(data stored in ACNUC3292 zone)

SWISSPROT: SPAG1_MOUSE

ID   SPAG1_MOUSE             Reviewed;         901 AA.
AC   Q80ZX8; Q8CCK7; Q9QZJ3; Q9QZJ4;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   11-DEC-2019, entry version 139.
DE   RecName: Full=Sperm-associated antigen 1;
DE   AltName: Full=Infertility-related sperm protein Spag-1;
DE   AltName: Full=TPR-containing protein involved in spermatogenesis;
DE            Short=TPIS;
GN   Name=Spag1; Synonyms=Tpis;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), DEVELOPMENTAL STAGE, AND
RP   TISSUE SPECIFICITY.
RC   STRAIN=ICR; TISSUE=Fetal skin, and Testis;
RX   PubMed=10527845; DOI=10.1006/bbrc.1999.1477;
RA   Takaishi M., Huh N.-H.;
RT   "A tetratricopeptide repeat-containing protein gene, tpis, whose expression
RT   is induced with differentiation of spermatogenic cells.";
RL   Biochem. Biophys. Res. Commun. 264:81-85(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   STRAIN=C57BL/6J; TISSUE=Olfactory bulb;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Olfactory epithelium;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351; SER-359; SER-739 AND
RP   SER-740, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Kidney, Lung, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: May play a role in the cytoplasmic assembly of the ciliary
CC       dynein arms (By similarity). May play a role in fertilization. Binds
CC       GTP and has GTPase activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q80ZX8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q80ZX8-2; Sequence=VSP_012684;
CC       Name=3;
CC         IsoId=Q80ZX8-3; Sequence=VSP_012685;
CC   -!- TISSUE SPECIFICITY: Detected in cerebellum, tongue, esophagus,
CC       forestomach, sperm and testis. {ECO:0000269|PubMed:10527845}.
CC   -!- DEVELOPMENTAL STAGE: Expression is very low in embryonic epidermis at
CC       13.5 dpc and increases from 14.5 dpc to 16.5 dpc. In young mice
CC       expression increases in the testis of 2 to 6 weeks old animals, and
CC       then remains stable. {ECO:0000269|PubMed:10527845}.
CC   -!- MISCELLANEOUS: Antibodies against SPAG1 interfere with fertilization.
CC       {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC27944.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AF181252; AAF06160.1; -; mRNA.
DR   EMBL; AF181253; AAF06161.1; -; mRNA.
DR   EMBL; AK032601; BAC27944.1; ALT_INIT; mRNA.
DR   EMBL; BC046313; AAH46313.1; -; mRNA.
DR   CCDS; CCDS37061.1; -. [Q80ZX8-1]
DR   PIR; JC7111; JC7111.
DR   RefSeq; NP_036161.2; NM_012031.3. [Q80ZX8-1]
DR   RefSeq; XP_006520138.1; XM_006520075.2.
DR   RefSeq; XP_006520139.1; XM_006520076.3. [Q80ZX8-1]
DR   RefSeq; XP_006520141.1; XM_006520078.3. [Q80ZX8-3]
DR   RefSeq; XP_006520142.1; XM_006520079.3.
DR   RefSeq; XP_017172125.1; XM_017316636.1. [Q80ZX8-1]
DR   RefSeq; XP_017172126.1; XM_017316637.1.
DR   SMR; Q80ZX8; -.
DR   BioGrid; 205078; 1.
DR   STRING; 10090.ENSMUSP00000047335; -.
DR   iPTMnet; Q80ZX8; -.
DR   PhosphoSitePlus; Q80ZX8; -.
DR   EPD; Q80ZX8; -.
DR   PaxDb; Q80ZX8; -.
DR   PeptideAtlas; Q80ZX8; -.
DR   PRIDE; Q80ZX8; -.
DR   Ensembl; ENSMUST00000047348; ENSMUSP00000047335; ENSMUSG00000037617. [Q80ZX8-1]
DR   Ensembl; ENSMUST00000171205; ENSMUSP00000132233; ENSMUSG00000037617. [Q80ZX8-1]
DR   GeneID; 26942; -.
DR   KEGG; mmu:26942; -.
DR   UCSC; uc007vmn.1; mouse. [Q80ZX8-1]
DR   CTD; 6674; -.
DR   MGI; MGI:1349387; Spag1.
DR   eggNOG; KOG1124; Eukaryota.
DR   eggNOG; COG0457; LUCA.
DR   GeneTree; ENSGT00940000154697; -.
DR   HOGENOM; HOG000293399; -.
DR   InParanoid; Q80ZX8; -.
DR   KO; K19870; -.
DR   OMA; QYRKAYV; -.
DR   OrthoDB; 1070087at2759; -.
DR   PhylomeDB; Q80ZX8; -.
DR   TreeFam; TF106251; -.
DR   ChiTaRS; Spag1; mouse.
DR   PRO; PR:Q80ZX8; -.
DR   Proteomes; UP000000589; Chromosome 15.
DR   RNAct; Q80ZX8; protein.
DR   Bgee; ENSMUSG00000037617; Expressed in 191 organ(s), highest expression level in embryo.
DR   ExpressionAtlas; Q80ZX8; baseline and differential.
