(data stored in SCRATCH zone)

SWISSPROT: Q8GUN8_ARATH

ID   Q8GUN8_ARATH            Unreviewed;       309 AA.
AC   Q8GUN8;
DT   01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2003, sequence version 1.
DT   05-JUL-2017, entry version 114.
DE   RecName: Full=Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03162};
DE            EC=2.1.1.205 {ECO:0000256|HAMAP-Rule:MF_03162};
DE   AltName: Full=2'-O-ribose RNA methyltransferase TRM7 homolog {ECO:0000256|HAMAP-Rule:MF_03162};
GN   OrderedLocusNames=At5g01230 {ECO:0000313|Araport:AT5G01230,
GN   ECO:0000313|EMBL:AED90314.1};
GN   ORFNames=F7J8.210 {ECO:0000313|EMBL:AED90314.1}, F7J8_210
GN   {ECO:0000313|EMBL:AED90314.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000313|EMBL:AAN86163.1};
RN   [1] {ECO:0000313|EMBL:AED90314.1, ECO:0000313|Proteomes:UP000006548}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX   PubMed=11130714; DOI=10.1038/35048507;
RG   Kazusa DNA Research Institute;
RG   Cold Spring Harbor and Washington University in St Louis Sequencing Consortium;
RG   European Union Arabidopsis Genome Sequencing Consortium;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., See L.H., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Dusterhoft A., Stiekema W., Pohl T.,
RA   Entian K.D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Lankhorst R.K., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.W.,
RA   Bevan M., Fransz P.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2] {ECO:0000313|EMBL:AAN86163.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Yamada K., Chan M.M., Chang C.H., Dale J.M., Hsuan V.W., Lee J.M.,
RA   Onodera C.S., Quach H.L., Tang C.C., Toriumi M., Wong C., Wu H.C.,
RA   Yu G., Yuan S., Chen H., Cheuk R., Jones T., Kim C.J., Nguyen M.,
RA   Palm C.J., Shinn P., Southwick A., Tripp M.G., Wu T., Davis R.W.,
RA   Ecker J.R., Theologis A.;
RT   "Arabidopsis Open Reading Frame (ORF) Clones.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:AED90314.1}
RP   NUCLEOTIDE SEQUENCE.
RG   TAIR;
RA   Swarbreck D., Lamesch P., Wilks C., Huala E.;
RL   Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:AED90314.1, ECO:0000313|Proteomes:UP000006548}
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Methylates the 2'-O-ribose of nucleotides at positions
CC       32 and 34 of the tRNA anticodon loop of substrate tRNAs.
CC       {ECO:0000256|HAMAP-Rule:MF_03162}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine +
CC       cytidine(32)/guanosine(34) in tRNA = S-adenosyl-L-homocysteine +
CC       2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA.
CC       {ECO:0000256|HAMAP-Rule:MF_03162}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03162}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC       methyltransferase superfamily. RNA methyltransferase RlmE family.
CC       TRM7 subfamily. {ECO:0000256|HAMAP-Rule:MF_03162}.
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DR   EMBL; BT002330; AAN86163.1; -; mRNA.
DR   EMBL; CP002688; AED90314.1; -; Genomic_DNA.
DR   RefSeq; NP_851026.1; NM_180695.3.
DR   UniGene; At.26557; -.
DR   STRING; 3702.AT5G01230.1; -.
DR   EnsemblPlants; AT5G01230.1; AT5G01230.1; AT5G01230.
DR   GeneID; 830268; -.
DR   Gramene; AT5G01230.1; AT5G01230.1; AT5G01230.
DR   Araport; AT5G01230; -.
DR   TAIR; locus:2150069; AT5G01230.
DR   eggNOG; KOG1099; Eukaryota.
DR   eggNOG; COG0293; LUCA.
DR   OMA; FDIYKPP; -.
DR   OrthoDB; EOG09360F0Y; -.
DR   BioCyc; ARA:GQT-652-MONOMER; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q8GUN8; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IMP:TAIR.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0002181; P:cytoplasmic translation; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR   GO; GO:0030488; P:tRNA methylation; IMP:TAIR.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_01547; RNA_methyltr_E; 1.
DR   HAMAP; MF_03162; RNA_methyltr_E_TRM7; 1.
DR   InterPro; IPR028590; RNA_methyltr_E_Trm7.
DR   InterPro; IPR015507; rRNA-MeTfrase_E.
DR   InterPro; IPR002877; rRNA_MeTrfase_FtsJ_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   PANTHER; PTHR10920; PTHR10920; 1.
DR   Pfam; PF01728; FtsJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q8GUN8.
DR   SWISS-2DPAGE; Q8GUN8.
KW   Cell cycle {ECO:0000313|EMBL:AAN86163.1};
KW   Cell division {ECO:0000313|EMBL:AAN86163.1};
KW   Complete proteome {ECO:0000313|Proteomes:UP000006548};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03162};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_03162,
KW   ECO:0000313|EMBL:AED90314.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_03162};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_03162,
KW   ECO:0000313|EMBL:AED90314.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_03162}.
FT   DOMAIN       21    209       FtsJ. {ECO:0000259|Pfam:PF01728}.
FT   ACT_SITE    166    166       Proton acceptor. {ECO:0000256|HAMAP-Rule:
FT                                MF_03162}.
FT   BINDING      53     53       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000256|HAMAP-Rule:
FT                                MF_03162}.
FT   BINDING      55     55       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000256|HAMAP-Rule:
FT                                MF_03162}.
FT   BINDING      85     85       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_03162}.
FT   BINDING     101    101       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_03162}.
FT   BINDING     126    126       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_03162}.
SQ   SEQUENCE   309 AA;  33995 MW;  8E52FFC458B468BF CRC64;
     MGKASRDKRD IYYRKAKEEG WRARSAFKLL QIDEEFNIFE GVKRVVDLCA APGSWSQVLS
     RQLYLPAKSS AESKDGDLPL IVAIDLQPMA PIEGVIQVQG DITNARTAEV VIRHFDGCKA
     DLVVCDGAPD VTGLHDMDEF VQSQLILAGL TIVTHILKEG GKFIAKIFRG KDTSLLYCQL
     KLFFPTVTFA KPKSSRNSSI EAFAVCENYS PPEGFNPRDL HRLLEKVGSP SGGSDLDCSS
     GWLEGPNKVY IPFLACGDLT GYDSDRSYPL PREADGSSYQ SLDPIQPPIA PPYKRALELK
     KASAQSFNS
//

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