(data stored in ACNUC7421 zone)

HOGENOM: GARV4_1_PE1010

ID   GARV4_1_PE1010                       STANDARD;      PRT;   610 AA.
AC   GARV4_1_PE1010; D2RBU7;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Prolyl-tRNA synthetase; EC=6.1.1 15;AltName:
DE   Full=Proline--tRNA ligase; (GARV4_1.PE1010).
GN   Name=proS; OrderedLocusNames=HMPREF0424_1079;
OS   GARDNERELLA VAGINALIS 409-05.
OC   Bacteria; Actinobacteria; Actinobacteridae; Bifidobacteriales;
OC   Bifidobacteriaceae; Gardnerella.
OX   NCBI_TaxID=553190;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS GARV4_1.PE1010.
CC       Gardnerella vaginalis 409-05 chromosome, complete genome.
CC       genome.
CC   -!- ANNOTATIONS ORIGIN:D2RBU7_GARV4
CC   -!- FUNCTION: Catalyzes the attachment of proline to tRNA(Pro) in a
CC       two-step reaction: proline is first activated by ATP to form Pro-
CC       AMP and then transferred to the acceptor end of tRNA(Pro). As
CC       ProRS can inadvertently accommodate and process non-cognate amino
CC       acids such as alanine and cysteine, to avoid such errors it has
CC       two additional distinct editing activities against alanine. One
CC       activity is designated as 'pretransfer' editing and involves the
CC       tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other
CC       activity is designated 'posttransfer' editing and involves
CC       deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-
CC       tRNA(Pro) is not edited by ProRS (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-proline + tRNA(Pro) = AMP +
CC       diphosphate + L-prolyl-tRNA(Pro).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- DOMAIN: Consists of three domains: the N-terminal catalytic
CC       domain, the editing domain and the C-terminal anticodon-binding
CC       domain (By similarity).
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family. ProS type 1 subfamily.
CC   -!- GENE_FAMILY: HOG000076893 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; D2RBU7; -.
DR   EMBL; CP001849; ADB13647.1; -; Genomic_DNA.
DR   RefSeq; YP_003374282.1; NC_013721.1.
DR   GeneID; 8709380; -.
DR   GenomeReviews; CP001849_GR; HMPREF0424_1079.
DR   KEGG; gva:HMPREF0424_1079; -.
DR   OMA; IQPAELW; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004827; F:proline-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0006433; P:prolyl-tRNA aminoacylation; IEA:HAMAP.
DR   HAMAP; MF_01569; Pro_tRNA_synth_type1; 1; -.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-dom.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR002316; Pro-tRNA-synth_IIa.
DR   InterPro; IPR004500; Pro-tRNA-synth_IIa_bac-type.
DR   InterPro; IPR023717; Pro-tRNA-Synthase_IIa_type1.
DR   InterPro; IPR007214; YbaK/aa-tRNA-synth-assoc-dom.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF04073; YbaK; 1.
DR   PRINTS; PR01046; TRNASYNTHPRO.
DR   SUPFAM; SSF52954; Anticodon_bd; 1.
DR   SUPFAM; SSF55826; YbaK/aa-tRNA-synth-assoc-reg; 1.
DR   TIGRFAMs; TIGR00409; ProS_fam_II; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   HOGENOMDNA; GARV4_1.PE1010; -.
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis.
SQ   SEQUENCE   610 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MNSNVLRMSQ LFLRTLREDP ADADVTSAKL MQRAGYIRKS APGVWTWLPL GLKVLNKVQA
     IIRDEINGIG AQEVHFPALL PREPYEATNR WEEYGDNIFR LKDRHQADYL LAPTHEEVFT
     LLVKDMYSSY KDLPVTLYQI QTKYRDEFRP RAGLIRGREF IMQDGYSFSI DEDGLKSAYV
     EERAAYARIF DRLGIKYVIV HAVSGPMGGS DSEEFLAPMP IGEDTFALAP SGKAWNVEAL
     TTPEMQDVDY SATPAAQSLE TPDAKTIEAL VKVSNDLHPR KDGREWQADD TLKNLIIAVK
     HPADAEGGEH EEPWREIVAI GIPGDRQVDM KRLEAQFAPA EIEEATEDDL KSHPEFVKGY
     IGFSVLGPQA RKEGRGIANP VRYLMDAHVA KGSAWITGAD EDGKHVYNAV YGRDFEADGV
     VEAAQVRDGD MSPDGSGPLS FERGVEIGQV FQLGLKYSKA LGLSVLNENG KAVPVWMGCY
     GIGVSRVIAC LAETHHDDAG LAWPMAIAPA QVHVVATGKD QVAFDAAEKV VEELSKQGIE
     VIFDDRPKVS PGVKFKDAEL VGVPLIAVAG RDTVNNGTIE IRDRNGSNVE AVPVDEAAAR
     IVERVSELLA
//

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