DR   Genevisible; Q80ZX8; MM.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0070286; P:axonemal dynein complex assembly; ISS:UniProtKB.
DR   GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 3.
DR   InterPro; IPR025986; RPAP3-like_C.
DR   InterPro; IPR013026; TPR-contain_dom.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR001440; TPR_1.
DR   InterPro; IPR019734; TPR_repeat.
DR   Pfam; PF13877; RPAP3_C; 1.
DR   Pfam; PF00515; TPR_1; 2.
DR   Pfam; PF13181; TPR_8; 1.
DR   SMART; SM00028; TPR; 8.
DR   SUPFAM; SSF48452; SSF48452; 3.
DR   PROSITE; PS50005; TPR; 6.
DR   PROSITE; PS50293; TPR_REGION; 4.
PE   1: Evidence at protein level;
DR   PRODOM; Q80ZX8.
DR   SWISS-2DPAGE; Q80ZX8.
KW   Alternative splicing; Cytoplasm; Fertilization; GTP-binding; Hydrolase;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; TPR repeat.
FT   CHAIN           1..901
FT                   /note="Sperm-associated antigen 1"
FT                   /id="PRO_0000106325"
FT   REPEAT          213..246
FT                   /note="TPR 1"
FT   REPEAT          247..279
FT                   /note="TPR 2"
FT   REPEAT          280..313
FT                   /note="TPR 3"
FT   REPEAT          430..464
FT                   /note="TPR 4"
FT   REPEAT          472..505
FT                   /note="TPR 5"
FT   REPEAT          507..539
FT                   /note="TPR 6"
FT   REPEAT          606..639
FT                   /note="TPR 7"
FT   REPEAT          640..673
FT                   /note="TPR 8"
FT   NP_BIND         756..763
FT                   /note="GTP"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         351
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:21183079"
FT   MOD_RES         359
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:21183079"
FT   MOD_RES         703
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U2X2"
FT   MOD_RES         739
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:21183079"
FT   MOD_RES         740
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:21183079"
FT   MOD_RES         766
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q07617"
FT   VAR_SEQ         1..372
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10527845"
FT                   /id="VSP_012684"
FT   VAR_SEQ         1..233
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_012685"
FT   CONFLICT        115
FT                   /note="C -> R (in Ref. 1; AAF06160)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        364
FT                   /note="E -> A (in Ref. 1; AAF06160)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        419
FT                   /note="H -> Y (in Ref. 1; AAF06160/AAF06161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        664
FT                   /note="E -> D (in Ref. 1; AAF06160/AAF06161)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   901 AA;  100670 MW;  C8D14100AD00D69F CRC64;
     MTAKAKDCPS LWGFGTTKTF KIPIEHLDFK YIENCSDVKH LEKILYVLRS GEEGYYPELT
     EFCEKCLTNL APKSRALRKD KPAETASSFS AEEWEKIDSD LKSWVSEIKR EENTCHFHDP
     ENHPGVEDPL PPVRGSTCCP HSGKETYSKS KTAKKRIPRD YAEWDKFDVE KECSKIDEDY
     KEKTVINNKA HLSKIETKIE TAGLTEKEKS FLANREKGKG NEAFYSGDYE EAVMYYTRSL
     SALPTAIAYN NRAQAEIKLQ RWSSALEDCE KALELDPGNV KALLRRATTY KHQNKLQEAV
     DDLRKVLQVE PDNDLAKKTL SEVERDLKNS EPVSELQTKG KRMVIEEVEN SGDEGGKGSA
     DEREDGGSDE AAMGNIQKKL MVRRSEGGRR SRRGRTPGPR AEQQGGLRET ATASTGDSHY
     PEEPRAADNP SGLKRRGNEL FRGGQFAEAA AQYSVAIAQL EPTGSANADE LSILYSNRAA
     CYLKEGNCRD CIQDCNRALE LHPFSVKPLL RRAMAYETLE QYRNAYVDYK TVLQIDCGIQ
     LASDSANRIA RILTELDGSK WRERLPPIPA VPTSEPLRVW LPAAETPDQD PCPNNCMPSI
     TDEKMFQALK EEGNQLVKDK NYKDAISKYN ECLKINSKAC AIYTNRALCY LKLGQFEEAK
     LDCEQALQID GENVKASHRL ALAQKGLENC RESGVDPSQV LLSPDSSEAA RHLDTKNDTA
     PPSKGRERRR IQVQEVDGSS DEEPERPAEA SATSAPARDG VEDGGSAEPA EKLDVSKPTN
     AYEFGQVLST ISARKDEEAC AHLLAITAPK DLPLLLSNKL EGDTFLLLIQ SLKSHLVAKD
     PSLVYEHLLY LSKAERFKTM LTLINKGQKE QMAQLFDGLS DTQSDGLTAE DVQALRRQYE
     L
//

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