(data stored in ACNUC27670 zone)


ID   RAT2_PE1283                          STANDARD;      PRT;   89 AA.
AC   RAT2_PE1283; P50115;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Protein S100-A8;AltName: Full=Calgranulin-A;AltName:
DE   Full=Migration inhibitory factor-related protein 8; Short=MRP-8;
DE   Short=p8;AltName: Full=S100 calcium-binding protein A8; (RAT2.PE1283).
GN   Name=S100a8; Synonyms=Mrp8;
OC   Eukaryota; Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia;
OC   Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi;
OC   Sarcopterygii; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;
OC   Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS RAT2.PE1283.
CC       Rattus norvegicus chromosome 2 RGSC3.4  sequence 1..258207540
CC       annotated by Ensembl
CC   -!- FUNCTION: Calcium-binding protein. Has antimicrobial activity
CC       towards bacteria and fungi. Important for resistance to invasion
CC       by pathogenic bacteria. Up-regulates transcription of genes that
CC       are under the control of NF-kappa-B. Plays a role in the
CC       development of endotoxic shock in response to bacterial
CC       lipopolysaccharide (LPS) (By similarity). Promotes tubulin
CC       polymerization. Promotes phagocyte migration and infiltration of
CC       granulocytes at sites of wounding. Plays a role as pro-
CC       inflammatory mediator in acute and chronic inflammation and up-
CC       regulates the release of IL8 and cell-surface expression of ICAM1.
CC       Extracellular calprotectin binds to target cells and promotes
CC       apoptosis. Antimicrobial and proapoptotic activity is inhibited by
CC       zinc ions (By similarity). Has maximal chemotactic activity for
CC       neutrophils. May play an important role in cellular immune
CC       reactions.
CC   -!- SUBUNIT: Homodimer. Heterotetramer with S100A9. Component of the
CC       heterotetrameric calprotectin complex containing two copies each
CC       of S100A8 and S100A9. Interacts with AGER and with the
CC       heterodimeric complex formed by TLR4 and LY96. Interacts with
CC       CEACAM3 and tubulin filaments in a calcium-dependent manner.
CC       Heterotetrameric calprotectin interacts with ANXA6 and associates
CC       with tubulin filaments in activated monocytes. May interact with
CC       components of the intermediate filaments in monocytes and
CC       epithelial cells (By similarity).
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity). Cytoplasm (By
CC       similarity). Cytoplasm, cytoskeleton (By similarity). Cell
CC       membrane; Peripheral membrane protein (By similarity).
CC       Note=Associates with tubulin filaments in activated monocytes.
CC       Targeted to the cell surface upon calcium influx. Released from
CC       blood leukocytes upon exposure to CSF2/GM-CSF, bacterial
CC       lipopolysaccharide (LPS) and during inflammatory processes.
CC   -!- MASS SPECTROMETRY: Mass=10149; Mass_error=2; Method=Electrospray;
CC       Range=2-89; Source=PubMed:9570842;
CC   -!- MISCELLANEOUS: Binds two calcium ions per molecule with an
CC       affinity similar to that of the S100 proteins (By similarity).
CC   -!- SIMILARITY: Belongs to the S-100 family.
CC   -!- SIMILARITY: Contains 2 EF-hand domains.
CC   -!- GENE_FAMILY: HOG000246968 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Rattus_norvegicus;ENSRNOG00000011557;ENSRNOT00000015473;ENSRNOP00000015473.
DR   EMBL; L18891; - ;
DR   UniProtKB/Swiss-Prot; P50115; -.
DR   EMBL; L18891; AAA41637.1; -; mRNA.
DR   IPI; IPI00231370; -.
DR   PIR; JN0685; JN0685.
DR   RefSeq; NP_446274.2; NM_053822.2.
DR   UniGene; Rn.31839; -.
DR   ProteinModelPortal; P50115; -.
DR   SMR; P50115; 1-89.
DR   STRING; P50115; -.
DR   PRIDE; P50115; -.
DR   Ensembl; ENSRNOT00000015473; ENSRNOP00000015473; ENSRNOG00000011557.
DR   GeneID; 116547; -.
DR   KEGG; rno:116547; -.
DR   UCSC; NM_053822; rat.
DR   CTD; 6279; -.
DR   RGD; 620265; S100a8.
DR   eggNOG; roNOG17670; -.
DR   GeneTree; ENSGT00590000083207; -.
DR   InParanoid; P50115; -.
DR   OrthoDB; EOG4V9TS7; -.
DR   PhylomeDB; P50115; -.
DR   NextBio; 619165; -.
DR   ArrayExpress; P50115; -.
DR   Genevestigator; P50115; -.
DR   GermOnline; ENSRNOG00000011557; Rattus norvegicus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005615; C:extracellular space; IDA:RGD.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0002526; P:acute inflammatory response; IEP:RGD.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR   GO; GO:0002544; P:chronic inflammatory response; IEP:RGD.
DR   GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR   GO; GO:0010043; P:response to zinc ion; IEP:RGD.
DR   GO; GO:0042060; P:wound healing; IEP:RGD.
DR   InterPro; IPR011992; EF-hand-like_dom.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR018249; EF_HAND_2.
DR   InterPro; IPR001751; S100/CaBP-9k_CS.
DR   InterPro; IPR013787; S100_Ca-bd_sub.
DR   Gene3D; G3DSA:; EF-Hand_type; 1.
DR   Pfam; PF01023; S_100; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
DR   PROSITE; PS00303; S100_CABP; 1.
KW   ENSRNOG00000011557820036002503210000011;
KW   S100a8; S10A8_RAT; L18891;
KW   Acetylation; Antimicrobial; Calcium; Cell membrane; Chemotaxis;
KW   Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW   Membrane; Reference proteome; Repeat; Secreted.

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