(data stored in SCRATCH zone)

SWISSPROT: Q750C0_ASHGO

ID   Q750C0_ASHGO            Unreviewed;       589 AA.
AC   Q750C0;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   11-DEC-2019, entry version 125.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000256|RuleBase:RU003748};
DE            EC=6.1.1.6 {ECO:0000256|RuleBase:RU003748};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000256|RuleBase:RU003748};
GN   ORFNames=AGOS_AGR037C {ECO:0000313|EMBL:AAS54526.1};
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811 {ECO:0000313|EMBL:AAS54526.1, ECO:0000313|Proteomes:UP000000591};
RN   [1] {ECO:0000313|EMBL:AAS54526.1, ECO:0000313|Proteomes:UP000000591}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056
RC   {ECO:0000313|Proteomes:UP000000591};
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Pohlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2] {ECO:0000313|Proteomes:UP000000591}
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056
RC   {ECO:0000313|Proteomes:UP000000591};
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000256|RuleBase:RU003748};
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DR   EMBL; AE016820; AAS54526.1; -; Genomic_DNA.
DR   RefSeq; NP_986702.1; NM_211764.1.
DR   STRING; 33169.AAS54526; -.
DR   EnsemblFungi; AAS54526; AAS54526; AGOS_AGR037C.
DR   GeneID; 4623002; -.
DR   KEGG; ago:AGOS_AGR037C; -.
DR   HOGENOM; HOG000236577; -.
DR   InParanoid; Q750C0; -.
DR   KO; K04567; -.
DR   OMA; EIFGEKC; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00775; LysRS_core; 1.
DR   HAMAP; MF_00252; Lys_tRNA_synth_class2; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR002313; Lys-tRNA-ligase_II.
DR   InterPro; IPR034762; Lys-tRNA-ligase_II_bac/euk.
DR   InterPro; IPR018149; Lys-tRNA-synth_II_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PIRSF; PIRSF039101; LysRS2; 1.
DR   PRINTS; PR00982; TRNASYNTHLYS.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00499; lysS_bact; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q750C0.
DR   SWISS-2DPAGE; Q750C0.
KW   Reference proteome {ECO:0000313|Proteomes:UP000000591}.
FT   DOMAIN          250..578
FT                   /note="AA_TRNA_LIGASE_II"
FT                   /evidence="ECO:0000259|PROSITE:PS50862"
FT   REGION          25..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   589 AA;  67782 MW;  8089F20583145414 CRC64;
     MSEEAAVQQV AENVANLHLD EVTGEMVSKS ELKKRIKQRQ VEAKKAAKKA TSKPKPEPKK
     KVENMADLDP SKYFEARSRQ IQELRKTQEP NPYPHKFNVT IGLPAFLNKY AHLQRGETLP
     EERVSIAGRI HAKRESGSKL RFYVLHADGV EVQVMSQLQD YHSEEAYEQD HGLLKRGDIV
     GVEGYVGRTQ PKKGGEGEIS VFVSRIQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN
     KDARGRFITR SKIITYIRKF LDNRDFIEVE TPMMNVIAGG ATAKPFVTHH NDLDMQMYMR
     IAPELFLKQL VVGGMDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV YDLMDMTELL
     FSEMVKEITG DYKIKFHPDP NDPSKEYDIN FARPWKRINM IEELEKRFNV TFPAGDQLHT
     AETGEFLKKI LSDNKMDCPP PLTNARMLDK LVGELEDTCI DPTFIFGHPQ MMSPLAKHSR
     DQPGLCERFE VFVATKEICN AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LIDETFCNAL
     EYGLPPTGGW GCGVDRLAMF LTDSNTIREV LLFPTLKPDV LKEDLKKEN
//

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