(data stored in ACNUC7421 zone)

HOGENOM: MYTUB1_1_PE2911

ID   MYTUB1_1_PE2911                      STANDARD;      PRT;   87 AA.
AC   MYTUB1_1_PE2911; O33348;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Toxin RelG; EC=3.1.- -;AltName: Full=Putative
DE   endoribonuclease RelG; (MYTUB1_1.PE2911).
GN   Name=relG; Synonyms=relE2; OrderedLocusNames=Rv2866;
OS   MYCOBACTERIUM TUBERCULOSIS H37RV.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium
OC   tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS MYTUB1_1.PE2911.
CC       Mycobacterium tuberculosis H37Rv, complete genome.
CC       1..69269 annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:RELG_MYCTU
CC   -!- FUNCTION: Toxic component of a toxin-antitoxin (TA) module. Has
CC       RNase activity and preferentially cleaves at the 3'-end of purine
CC       ribonucleotides (By similarity). Overexpression in M.tuberculosis
CC       or M.smegmatis inhibits colony formation in a bacteriostatic
CC       rather than bacteriocidal fashion. Its toxic effect is neutralized
CC       by coexpression with cognate antitoxin RelB2 (shown only for
CC       M.smegmatis). Overexpression also increases the number of
CC       gentamicin-tolerant and levofloxacin-tolerant persister cells.
CC   -!- FUNCTION: In combination with cognate antitoxin RelF represses its
CC       own promoter. Has been seen to bind DNA in complex with antitoxin
CC       RelF but not alone.
CC   -!- SUBUNIT: Interacts with cognate antitoxin RelF, which neutralizes
CC       the toxin. Also interacts with non-cognate antitoxin RelB in
CC       vitro, in M.smegmatis this neutralizes the toxicity of this toxin.
CC   -!- INDUCTION: Expressed in log phase cells. Induced by treatment with
CC       rifampicin and gentamicin as well as by oxidative, nitrosative and
CC       nutritional stress. Induced in the lungs of mice infected for 4
CC       weeks. A member of the relFG operon.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype in culture or upon
CC       infection of mice. Significantly fewer persister cells are
CC       generated in vitro following exposure to rifampicin and
CC       gentamicin, but in infected mice no differences are seen.
CC   -!- SIMILARITY: Belongs to the RelE toxin family.
CC   -!- GENE_FAMILY: HOG000219995 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; O33348; -.
DR   EMBL; BX842581; CAA15528.1; -; Genomic_DNA.
DR   PIR; D70886; D70886.
DR   RefSeq; NP_217382.1; NC_000962.2.
DR   PDB; 3G5O; X-ray; 2.00 A; B/C=2-87.
DR   PDBsum; 3G5O; -.
DR   ProteinModelPortal; O33348; -.
DR   EnsemblBacteria; EBMYCT00000003624; EBMYCP00000003624; EBMYCG00000003622.
DR   GeneID; 887450; -.
DR   GenomeReviews; AL123456_GR; Rv2866.
DR   KEGG; mtu:Rv2866; -.
DR   TubercuList; Rv2866; -.
DR   GeneTree; EBGT00050000018419; -.
DR   ProtClustDB; CLSK792131; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0015643; F:toxin binding; IDA:MTBBASE.
DR   GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR007712; Plasmid_stabil.
DR   Pfam; PF05016; Plasmid_stabil; 1.
DR   HOGENOMDNA; MYTUB1_1.PE2911; -.
KW   hypothetical protein;
KW   3D-structure; Complete proteome; DNA-binding; Hydrolase; Nuclease;
KW   Reference proteome; Repressor; Toxin; Transcription;
KW   Transcription regulation.
SQ   SEQUENCE   87 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MPYTVRFTTT ARRDLHKLPP RILAAVVEFA FGDLSREPLR VGKPLRRELA GTFSARRGTY
     RLLYRIDDEH TTVVILRVDH RADIYRR
//

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