(data stored in ACNUC7421 zone)

HOGENOM: MYCTK_1_PE1004

ID   MYCTK_1_PE1004                       STANDARD;      PRT;   473 AA.
AC   MYCTK_1_PE1004; C6DW61;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1
DE   33;AltName: Full=Alpha-IPM isomerase;AltName: Full=Isopropylmalate
DE   isomerase; (MYCTK_1.PE1004).
GN   Name=leuC; OrderedLocusNames=TBMG_00981;
OS   MYCOBACTERIUM TUBERCULOSIS KZN 1435.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium
OC   tuberculosis complex.
OX   NCBI_TaxID=478434;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS MYCTK_1.PE1004.
CC       Mycobacterium tuberculosis KZN 1435, complete genome.
CC       chromosome, complete sequence.
CC   -!- ANNOTATIONS ORIGIN:C6DW61_MYCTK
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate
CC       and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-
CC       isopropylmaleate + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (2S)-2-isopropylmaleate + H(2)O = (2S)-2-
CC       isopropylmalate.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC       leucine from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD (By similarity).
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC
CC       type 1 subfamily.
CC   -!- GENE_FAMILY: HOG000226972 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; C6DW61; -.
DR   EMBL; CP001658; ACT24025.1; -; Genomic_DNA.
DR   RefSeq; YP_003030920.1; NC_012943.1.
DR   ProteinModelPortal; C6DW61; -.
DR   STRING; C6DW61; -.
DR   EnsemblBacteria; EBMYCT00000095622; EBMYCP00000087282; EBMYCG00000092312.
DR   GeneID; 8162274; -.
DR   GenomeReviews; CP001658_GR; TBMG_00981.
DR   KEGG; mtb:TBMG_00981; -.
DR   GeneTree; EBGT00050000014567; -.
DR   ProtClustDB; PRK05478; -.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_01026; LeuC_type1; 1; -.
DR   InterPro; IPR004430; 3-IsopropMal_deHydase_lsu.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR015937; Acoase/IPM_deHydtase.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR015936; Homoacnase/3IPM_deHydtase_su.
DR   Gene3D; G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2.
DR   Gene3D; G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1.
DR   PANTHER; PTHR11670; Aconitase-like_core; 1.
DR   PANTHER; PTHR11670:SF6; Homoacnase/3IPM_dehydase_s/lsu; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   SUPFAM; SSF53732; Aconitase_N; 1.
DR   TIGRFAMs; TIGR00170; LeuC; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
DR   HOGENOMDNA; MYCTK_1.PE1004; -.
KW   isopropylmalate isomerase large subunit;
KW   4Fe-4S; Amino-acid biosynthesis;
KW   Branched-chain amino acid biosynthesis; Complete proteome; Iron;
KW   Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding.
SQ   SEQUENCE   473 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MALQTGEPRT LAEKIWDDHI VVSGGGCAPD LIYIDLHLVH EVTSPQAFDG LRLAGRRVRR
     PELTLATEDH NVPTVDIDQP IADPVSRTQV ETLRRNCAEF GIRLHSMGDI EQGIVHVVGP
     QLGLTQPGMT IVCGDSHTST HGAFGALAMG IGTSEVEHVL ATQTLPLRPF KTMAVNVDGR
     LPDGVSAKDI ILALIAKIGT GGGQGHVIEY RGSAIESLSM EGRMTICNMS IEAGARAGMV
     APDETTYAFL RGRPHAPTGA QWDTALVYWQ RLRTDVGAVF DTEVYLDAAS LSPFVTWGTN
     PGQGVPLAAA VPDPQLMTDD AERQAAEKAL AYMDLRPGTA MRDIAVDAVF VGSCTNGRIE
     DLRVVAEVLR GRKVADGVRM LIVPGSMRVR AQAEAEGLGE IFTDAGAQWR QAGCSMCLGM
     NPDQLASGER CAATSNRNFE GRQGAGGRTH LVSPAVAAAT AVRGTLSSPA DLN
//

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