(data stored in ACNUC7421 zone)

HOGENOM: ERWAC_1_PE14

ID   ERWAC_1_PE14                         STANDARD;      PRT;   252 AA.
AC   ERWAC_1_PE14; D4I4N9;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Thiazole synthase; EC=4.-.- -; (ERWAC_1.PE14).
GN   Name=thiG; OrderedLocusNames=EAMY_3725;
OS   ERWINIA AMYLOVORA CFBP1430.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Erwinia.
OX   NCBI_TaxID=665029;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ERWAC_1.PE14.
CC       Erwinia amylovora CFBP1430 plasmid pEA29, complete sequence.
CC       1..42673 annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:D4I4N9_ERWAC
CC   -!- FUNCTION: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-
CC       phosphate (DXP) to produce the thiazole phosphate moiety of
CC       thiamine. Sulfur is provided by the thiocarboxylate moiety of the
CC       carrier protein ThiS. In vitro, sulfur can be provided by H(2)S
CC       (By similarity).
CC   -!- CATALYTIC ACTIVITY: 1-deoxy-D-xylulose 5-phosphate +
CC       dehydroglycine + [ThiS]-COSH = 4-methyl-5-(2-
CC       phosphonooxyethyl)thiazole + [ThiS]-COOH + CO(2) + 2 H(2)O.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC   -!- SUBUNIT: Homotetramer. Forms heterodimers with either ThiH or ThiS
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the thiG family.
CC   -!- GENE_FAMILY: HOG000248049 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; D4I4N9; -.
DR   EMBL; FN434114; CBA18926.1; -; Genomic_DNA.
DR   RefSeq; YP_003522546.1; NC_013957.1.
DR   ProteinModelPortal; D4I4N9; -.
DR   GeneID; 8893800; -.
DR   GenomeReviews; FN434114_GR; EAMY_3725.
DR   KEGG; eam:EAMY_3725; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:HAMAP.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00443; ThiG; 1; -.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR008867; ThiG.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF05690; ThiG; 1.
DR   SUPFAM; SSF110399; ThiG; 1.
DR   HOGENOMDNA; ERWAC_1.PE14; -.
KW   Thiazole biosynthesis protein thiG;
KW   Complete proteome; Cytoplasm; Lyase; Oxidoreductase; Plasmid;
KW   Schiff base; Thiamine biosynthesis.
SQ   SEQUENCE   252 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MFYDFVPQSR FLLGTAGYPS PQILQQAIEA SGSEIITVSL RREGSQGGAF RALLTQLNKR
     VLPNTAGCHT VKEAVTTAYM ARELFNTRWI KLEVIGHADT LQPDPFALVE AARILCADGF
     QVFPYTTEDL ILGEKLLEAG CKLLMPWGAP IGSGQGLRNI EGLRSMRSWF KDIPLIIDAG
     IGAPSQAAQA MEMGFDGILL NTAVARAQDP LGMAQAFASA IRAGYDARSA GLIERRDMAT
     ASTPIFGMAQ FS
//

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