(data stored in ACNUC14577 zone)

HOGENOM: HS17_PE2949

ID   HS17_PE2949                          STANDARD;      PRT;   723 AA.
AC   HS17_PE2949; Q9NZ52; Q15017; Q6IS16; Q9UJY3;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=ADP-ribosylation factor-binding protein GGA3;AltName:
DE   Full=Golgi-localized, gamma ear-containing, ARF-binding protein 3;
DE   (HS17.PE2949).
GN   Name=GGA3; Synonyms=KIAA0154;
OS   HOMO SAPIENS.
OC   Eukaryota; Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia;
OC   Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi;
OC   Sarcopterygii; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;
OC   Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini;
OC   Hominoidea; Hominidae; Homininae; Homo.
OX   NCBI_TaxID=9606;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS HS17.PE2949.
CC       Homo sapiens chromosome 17 GRCh37  sequence 1..81195210 annotated by
CC       Ensembl
CC   -!- ANNOTATIONS ORIGIN:GGA3_HUMAN
CC   -!- FUNCTION: Plays a role in protein sorting and trafficking between
CC       the trans-Golgi network (TGN) and endosomes. Mediates the ARF-
CC       dependent recruitment of clathrin to the TGN and binds
CC       ubiquitinated proteins and membrane cargo molecules with a
CC       cytosolic acidic cluster-dileucine (AC-LL) motif.
CC   -!- SUBUNIT: Monomer. Interacts with SORT1, SORL1, LRP3, GGA binding
CC       partner (GGABP) and P200 (By similarity). Interacts with GGA1 and
CC       GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and
CC       RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR,
CC       IGF2R/CI-MPR and BACE1 and the accessory proteins SYNRG, EPN4,
CC       NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and
CC       UBC.
CC   -!- INTERACTION:
CC       Q15276:RABEP1; NbExp=4; IntAct=EBI-447404, EBI-447043;
CC       P0CG47:UBB; NbExp=2; IntAct=EBI-447404, EBI-413034;
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC       membrane; Peripheral membrane protein. Endosome membrane;
CC       Peripheral membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Long;
CC         IsoId=Q9NZ52-1; Sequence=Displayed;
CC       Name=Short;
CC         IsoId=Q9NZ52-2; Sequence=VSP_001745;
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC   -!- DOMAIN: The VHS domain functions as a recognition module for
CC       sorting signals composed of an acidic cluster followed by two
CC       leucines (AC-LL motif).
CC   -!- DOMAIN: The GAT domain is responsible for interaction with ARF-
CC       GTP, UBC and RABEP1. Required for recruitment to the TGN it
CC       prevents ARF-GTP hydrolysis.
CC   -!- DOMAIN: The unstructured hinge region contains clathrin-binding
CC       and an autoinhibitory (AC-LL) motifs.
CC   -!- DOMAIN: The GAE domain binds accessory proteins regulating GGAs
CC       function.
CC   -!- PTM: Phosphorylated by CK2 and dephosphorylated by PP2A (By
CC       similarity). Phosphorylation of GGA3 allows the internal AC-LL
CC       motif to bind the VHS domain and to inhibit the recognition of
CC       cargo signals.
CC   -!- PTM: Ubiquitinated.
CC   -!- SIMILARITY: Belongs to the GGA protein family.
CC   -!- SIMILARITY: Contains 1 GAE domain.
CC   -!- SIMILARITY: Contains 1 GAT domain.
CC   -!- SIMILARITY: Contains 1 VHS domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA09926.1; Type=Erroneous initiation;
CC   -!- GENE_FAMILY: HOG000231169 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Homo_sapiens;ENSG00000125447;ENST00000245541;ENSP00000245541.
DR   EMBL; AF190864; - ;
DR   EMBL; AF219138; - ;
DR   EMBL; AF219139; - ;
DR   EMBL; AK291651; - ;
DR   EMBL; AK293291; - ;
DR   EMBL; AK296843; - ;
DR   EMBL; AK301895; - ;
DR   EMBL; AK302278; - ;
DR   EMBL; AK302423; - ;
DR   EMBL; BC070044; - ;
DR   EMBL; D63876; - ;
DR   UniProtKB/Swiss-Prot; Q9NZ52; Q15017; Q6IS16; Q9UJY3; -.
DR   EMBL; AF219138; AAF42848.1; -; mRNA.
DR   EMBL; AF190864; AAF05709.1; -; mRNA.
DR   EMBL; AF219139; AAF42849.1; -; mRNA.
DR   EMBL; D63876; BAA09926.1; ALT_INIT; mRNA.
