(data stored in ACNUC7421 zone)

HOGENOM: GEOS0_1_PE1013

ID   GEOS0_1_PE1013                       STANDARD;      PRT;   609 AA.
AC   GEOS0_1_PE1013; E3IGL6;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Elongation factor 4; Short=EF-4; EC=3.6.5 n1;AltName:
DE   Full=Ribosomal back-translocase LepA; (GEOS0_1.PE1013).
GN   Name=lepA; OrderedLocusNames=GY4MC1_1057;
OS   GEOBACILLUS SP. Y4.1MC1.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=581103;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS GEOS0_1.PE1013.
CC       Geobacillus sp. Y4.1MC1 chromosome, complete genome.
CC       Ensembl
CC   -!- ANNOTATIONS ORIGIN:E3IGL6_GEOS0
CC   -!- FUNCTION: Required for accurate and efficient protein synthesis
CC       under certain stress conditions. May act as a fidelity factor of
CC       the translation reaction, by catalyzing a one-codon backward
CC       translocation of tRNAs on improperly translocated ribosomes. Back-
CC       translocation proceeds from a post-translocation (POST) complex to
CC       a pre-translocation (PRE) complex, thus giving elongation factor G
CC       a second chance to translocate the tRNAs correctly. Binds to
CC       ribosomes in a GTP-dependent manner (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + H(2)O = GDP + phosphate.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC       Cytoplasmic side (By similarity).
CC   -!- SIMILARITY: Belongs to the GTP-binding elongation factor family.
CC       LepA subfamily.
CC   -!- GENE_FAMILY: HOG000020624 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; E3IGL6; -.
DR   EMBL; CP002293; ADP73869.1; -; Genomic_DNA.
DR   RefSeq; YP_003988480.1; NC_014650.1.
DR   ProteinModelPortal; E3IGL6; -.
DR   GeneID; 9927925; -.
DR   GenomeReviews; CP002293_GR; GY4MC1_1057.
DR   KEGG; gmc:GY4MC1_1057; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR   GO; GO:0003924; F:GTPase activity; IEA:HAMAP.
DR   GO; GO:0043022; F:ribosome binding; IEA:HAMAP.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:HAMAP.
DR   GO; GO:0045727; P:positive regulation of translation; IEA:HAMAP.
DR   HAMAP; MF_00071; LepA; 1; -.
DR   InterPro; IPR009022; Elongation_fac_G/III/V.
DR   InterPro; IPR006297; LepA_GTP-bd.
DR   InterPro; IPR013842; LepA_GTP-bd_C.
DR   InterPro; IPR000795; ProtSyn_GTP-bd.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000640; Transl_elong_EFG/EF2_C.
DR   InterPro; IPR004161; Transl_elong_EFTu/EF1A_2.
DR   InterPro; IPR009000; Transl_elong_init/rib_B-barrel.
DR   Gene3D; G3DSA:3.30.70.240; Transl_elong_EFG/EF2_C; 1.
DR   Pfam; PF00679; EFG_C; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF06421; LepA_C; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SMART; SM00838; EFG_C; 1.
DR   SUPFAM; SSF54980; EFG_III_V; 2.
DR   SUPFAM; SSF50447; Translat_factor; 1.
DR   TIGRFAMs; TIGR01393; LepA; 1.
DR   TIGRFAMs; TIGR00231; Small_GTP; 1.
DR   PROSITE; PS00301; EFACTOR_GTP; 1.
DR   HOGENOMDNA; GEOS0_1.PE1013; -.
KW   GTP-binding protein LepA;
KW   Cell membrane; Complete proteome; GTP-binding; Hydrolase; Membrane;
KW   Nucleotide-binding; Protein biosynthesis.
SQ   SEQUENCE   609 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MNRDERRRRR ERIRNFSIIA HIDHGKSTLA DRILEKTGAL SEREMKEQML DSMELERERG
     ITIKLNAVQL KYKAKNGEEY IFHLIDTPGH VDFTYEVSRS LAACEGAILV VDAAQGIEAQ
     TLANVYLALD NDLEILPVIN KIDLPSADPE RVRREIEEVI GLDASEAVLA SAKVGIGIEE
     ILEKIVEKIP APSGDPDAPL KALIFDSHYD SYRGVVAYIR VVDGTVKPGQ KIKMMSTGKE
     FEVVEVGVFT PKPKQVDELT VGDVGYLTAS IKNVSDTRVG DTITDAENPA TEPLPGYRKL
     NPMVFCGMYP IDTARYNDLR EALEKLQLND AALHFEPETS QALGFGFRCG FLGLLHMEII
     QERIEREFNI DLITTAPSVV YKVFLTDGTE VQVDNPSNMP EPQKIDHIEE PYVKATIMVP
     NDYVGPVMEL CQGKRGNFVD MQYLDEKRVT LIYEIPLSEI VYDFFDTLKS STKGYASFDY
     ELIGYKPSNL VKMDILLNGE KIDALSFIVH RDSAYERGKV IVEKLKDLIP RQQFEVPVQA
     AIGNKIIARS TIKALRKNVL AKCYGGDVSR KRKLLEKQKE GKKRMKQIGS VEVPQEAFMA
     ILKIDDNKK
//

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