(data stored in ACNUC9543 zone)

SWISSPROT: PRP16_SCHPO

ID   PRP16_SCHPO             Reviewed;        1173 AA.
AC   Q9P774; Q9UUE8;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2004, sequence version 2.
DT   08-MAY-2019, entry version 123.
DE   RecName: Full=Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16;
DE            EC=3.6.4.13;
GN   Name=prp16; ORFNames=SPBC1711.17, SPBC17G9.01;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Probable ATP-binding RNA helicase involved in pre-mRNA
CC       splicing. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. PRP16 sub-subfamily. {ECO:0000305}.
DR   EMBL; CU329671; CAB88247.1; -; Genomic_DNA.
DR   PIR; T39724; T39724.
DR   RefSeq; NP_595890.2; NM_001021796.3.
DR   SMR; Q9P774; -.
DR   BioGrid; 276550; 6.
DR   IntAct; Q9P774; 3.
DR   STRING; 4896.SPBC1711.17.1; -.
DR   MaxQB; Q9P774; -.
DR   PaxDb; Q9P774; -.
DR   PRIDE; Q9P774; -.
DR   EnsemblFungi; SPBC1711.17.1; SPBC1711.17.1:pep; SPBC1711.17.
DR   GeneID; 2540006; -.
DR   KEGG; spo:SPBC1711.17; -.
DR   EuPathDB; FungiDB:SPBC1711.17; -.
DR   PomBase; SPBC1711.17; prp16.
DR   HOGENOM; HOG000175261; -.
DR   InParanoid; Q9P774; -.
DR   KO; K12815; -.
DR   OMA; FIHTKAM; -.
DR   PhylomeDB; Q9P774; -.
DR   Reactome; R-SPO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   Reactome; R-SPO-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-SPO-72187; mRNA 3'-end processing.
DR   PRO; PR:Q9P774; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0000790; C:nuclear chromatin; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IDA:PomBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034459; F:ATP-dependent 3'-5' RNA helicase activity; IBA:GO_Central.
DR   GO; GO:0004004; F:ATP-dependent RNA helicase activity; ISO:PomBase.
DR   GO; GO:0003723; F:RNA binding; IDA:PomBase.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; ISO:PomBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd00079; HELICc; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR011709; DUF1605.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q9P774.
DR   SWISS-2DPAGE; Q9P774.
KW   ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN         1   1173       Pre-mRNA-splicing factor ATP-dependent
FT                                RNA helicase prp16.
FT                                /FTId=PRO_0000055149.
FT   DOMAIN      502    665       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      687    862       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     515    522       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       612    615       DEAH box.
SQ   SEQUENCE   1173 AA;  132971 MW;  1B382AC72F2C2249 CRC64;
     MLSHNQNDLL FTKLIDKLTD YMSSKDAATS LASRVLTIAK GSSSSTEFSN ALHTFGRFSD
     NDSVLIYDLC KSIIGLETNL GISKDKLPGN DGSQVAGLVL SNRSGLKGRE PKKSQLGLDV
     LAVQKKKEKS QVEGRSELSQ VENDSLESIG NYDRVEFKRP KNPHEKHFRP LRQRSSVDDH
     QEFESEDDKY RRNSYSSWSG SNFESSNGSN RRRYRTQMEE PLSSKRRNRF GNGSRRDVDS
     SKYSHDSDYS YGAHSSWDAR DVEYPEEDPE SKADRQRWEE EQAHLDRDWY MNSESQNLLG
     DEVHNPFSDF ETVEDRAHEA EFIEKQKKHL SIEASDRFKE NSMWEKNRMI TSGVSKAPGL
     ESDYSLMEER RVHLLVDELR PHFLDGAEFS SKKVGDITSV RDPQSDLAIN ARLGSRLVRE
     RREFRERQKA ASAATSLAGT SLGNVMGLKD SNDEDAKAGT TPVKVAGRSE QSNKKDTEFA
     RTKSYREQRE FLPAFAVREQ LLSVIRDNQV LIVVGETGSG KTTQLAQFLY EDGYHRNGMI
     GCTQPRRVAA MSVAKRVSEE MGVRLGSTVG YSIRFEDVTG PDTVIKYMTD GVLLRESLMQ
     NNLEKYSVII MDEAHERSLN TDILMGLLKK VLSRRRDIKL LVTSATMNSQ KFSDFFGGAP
     QFTIPGRTYP VDIMFAKAPC SDYVEAAVRQ VLQIHLSQPA GDILVFMTGQ EDIEATCEII
     ADRLNQLHDA PRLSILPIYS QMPADLQAKI FDSAEPGVRK VVVATNIAET SLTVHGISYV
     VDTGYCKLKM YNSKLGIDTL QVTPISQANA NQRAGRAGRT GPGIAYRLYT EMAYIREMFE
     TTLPEIQRTN LSNTVLILKS LGVEEISDFD FMDRPPNDTL MASLYELWTL GALDNFGKLT
     TLGKKMSLFP MDPSLSKLII IAEDYKCTEE IITIVSMLSV PSVFYRPKER AEESDAAREK
     FNVPESDHLM LLNIYQHWQR NGYSNSWCSK HFLHSKTLKR ARDIRQQLVE IMSKQKISLE
     SVSDWDIVRR VLCSAYFHQA ACAKGIGEYV HLRSGMPCHL HVTSSLYGLG YLPDYVIYHE
     LVLTSKEYMN IVTSVDPYWL AEFGGVYYSV KERFRNETES YDRVFSSKPQ LDAQIAADRE
     LDAKQKLAKN QEPVKSKRKS VIRAKPPRRV RGF
//

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