIEL DQLHCGEPDR DTLMELYAEL EFKSWIEDLQ RDAKRAGQEL
     TIEEPTAEAR EAAYEVILEQ GQFDAWLKKL QAAPLFAFVT QSNGTDAQRA QLVGLSFAIQ
     THEAAYIPLT HSYMGVPQQL DRDTVLKALK PLLEDPDKIK VGQHAKFAIN LLANCAIDGD
     QAQGIDLQGV RFDTILESYV LDSTATRHDR DSLVAKYLTH TPINFQEIAG KGAKQLSFDQ
     IALEQAGNYA AEEADLTLRL HEVFDARLAA IPTLQPVLND IEMPLVPVLA RIERQGALVD
     ANLLGIQSVE LGDKMTALER EAFAIAGEEF NLGSPKQLGV ILYEKLGMPI LSKTATGQAS
     TAEAVLAELA EQDFPLPKVL MQYRSMSKLK STYTDRLPEQ INPRTGRIHT SYHQAVAVTG
     RLSSSDPNLQ NIPIRTAEGR RIRQAFVAPK GYKLLAADYS QIELRIMAHL AQDEGLLHAF
     RNDLDVHRAT AAEVFGVELE NVTTDMRRSA KAINFGLIYG MSAFGLAKQI GVDRKQSQAY
     VDRYFARYPG VLNYMERTRA QAAEQGFVET IFGRRLYLPD INAKNQSLRK GAERMAINAP
     MQGTAADIIK KAMVAVNGWL DESGLDARVI LQVHDELVLE VREDLVDQIS EQIRPHMSGA
     AELAVPLLVE VGVGNNWDEA H
//

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