(data stored in ACNUC7421 zone)

HOGENOM: ARATH_4_PE3761

ID   ARATH_4_PE3761                       STANDARD;      PRT;   721 AA.
AC   ARATH_4_PE3761; Q9ZPI6;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Peroxisomal fatty acid beta-oxidation multifunctional
DE   protein AIM1;AltName: Full=Protein ABNORMAL INFLORESCENCE MERISTEM 1;
DE   Short=AtAIM1;Includes: RecName: Full=Enoyl-CoA
DE   hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
DE   EC=4.2.1.17; EC=5.1.2.3; EC=5.3.3.8;Includes: RecName:
DE   Full=3-hydroxyacyl-CoA dehydrogenase; EC=1.1.1 35; (ARATH_4.PE3761).
GN   Name=AIM1; OrderedLocusNames=At4g29010; ORFNames=F19B15.40;
OS   ARABIDOPSIS THALIANA.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots; rosids;
OC   eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ARATH_4.PE3761.
CC       Arabidopsis thaliana chromosome 4 TAIR10 full sequence 1..18585056
CC       annotated by Ensembl Genomes
CC   -!- ANNOTATIONS ORIGIN:AIM1_ARATH
CC   -!- FUNCTION: Involved in peroxisomal fatty acid beta-oxidation.
CC       Required for wound-induced jasmonate biosynthesis. Possesses
CC       enoyl-CoA hydratase activity against short chain substrates (C4-
CC       C6) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of
CC       variable sizes (C6-C16).
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-
CC       CoA.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=115 uM for crotonyl-CoA;
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBCELLULAR LOCATION: Peroxisome (Probable).
CC   -!- TISSUE SPECIFICITY: Widely expressed.
CC   -!- DOMAIN: The epimerase and isomerase activities are contained in
CC       the N-terminal region while the dehydrogenase activity is in the
CC       C-terminal region (By similarity).
CC   -!- DISRUPTION PHENOTYPE: Reduced rosette size, twisted leaves, and
CC       abnormal and sterile flowers.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-
CC       CoA dehydrogenase family.
CC   -!- GENE_FAMILY: HOG000261347 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Arabidopsis_thaliana;AT4G29010;AT4G29010.1;AT4G29010.1.
DR   EMBL; AF123253; - ;
DR   EMBL; AK220767; - ;
DR   EMBL; AK220989; - ;
DR   EMBL; AK221468; - ;
DR   EMBL; AL078470; - ;
DR   EMBL; AL161574; - ;
DR   EMBL; AY059815; - ;
DR   EMBL; AY072072; - ;
DR   EMBL; AY096659; - ;
DR   UniProtKB/Swiss-Prot; Q9ZPI6; -.
DR   EMBL; AF123253; AAD18041.1; -; Genomic_DNA.
DR   EMBL; AL078470; CAB43915.1; -; Genomic_DNA.
DR   EMBL; AL161574; CAB79659.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE85572.1; -; Genomic_DNA.
DR   EMBL; AY059815; AAL24297.1; -; mRNA.
DR   EMBL; AY072072; AAL59895.1; -; mRNA.
DR   EMBL; AY096659; AAM20293.1; -; mRNA.
DR   IPI; IPI00545226; -.
DR   PIR; T08956; T08956.
DR   RefSeq; NP_194630.1; NM_119045.4.
DR   UniGene; At.3404; -.
DR   UniGene; At.48915; -.
DR   HSSP; P00348; 3HDH.
DR   ProteinModelPortal; Q9ZPI6; -.
DR   SMR; Q9ZPI6; 6-715.
DR   STRING; Q9ZPI6; -.
DR   PRIDE; Q9ZPI6; -.
DR   ProMEX; Q9ZPI6; -.
DR   EnsemblPlants; AT4G29010.1; AT4G29010.1; AT4G29010.
DR   GeneID; 829022; -.
DR   GenomeReviews; CT486007_GR; AT4G29010.
DR   KEGG; ath:AT4G29010; -.
DR   NMPDR; fig|3702.1.peg.20868; -.
DR   TAIR; At4g29010; -.
DR   GeneTree; EPGT00070000028643; -.
DR   InParanoid; Q9ZPI6; -.
DR   OMA; KTASAQP; -.
DR   PhylomeDB; Q9ZPI6; -.
DR   ProtClustDB; CLSN2916032; -.
DR   Genevestigator; Q9ZPI6; -.
DR   GO; GO:0005618; C:cell wall; IDA:TAIR.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IDA:UniProtKB.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:EC.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:EC.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IDA:UniProtKB.
DR   GO; GO:0009908; P:flower development; IMP:TAIR.
DR   GO; GO:0009695; P:jasmonic acid biosynthetic process; IMP:UniProtKB.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH_C-like.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 2.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   SUPFAM; SSF48179; 6DGDH_C_like; 2.
DR   PROSITE; PS00067; 3HCDH; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
DR   HOGENOMDNA; ARATH_4.PE3761; -.
KW   AT4G29010pg.C_scaffold_1320000031; AT4G29010.1.C_scaffold_1320000031;
KW   Q56Y55; Q56ZH6; Q570E1; AF123253; AK220767; AK220989; AK221468; AL078470;
KW   AY059815; AY072072; AY096659;
KW   Complete proteome; Fatty acid metabolism; Isomerase; Lipid metabolism;
KW   Lyase; Multifunctional enzyme; NAD; Oxidoreductase; Peroxisome;
KW   Reference proteome.
SQ   SEQUENCE   721 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MAKKIGVTME VGNDGVAVIT ISNPPVNSLA SPIISGLKEK FRDANQRNDV KAIVLIGNNG
     RFSGGFDINV FQQVHKTGDL SLMPEVSVEL VCNLMEDSRK PVVAAVEGLA LGGGLELAMA
     CHARVAAPKA QLGLPELTLG VIPGFGGTQR LPRLVGLAKA TDMILLSKSI SSEEGHKLGL
     IDALVPPGDV LSTSRKWALD IAEGRKPFLQ SLHRTDKIGS LSEARAILKN SRQLAKKIAP
     NMPQHHACIE VIEEGIIHGG YSGVLKEAEV FKQLVLSDTA KGLVHVFFAQ RATSKVPNVT
     DVGLKPRPIK KVAVIGGGLM GSGIATALLL SNIRVVLKEI NSEFLMKGIK SVEANMKSLV
     SRGKLTQDKA GKALSLFKGV LDYTEFNDVD MVIEAVIENI QLKQNIFKEI EKVCSPHCIL
     ASNTSTIDLD VIGEKTNSKD RIVGAHFFSP AHLMPLLEIV RSKNTSAQVI LDLMAVGKAI
     KKVPVVVGNC IGFAVNRTFF PYSQAAHMLA NLGVDLFRID SVITSFGLPL GPFQLGDLAG
     HGIGLAVGPI YAKVYGDRMF RSPMTELLLK SGRNGKINGR GYYIYEKGSK PKPDPSVLSI
     VEKSRKLTNI MPGGKPISVT DKEIVEMILF PVVNEACRVL DEGVVIRASD LDIASVLGMS
     FPSYRGGIVF WADTVGPKYI YERLKKLSET YGSFFKPSRY LEERAMNGML LSESKSSRSK
     L
//

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