(data stored in ACNUC7421 zone)


ID   BACAH_1; SV 1; empty ; DNA; empty ; PRO; 55939 BP.
AC   NC_008598;
PR   Project: 58795;
DE   Bacillus thuringiensis str. Al Hakam plasmid pALH1, complete sequence.
OS   Bacillus thuringiensis str. Al Hakam
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus
OC   group.
RN   [1]
RP   1-55939
RX   PUBMED; 17337577.
RA   Challacombe,J.F., Altherr,M.R., Xie,G., Bhotika,S.S., Brown,N., Bruce,D.,
RA   Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C.,
RA   Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A.,
RA   Green,L.D., Han,C.S., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P.,
RA   Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., Martinez,D.,
RA   McMurry,K., Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C.,
RA   Okinaka,R.T., Parson-Quintana,B., Reilly,L.P., Richardson,P.,
RA   Robinson,D.L., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N.,
RA   Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Gilna,P. and
RA   Brettin,T.S.;
RT   "The complete genome sequence of Bacillus thuringiensis Al Hakam";
RL   J. Bacteriol. 189 (9), 3680-3681 (2007)
RN   [2]
RP   1-55939
RG   NCBI Genome Project
RT   "Direct Submission";
RL   Submitted (29-NOV-2006) National Center for Biotechnology Information,
RL   NIH, Bethesda, MD 20894, USA
RN   [3]
RP   1-55939
RA   Lucas,S., Pitluck,S., Chertkov,O., Goodwin,L.A., Han,l., Munk,A.C.,
RA   Saunders,E., Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P.,
RA   Martinez,A.D., Misra,M., Tapia,R., Thayer,N.N., Xie,G., Brown,N.,
RA   Green,L.D., Hill,K.K., Jackson,P.J., Longmire,J., Rubin,E. and
RA   Richardson,P.;
RT   "Direct Submission";
RL   Submitted (14-NOV-2006) Joint Genome Institute, Department of Energy, 2800
RL   Mitchell Drive, Walnut Creek, Ca 94598, USA
CC   PROVISIONAL REFSEQ: This record has not yet been subject to final
CC   NCBI review. The reference sequence was derived from CP000486.
CC   Bacillus thuringiensis Al Hakam was collected at a suspected
CC   bioweapons facility in Iraq by the United Nations Special
CC   Commission (Genome differences that distinguish Bacillus anthracis
CC   from Bacillus cereus and Bacillus thuringiensis. Radnedge, L,
CC   Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and
CC   Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and
CC   fosmid libraries were prepared at the Joint Genome Institute in Los
CC   Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI
CC   Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft
CC   assemblies were based on 246217 total reads. All libraries provided
CC   23x coverage of the genome.  The Phred/Phrap/Consed software
CC   package (www.phrap.com) was used for sequence assembly and quality
CC   assessment.  After the shotgun stage, reads were assembled with
CC   parallel phrap (High Performance Software, LLC). Possible
CC   mis-assemblies were corrected manually or by transposon bombing
CC   (Epicentre Biotechnologies) of bridging clones. Gaps between
CC   contigs were closed by editing in Consed, custom primer walk or PCR
CC   amplification. A total of 25321 additional reactions were necessary
CC   to close gaps and to raise the quality of the finished sequence.
CC   The completed genome sequence of B. thuringiensis Al Hakam achieves
CC   an average of 24-fold sequence coverage per base with error rate
CC   less than 1 in 100,000. Gene predictions were obtained using
CC   Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis
CC   of the gene predictions was performed by comparing coding sequences
CC   against the PFam, BLOCKS and Prodom databases. Gene definitions and
CC   functional classes were added manually by a team of annotators at
CC   JGI-LANL, using BLAST results in addition to information from the
CC   basic analysis.
CC   COMPLETENESS: full length.
FH   Key             Location/Qualifiers
FT   source          1..55939
FT                   /strain="Al Hakam"
FT                   /mol_type="genomic DNA"
FT                   /organism="Bacillus thuringiensis str. Al Hakam"
FT                   /plasmid="pALH1"
FT                   /db_xref="taxon:412694"
FT   gene            complement(665..1603)
FT                   /db_xref="GeneID:4542023"
FT                   /locus_tag="BALH_p0001"