(data stored in SCRATCH zone)


ID   BATHU1_1; SV 1; empty ; DNA; empty ; PRO; 5237682 BP.
AC   NC_005957;
PR   Project: 58089;
DE   Bacillus thuringiensis serovar konkukian str. 97-27 chromosome, complete
DE   genome.
OS   Bacillus thuringiensis serovar konkukian str. 97-27
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus
OC   group.
RN   [1]
RP   1-5237682
RX   PUBMED; 16621833.
RA   Han,C.S., Xie,G., Challacombe,J.F., Altherr,M.R., Bhotika,S.S., Brown,N.,
RA   Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C.,
RA   Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A.,
RA   Green,L.D., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P.,
RA   Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., McMurry,K.,
RA   Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T.,
RA   Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L., Rubin,E.,
RA   Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N., Thompson,L.S., Tice,H.,
RA   Ticknor,L.O., Wills,P.L., Brettin,T.S. and Gilna,P.;
RT   "Pathogenomic sequence analysis of Bacillus cereus and Bacillus
RT   thuringiensis isolates closely related to Bacillus anthracis";
RL   J. Bacteriol. 188 (9), 3382-3390 (2006)
RN   [2]
RP   1-5237682
RA   Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C., Hill,K.,
RA   Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S., Okinaka,R.,
RA   Richardson,P., Rubin,E. and Tice,H.;
RT   "Complete genome sequence of Bacillus thuringiensis 97-27";
RL   Unpublished
RN   [3]
RP   1-5237682
RG   NCBI Genome Project
RT   "Direct Submission";
RL   Submitted (11-SEP-2004) National Center for Biotechnology Information,
RL   NIH, Bethesda, MD 20894, USA
RN   [4]
RP   1-5237682
RA   Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C., Hill,K.,
RA   Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S., Okinaka,R.,
RA   Richardson,P., Rubin,E. and Tice,H.;
RT   "Direct Submission";
RL   Submitted (07-JUN-2004) Joint Genome Institute, Department of Energy, 2800
RL   Mitchell Drive, Walnut Creek, CA 94598, USA
CC   PROVISIONAL REFSEQ: This record has not yet been subject to final
CC   NCBI review. The reference sequence was derived from AE017355.
CC   Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was
CC   originally isolated from a case of severe human tissue necrosis
CC   (Bacillus thuringiensis subsp. konkukian (serotype H34)
CC   superinfection: Case report and experimental evidence of
CC   pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F,
CC   Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998
CC   36(7):2138-2139). B. thuringiensis is indigenous to many habitats
CC   worldwide; these include soil, insects, deciduous and coniferous
CC   leaves (Prediction of insecticidal activity of Bacillus
CC   thuringiensis strains by polymerase chain reaction product
CC   profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and
CC   Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B.
CC   thuringiensis is an insect pathogen that is widely used as a
CC   biopesticide in commercial agriculture. Infection of humans is
CC   unusual. The apparent pathogenic properties of B. thuringiensis
CC   97-27 are very unusual for B. thuringiensis; unlike most B.
CC   thuringiensis isolates, this isolate is very closely related to B.
CC   anthracis based on phylogenetic analysis (Fluorescent amplified
CC   fragment length polymorphism analysis of Bacillus anthracis,
CC   Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK,
CC   Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M,
CC   Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD,
CC   Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ
CC   Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were
CC   prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),
CC   NM. Shotgun sequencing was performed at the JGI Production Genomics
CC   Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x.
CC   Finishing was performed at JGI-LANL starting with 83 contigs and 16
CC   scaffolds. Repetitive regions were identified, assembled and
CC   finished by manually checking paired reads close to each repeat in
CC   the assembly with consed and then making a subassembly for each
CC   repetitive region. Fifty five gaps were closed with primer walks
CC   and 16 by PCR.Gene predictions were obtained using Glimmer and
CC   tRNAs were identified using tRNAScan-SE. Basic analysis of the gene
CC   predictions was performed by comparing coding sequences against the
CC   PFam, BLOCKS and Prodom databases. Gene definitions and functional
CC   classes were added manually by a team of annotators at JGI-LANL,
CC   using BLAST results in addition to information from the basic
CC   analysis. A total of 5540 features have been annotated on the
CC   sequence record.
CC   COMPLETENESS: full length.
FH   Key             Location/Qualifiers
FT   source          1..5237682
FT                   /strain="97-27"
FT                   /serovar="konkukian"
FT                   /mol_type="genomic DNA"
FT                   /organism="Bacillus thuringiensis serovar konkukian str.
FT                   97-27"
FT                   /db_xref="taxon:281309"
FT   gene            409..1749
FT                   /db_xref="GeneID:2857048"
FT                   /locus_tag="BT9727_0001"