(data stored in ACNUC7421 zone)
ID BACAH_2; SV 1; empty ; DNA; empty ; PRO; 5257091 BP.
PR Project: 58795;
DE Bacillus thuringiensis str. Al Hakam chromosome, complete genome.
OS Bacillus thuringiensis str. Al Hakam
OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus
RX PUBMED; 17337577.
RA Challacombe,J.F., Altherr,M.R., Xie,G., Bhotika,S.S., Brown,N., Bruce,D.,
RA Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C.,
RA Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A.,
RA Green,L.D., Han,C.S., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P.,
RA Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., Martinez,D.,
RA McMurry,K., Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C.,
RA Okinaka,R.T., Parson-Quintana,B., Reilly,L.P., Richardson,P.,
RA Robinson,D.L., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N.,
RA Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Gilna,P. and
RT "The complete genome sequence of Bacillus thuringiensis Al Hakam";
RL J. Bacteriol. 189 (9), 3680-3681 (2007)
RG NCBI Genome Project
RT "Direct Submission";
RL Submitted (28-NOV-2006) National Center for Biotechnology Information,
RL NIH, Bethesda, MD 20894, USA
RA Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T.,
RA Detter,J.C., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Goodwin,L.A.,
RA Han,l., Munk,A.C., Saunders,E., Brettin,T.S., Bruce,D., Challacombe,J.F.,
RA Gilna,P., Martinez,A.D., Misra,M., Tapia,R., Thayer,N.N., Xie,G.,
RA Brown,N., Green,L.D., Hill,K.K., Jackson,P.J., Longmire,J., Rubin,E. and
RT "Direct Submission";
RL Submitted (14-NOV-2006) Joint Genome Institute, Department of Energy, 2800
RL Mitchell Drive, Walnut Creek, Ca 94598, USA
CC PROVISIONAL REFSEQ: This record has not yet been subject to final
CC NCBI review. The reference sequence was derived from CP000485.
CC Bacillus thuringiensis Al Hakam was collected at a suspected
CC bioweapons facility in Iraq by the United Nations Special
CC Commission (Genome differences that distinguish Bacillus anthracis
CC from Bacillus cereus and Bacillus thuringiensis. Radnedge, L,
CC Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and
CC Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and
CC fosmid libraries were prepared at the Joint Genome Institute in Los
CC Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI
CC Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft
CC assemblies were based on 246217 total reads. All libraries provided
CC 23x coverage of the genome. The Phred/Phrap/Consed software
CC package (www.phrap.com) was used for sequence assembly and quality
CC assessment. After the shotgun stage, reads were assembled with
CC parallel phrap (High Performance Software, LLC). Possible
CC mis-assemblies were corrected manually or by transposon bombing
CC (Epicentre Biotechnologies) of bridging clones. Gaps between
CC contigs were closed by editing in Consed, custom primer walk or PCR
CC amplification. A total of 25321 additional reactions were necessary
CC to close gaps and to raise the quality of the finished sequence.
CC The completed genome sequence of B. thuringiensis Al Hakam achieves
CC an average of 24-fold sequence coverage per base with error rate
CC less than 1 in 100,000. Gene predictions were obtained using
CC Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis
CC of the gene predictions was performed by comparing coding sequences
CC against the PFam, BLOCKS and Prodom databases. Gene definitions and
CC functional classes were added manually by a team of annotators at
CC JGI-LANL, using BLAST results in addition to information from the
CC basic analysis.
CC COMPLETENESS: full length.
FH Key Location/Qualifiers
FT source 1..5257091
FT /strain="Al Hakam"
FT /mol_type="genomic DNA"
FT /organism="Bacillus thuringiensis str. Al Hakam"
FT gene 351..1748