DR   EMBL; BC070044; AAH70044.1; -; mRNA.
DR   IPI; IPI00021174; -.
DR   IPI; IPI00219305; -.
DR   RefSeq; NP_001166174.1; NM_001172703.1.
DR   RefSeq; NP_054720.1; NM_014001.3.
DR   RefSeq; NP_619525.1; NM_138619.2.
DR   UniGene; Hs.87726; -.
DR   PDB; 1JPL; X-ray; 2.40 A; A/B/C/D=1-166.
DR   PDB; 1JUQ; X-ray; 2.20 A; A/B/C/D=1-166.
DR   PDB; 1LF8; X-ray; 2.30 A; A/B/C/D=1-166.
DR   PDB; 1P4U; X-ray; 2.20 A; A=571-723.
DR   PDB; 1WR6; X-ray; 2.60 A; A/B/C/D=209-319.
DR   PDB; 1YD8; X-ray; 2.80 A; G/H=208-301.
DR   PDBsum; 1JPL; -.
DR   PDBsum; 1JUQ; -.
DR   PDBsum; 1LF8; -.
DR   PDBsum; 1P4U; -.
DR   PDBsum; 1WR6; -.
DR   PDBsum; 1YD8; -.
DR   ProteinModelPortal; Q9NZ52; -.
DR   SMR; Q9NZ52; 1-157, 168-300, 579-723.
DR   IntAct; Q9NZ52; 4.
DR   MINT; MINT-126320; -.
DR   STRING; Q9NZ52; -.
DR   PhosphoSite; Q9NZ52; -.
DR   PRIDE; Q9NZ52; -.
DR   Ensembl; ENST00000245541; ENSP00000245541; ENSG00000125447.
DR   GeneID; 23163; -.
DR   KEGG; hsa:23163; -.
DR   UCSC; uc002jni.1; human.
DR   UCSC; uc002jnj.1; human.
DR   CTD; 23163; -.
DR   GeneCards; GC17M068642; -.
DR   H-InvDB; HIX0014168; -.
DR   HGNC; HGNC:17079; GGA3.
DR   HPA; HPA022945; -.
DR   MIM; 606006; gene.
DR   neXtProt; NX_Q9NZ52; -.
DR   eggNOG; prNOG11451; -.
DR   GeneTree; ENSGT00600000084107; -.
DR   InParanoid; Q9NZ52; -.
DR   OMA; QGSPMKG; -.
DR   OrthoDB; EOG4W0XCS; -.
DR   PhylomeDB; Q9NZ52; -.
DR   Pathway_Interaction_DB; arf_3pathway; Arf1 pathway.
DR   NextBio; 44545; -.
DR   ArrayExpress; Q9NZ52; -.
DR   Bgee; Q9NZ52; -.
DR   CleanEx; HS_GGA3; -.
DR   Genevestigator; Q9NZ52; -.
DR   GermOnline; ENSG00000125447; Homo sapiens.
DR   GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
DR   GO; GO:0030306; F:ADP-ribosylation factor binding; IDA:UniProtKB.
DR   GO; GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR   InterPro; IPR013041; Clathrin/coatomer_app_Ig-like.
DR   InterPro; IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
DR   InterPro; IPR008153; Clathrin_g-adaptin_app.
DR   InterPro; IPR008942; ENTH_VHS.
DR   InterPro; IPR004152; GAT.
DR   InterPro; IPR002014; VHS.
DR   InterPro; IPR018205; VHS_subgr.
DR   Gene3D; G3DSA:2.60.40.1230; Clathrin_g-adaptin_app; 1.
DR   Gene3D; G3DSA:1.25.40.90; ENTH_VHS; 1.
DR   Pfam; PF02883; Alpha_adaptinC2; 1.
DR   Pfam; PF03127; GAT; 1.
DR   Pfam; PF00790; VHS; 1.
DR   SMART; SM00809; Alpha_adaptinC2; 1.
DR   SMART; SM00288; VHS; 1.
DR   SUPFAM; SSF49348; Clath_adapt; 1.
DR   SUPFAM; SSF48464; ENTH_VHS; 1.
DR   PROSITE; PS50180; GAE; 1.
DR   PROSITE; PS50909; GAT; 1.
DR   PROSITE; PS50179; VHS; 1.
DR   HOGENOMDNA; HS17.PE2949; -.
KW   ENSG000001254471755old_1320000031; ENSP000002455417901old_1320000031;
KW   A8K6I6_HUMAN; B7Z1E9_HUMAN; B7Z456_HUMAN; B7Z7E2_HUMAN; B7Z7M9_HUMAN;
KW   AF190864; AF219138; AF219139; AK291651; AK293291; AK296843; AK301895;
KW   AK302423; BC070044; D63876;
KW   3D-structure; Alternative splicing; Complete proteome; Endosome;
KW   Golgi apparatus; Membrane; Phosphoprotein; Polymorphism;
KW   Protein transport; Reference proteome; Transport; Ubl conjugation.
SQ   SEQUENCE   723 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL AHKIQSPQEW
     EALQALTVLE ACMKNCGRRF HNEVGKFRFL NELIKVVSPK YLGDRVSEKV KTKVIELLYS
     WTMALPEEAK IKDAYHMLKR QGIVQSDPPI PVDRTLIPSP PPRPKNPVFD DEEKSKLLAK
     LLKSKNPDDL QEANKLIKSM VKEDEARIQK VTKRLHTLEE VNNNVRLLSE MLLHYSQEDS
     SDGDRELMKE LFDQCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ
     VINGEVATLT LPDSEGNSQC SNQGTLIDLA ELDTTNSLSS VLAPAPTPPS SGIPILPPPP
     QASGPPRSRS SSQAEATLGP SSTSNALSWL DEELLCLGLA DPAPNVPPKE SAGNSQWHLL
     QREQSDLDFF SPRPGTAACG ASDAPLLQPS APSSSSSQAP LPPPFPAPVV PASVPAPSAG
     SSLFSTGVAP ALAPKVEPAV PGHHGLALGN SALHHLDALD QLLEEAKVTS GLVKPTTSPL
     IPTTTPARPL LPFSTGPGSP LFQPLSFQSQ GSPPKGPELS LASIHVPLES IKPSSALPVT
     AYDKNGFRIL FHFAKECPPG RPDVLVVVVS MLNTAPLPVK SIVLQAAVPK SMKVKLQPPS
     GTELSPFSPI QPPAAITQVM LLANPLKEKV RLRYKLTFAL GEQLSTEVGE VDQFPPVEQW
     GNL
//

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