(data stored in ACNUC10043 zone)

HOGENOM: METP4_1_PE1013

ID   METP4_1_PE1013                       STANDARD;      PRT;   391 AA.
AC   METP4_1_PE1013; E1RKE1;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Probable tRNA sulfurtransferase; EC=2.8.1 4;AltName:
DE   Full=Sulfur carrier protein ThiS sulfurtransferase;AltName: Full=Thiamine
DE   biosynthesis protein thiI;AltName: Full=tRNA 4-thiouridine synthase;
DE   (METP4_1.PE1013).
GN   Name=thiI; OrderedLocusNames=Mpet_1027;
OS   METHANOPLANUS PETROLEARIUS DSM 11571.
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC   Methanomicrobiaceae; Methanoplanus.
OX   NCBI_TaxID=679926;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS METP4_1.PE1013.
CC       Methanoplanus petrolearius DSM 11571 chromosome, complete genome.
CC       sequence.
CC   -!- ANNOTATIONS ORIGIN:E1RKE1_METP4
CC   -!- FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA
CC       to produce 4-thiouridine in position 8 of tRNAs, which functions
CC       as a near-UV photosensor. Also catalyzes the transfer of sulfur to
CC       the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate.
CC       This is a step in the synthesis of thiazole, in the thiamine
CC       biosynthesis pathway. The sulfur is donated as persulfide by iscS
CC       (By similarity).
CC   -!- CATALYTIC ACTIVITY: L-cysteine + 'activated' tRNA = L-serine +
CC       tRNA containing a thionucleotide.
CC   -!- CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH +
CC       [ThiS]-COSH + AMP.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the thiI family.
CC   -!- SIMILARITY: Contains 1 THUMP domain.
CC   -!- GENE_FAMILY: HOG000227470 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; E1RKE1; -.
DR   EMBL; CP002117; ADN35794.1; -; Genomic_DNA.
DR   RefSeq; YP_003894232.1; NC_014507.1.
DR   GeneID; 9743491; -.
DR   GenomeReviews; CP002117_GR; Mpet_1027.
DR   KEGG; mpi:Mpet_1027; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0016783; F:sulfurtransferase activity; IEA:HAMAP.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:HAMAP.
DR   GO; GO:0034227; P:tRNA thio-modification; IEA:HAMAP.
DR   HAMAP; MF_00021; ThiI; 1; -.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR003720; ThiI.
DR   InterPro; IPR020536; ThiI_C.
DR   InterPro; IPR004114; THUMP.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02568; ThiI; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   SMART; SM00981; THUMP; 1.
DR   TIGRFAMs; TIGR00342; TIGR00342; 1.
DR   PROSITE; PS51165; THUMP; 1.
DR   HOGENOMDNA; METP4_1.PE1013; -.
KW   ATP-binding; Complete proteome; Cytoplasm; Nucleotide-binding;
KW   RNA-binding; Thiamine biosynthesis; Transferase; tRNA-binding.
SQ   SEQUENCE   391 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MIRYGELFLK SEPVMRHYVG VLTANIKRAL DAEEICHEIT ITRGRIFVYG EDVDAINSVL
     LRIFGIVGTS IVICTPPDRE TIEKTAAIVA SRTLKPGMSF AVRARRSNIK GFTSQELAAS
     TGSCIYEAVG DLKVDLDNPE YEIFIEARSN GGFIYETRTP GPGGLPVGTQ GKVLCLLSAG
     IDSPVAAWLA MSRGCIPGFI YFDGGDYFGK DTNRAVLENL QNLSRWYPGS TVEAAFIDIT
     PFFDKLTDKS AKEALKNRCI ICKRFMLRLA GKCALDWKYL GLVTGNSIGQ VASQTLANIG
     ILGSAVPEGI ALIEPLITYD KEDTVKIARN IGTFRENAGD LSCGVVPSHP SIAATLAKVI
     EDEGMLGIPE LIEECIPRKT IYKAKDGKLL D
//

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