(data stored in SCRATCH zone)

SWISSPROT: Q8X957_ECO57

ID   Q8X957_ECO57            Unreviewed;       865 AA.
AC   Q8X957; Q7AHP7;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 125.
DE   RecName: Full=Aconitate hydratase B {ECO:0000256|PIRNR:PIRNR036687};
DE            EC=4.2.1.3 {ECO:0000256|PIRNR:PIRNR036687};
DE            EC=4.2.1.99 {ECO:0000256|PIRNR:PIRNR036687};
DE   AltName: Full=2-methylisocitrate dehydratase {ECO:0000256|PIRNR:PIRNR036687};
GN   Name=acnB {ECO:0000313|EMBL:AAG54422.1};
GN   OrderedLocusNames=ECs0122 {ECO:0000313|EMBL:BAB33545.1}, Z0128
GN   {ECO:0000313|EMBL:AAG54422.1};
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334 {ECO:0000313|Proteomes:UP000002519};
RN   [1] {ECO:0000313|EMBL:BAB33545.1, ECO:0000313|Proteomes:UP000000558}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC
RC   {ECO:0000313|Proteomes:UP000000558}, and Sakai
RC   {ECO:0000313|EMBL:BAB33545.1};
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [2] {ECO:0000313|EMBL:AAG54422.1, ECO:0000313|Proteomes:UP000002519}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EDL933 {ECO:0000313|EMBL:AAG54422.1}, and O157:H7 / EDL933 /
RC   ATCC 700927 / EHEC {ECO:0000313|Proteomes:UP000002519};
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G.III., Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
CC   -!- CATALYTIC ACTIVITY: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate =
CC       (Z)-but-2-ene-1,2,3-tricarboxylate + H(2)O.
CC       {ECO:0000256|PIRNR:PIRNR036687}.
CC   -!- CATALYTIC ACTIVITY: Citrate = isocitrate.
CC       {ECO:0000256|PIRNR:PIRNR036687}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|PIRSR:PIRSR036687-1};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit.
CC       {ECO:0000256|PIRSR:PIRSR036687-1};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC       isocitrate from oxaloacetate: step 2/2.
CC       {ECO:0000256|PIRNR:PIRNR036687}.
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC       {ECO:0000256|PIRNR:PIRNR036687}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AE005174; AAG54422.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33545.1; -; Genomic_DNA.
DR   PIR; B85495; B85495.
DR   PIR; B90644; B90644.
DR   RefSeq; NP_308149.1; NC_002695.1.
DR   RefSeq; WP_001303793.1; NZ_MWVM01000012.1.
DR   ProteinModelPortal; Q8X957; -.
DR   STRING; 155864.Z0128; -.
DR   EnsemblBacteria; AAG54422; AAG54422; Z0128.
DR   EnsemblBacteria; BAB33545; BAB33545; BAB33545.
DR   GeneID; 913670; -.
DR   KEGG; ece:Z0128; -.
DR   KEGG; ecs:ECs0122; -.
DR   PATRIC; fig|386585.9.peg.220; -.
DR   eggNOG; ENOG4107QIJ; Bacteria.
DR   eggNOG; COG1049; LUCA.
DR   HOGENOM; HOG000205991; -.
DR   KO; K01682; -.
DR   UniPathway; UPA00223; UER00718.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0047456; F:2-methylisocitrate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd01576; AcnB_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   Gene3D; 3.30.499.10; -; 2.
DR   Gene3D; 3.40.1060.10; -; 1.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR004406; Aconitase_B.
DR   InterPro; IPR015933; Aconitase_B_HEAT-like_dom.
DR   InterPro; IPR015929; Aconitase_B_swivel.
