(data stored in SCRATCH zone)

HOGENOM: PSEHT_1_PE1010

ID   PSEHT_1_PE1010                       STANDARD;      PRT;   469 AA.
AC   PSEHT_1_PE1010; Q3IK75;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
DE   EC=2.-.- -;AltName: Full=tRNA-i(6)A37 methylthiotransferase;
DE   (PSEHT_1.PE1010).
GN   Name=miaB; OrderedLocusNames=PSHAa1046;
OS   PSEUDOALTEROMONAS HALOPLANKTIS TAC125.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=326442;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS PSEHT_1.PE1010.
CC       Pseudoalteromonas haloplanktis TAC125 chromosome I, complete genome.
CC       sequence.
CC   -!- ANNOTATIONS ORIGIN:MIAB_PSEHT
CC   -!- FUNCTION: Catalyzes the methylthiolation of N6-
CC       (dimethylallyl)adenosine (i(6)A), leading to the formation of 2-
CC       methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37
CC       in tRNAs that read codons beginning with uridine (By similarity).
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters. One cluster is coordinated with
CC       3 cysteines and an exchangeable S-adenosyl-L-methionine (By
CC       similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 MTTase N-terminal domain.
CC   -!- SIMILARITY: Contains 1 TRAM domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAI86124.1; Type=Erroneous initiation;
CC   -!- GENE_FAMILY: HOG000224767 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; Q3IK75; -.
DR   EMBL; CR954246; CAI86124.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_339567.1; NC_007481.1.
DR   ProteinModelPortal; Q3IK75; -.
DR   STRING; Q3IK75; -.
DR   GeneID; 3710200; -.
DR   GenomeReviews; CR954246_GR; PSHAa1046.
DR   KEGG; pha:PSHAa1046; -.
DR   NMPDR; fig|326442.4.peg.899; -.
DR   eggNOG; COG0621; -.
DR   BioCyc; PHAL326442:PSHAA1046-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR   HAMAP; MF_01864; tRNA_metthiotr_MiaB; 1; -.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR023970; MeThioTfrase/B12-bd/rSAM.
DR   InterPro; IPR005839; Methylthiotransferase.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR013848; Methylthiotransferase_N.
DR   InterPro; IPR006463; MiaB_methiolase.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR002792; TRAM.
DR   Gene3D; G3DSA:3.80.30.20; G3DSA:3.80.30.20; 1.
DR   PANTHER; PTHR11918; UPF0004; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF00919; UPF0004; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR01574; MiaB-methiolase; 1.
DR   TIGRFAMs; TIGR00089; TIGR00089; 1.
DR   PROSITE; PS51449; MTTASE_N; 1.
DR   PROSITE; PS01278; MTTASE_RADICAL; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   HOGENOMDNA; PSEHT_1.PE1010; -.
KW   4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW   Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing.
SQ   SEQUENCE   469 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MNEYDSQKMA ELLDATNGYQ LTDDATDADV ILLNTCSIRE KAQEKVFHQL GRWKLLKDDK
     PDLIIGVGGC VASQEGDSIR QRAPFVDVIF GPQTLHRLPE MIKQVQGDKG SSVVDISFPE
     IEKFDRLPEP KADGPSAFVS IMEGCSKYCT FCVVPYTRGE EVSRPVDDVL LEIAQLAEQS
     VREVNLLGQN VNAYRGDTHD GEICYFSDLI RLIAAIDGID RIRYTTSHPV EFTQDIVDVY
     ADVPELVDHL HLPVQSGSDR ILNLMKRGHT AIEYKSTIRK LRKIRPNLSM SSDFIIGFPG
     ETQDDFEATM KLISDVGFDM SFSFIYSARP GTPAADLPDD VTEQEKKERL YLLQNRITQM
     AQQISRQMFD TEQRILVEGP SKKNPMELRG RTENNRVVNF VGPHTVIGQF VDVRITEALP
     NSLRGDLIRT ESEMNLRREI APSAILTKAA SLEPKPDTIN EIGVATFVP
//

If you have problems or comments...

PBIL Back to PBIL home page