(data stored in ACNUC9543 zone)

HOGENOM: OSTLU_10_PE112

ID   OSTLU_10_PE112                       STANDARD;      PRT;   284 AA.
AC   OSTLU_10_PE112; A4S8B3;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Diphthine synthase; EC=2.1.1 98; (OSTLU_10.PE112).
GN   ORFNames=OSTLU_40543;
OS   OSTREOCOCCUS LUCIMARINUS CCE9901.
OC   Eukaryota; Viridiplantae; Chlorophyta; Prasinophyceae; Mamiellales;
OC   Ostreococcus.
OX   NCBI_TaxID=436017;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS OSTLU_10.PE112.
CC       Ostreococcus lucimarinus (strain CCE9901) chromosome 15, complete
CC       sequence.
CC   -!- ANNOTATIONS ORIGIN:A4S8B3_OSTLU
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC       catalyzes the trimethylation of the amino group of the modified
CC       target histidine residue in translation elongation factor 2 (EF-
CC       2), to form an intermediate called diphthine. The three successive
CC       methylation reactions represent the second step of diphthamide
CC       biosynthesis (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3 S-adenosyl-L-methionine + 2-(3-carboxy-3-
CC       aminopropyl)-L-histidine = 3 S-adenosyl-L-homocysteine + 2-(3-
CC       carboxy-3-(trimethylammonio)propyl)-L-histidine.
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC   -!- SIMILARITY: Belongs to the diphthine synthase family.
CC   -!- GENE_FAMILY: HOG000205302 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; A4S8B3; -.
DR   EMBL; CP000595; ABO99885.1; -; Genomic_DNA.
DR   RefSeq; XP_001421592.1; XM_001421555.1.
DR   ProteinModelPortal; A4S8B3; -.
DR   STRING; A4S8B3; -.
DR   GeneID; 5005674; -.
DR   KEGG; olu:OSTLU_40543; -.
DR   OMA; TTHVDLR; -.
DR   ProtClustDB; CLSN2685890; -.
DR   GO; GO:0004164; F:diphthine synthase activity; IEA:EC.
DR   GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:InterPro.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR004551; Dphthn_synthase.
DR   Gene3D; G3DSA:3.40.1010.10; 4pyrrole_Mease_sub1; 1.
DR   Gene3D; G3DSA:3.30.950.10; 4pyrrole_Mease_sub2; 1.
DR   PANTHER; PTHR10882; Dphthn_synthase; 1.
DR   Pfam; PF00590; TP_methylase; 1.
DR   PIRSF; PIRSF036432; Diphthine_synth; 1.
DR   SUPFAM; SSF53790; Cor/por_Metransf; 1.
DR   TIGRFAMs; TIGR00522; Dph5; 1.
DR   HOGENOMDNA; OSTLU_10.PE112; -.
KW   ABO99885.11570-His-2caffold_90100000011;
KW   Predicted protein;
KW   Complete proteome; Methyltransferase; Transferase.
SQ   SEQUENCE   284 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MLYLIGLGLG NERDVTLRGL DAIKRCSKVY LEAYTSVLGV DVRAMEALYE KPVRTCDRAF
     VEQGVDAVLD EALSEDVAFC VVGDAFAATT HSDLVLRAIE RGVAVRPVYN ASIMNAVAGT
     GLSLYNFGKA VSICFFTQTW RPDSFYDLIR DNRKSGAHTL CLLDIRVKEP TVRALCKGIE
     EYEPARFMTA ATAAKQMLEV EESRGEGVYG EDTMCVAVAR IGQDDEKIRA CTLGEMRRVD
     MGAPLHSLVL VGDVMEIESA MLATHAVKPE DFYDGDAPCQ MLDG
//

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