DR   PANTHER; PTHR43160:SF3; PTHR43160:SF3; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF06434; Aconitase_2_N; 1.
DR   Pfam; PF11791; Aconitase_B_N; 1.
DR   PIRSF; PIRSF036687; AcnB; 1.
DR   SUPFAM; SSF52016; SSF52016; 1.
DR   SUPFAM; SSF53732; SSF53732; 1.
DR   SUPFAM; SSF74778; SSF74778; 1.
DR   TIGRFAMs; TIGR00117; acnB; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8X957.
DR   SWISS-2DPAGE; Q8X957.
KW   4Fe-4S {ECO:0000256|PIRSR:PIRSR036687-1,
KW   ECO:0000256|SAAS:SAAS00640307};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000558,
KW   ECO:0000313|Proteomes:UP000002519};
KW   Iron {ECO:0000256|PIRSR:PIRSR036687-1};
KW   Iron-sulfur {ECO:0000256|PIRSR:PIRSR036687-1};
KW   Lyase {ECO:0000256|PIRNR:PIRNR036687, ECO:0000256|SAAS:SAAS00638284};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR036687-1};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR036687}.
FT   DOMAIN        4    156       Aconitase_B_N. {ECO:0000259|Pfam:
FT                                PF11791}.
FT   DOMAIN      168    382       Aconitase_2_N. {ECO:0000259|Pfam:
FT                                PF06434}.
FT   DOMAIN      472    818       Aconitase. {ECO:0000259|Pfam:PF00330}.
FT   REGION      244    246       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
FT   REGION      414    416       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
FT   METAL       710    710       Iron-sulfur (4Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR036687-1}.
FT   METAL       769    769       Iron-sulfur (4Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR036687-1}.
FT   METAL       772    772       Iron-sulfur (4Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR036687-1}.
FT   BINDING     191    191       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
FT   BINDING     498    498       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
FT   BINDING     791    791       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
FT   BINDING     796    796       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR036687-2}.
SQ   SEQUENCE   865 AA;  93484 MW;  0BFEDFB96F8BF6DE CRC64;
     MLEEYRKHVA ERAAEGIAPK PLDANQMAAL VELLKNPPAG EEEFLLDLLT NRVPPGVDEA
     AYVKAGFLAA VAKGEAKSPL LTPEKAIELL GTMQGGYNIH PLIDALDDAK LAPIAAKALS
     HTLLMFDNFY DVEEKAKAGN EYAKQVMQSW ADAEWFLNRP ALAEKLTVTV FKVTGETNTD
     DLSPAPDAWS RPDIPLHALA MLKNAREGIE PDQPGVVGPI KQIEALQQKG FPLAYVGDVV
     GTGSSRKSAT NSVLWFMGDD IPHVPNKRGG GLCLGGKIAP IFFNTMEDAG ALPIEVDVSN
     LNMGDVIDVY PYKGEVRNHE TGELLATFEL KTDVLIDEVR AGGRIPLIIG RGLTTKAREA
     LGLPHSDVFR QAKDVAESDR GFSLAQKMVG RACGVKGIRP GAYCEPKMTS VGSQDTTGPM
     TRDELKDLAC LGFSADLVMQ SFCHTAAYPK PVDVNTHHTL PDFIMNRGGV SLRPGDGVIH
     SWLNRMLLPD TVGTGGDSHT RFPIGISFPA GSGLVAFAAA TGVMPLDMPE SVLVRFKGKM
     QPGITLRDLV HAIPLYAIKQ GLLTVEKKGK KNIFSGRILE IEGLPDLKVE QAFELTDASA
     ERSAAGCTIK LNKEPIIEYL NSNIVLLKWM IAEGYGDRRT LERRIQGMEK WLANPELLEA
     DADAEYAAVI DIDLADIKEP ILCAPNDPDD ARPLSAVQGE KIDEVFIGSC MTNIGHFRAA
     GKLLDAHKGQ LPTRLWVAPP TRMDAAQLTE EGYYSVFGKS GARIEIPGCS LCMGNQARVA
     DGATVVSTST RNFPNRLGTG ANVFLASAEL AAVAALIGKL PTPEEYQTYV AQVDKTAVDT
     YRYLNFNQLS QYTEKADGVI FQTAV
//

If you have problems or comments...

PBIL Back to PBIL home page