(data stored in ACNUC3759 zone)

HOGENOM: RAT4_PE1180

ID   RAT4_PE1180                          STANDARD;      PRT;   262 AA.
AC   RAT4_PE1180; P18297;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Sepiapterin reductase; Short=SPR; EC=1.1.1 153;
DE   (RAT4.PE1180).
GN   Name=Spr;
OS   RATTUS NORVEGICUS.
OC   Eukaryota; Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia;
OC   Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi;
OC   Sarcopterygii; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;
OC   Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS RAT4.PE1180.
CC       Rattus norvegicus chromosome 4 RGSC3.4  sequence 1..187126005
CC       annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:SPRE_RAT
CC   -!- FUNCTION: Catalyzes the final one or two reductions in tetra-
CC       hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
CC   -!- CATALYTIC ACTIVITY: 7,8-dihydrobiopterin + NADP(+) = sepiapterin +
CC       NADPH.
CC   -!- CATALYTIC ACTIVITY: Tetrahydrobiopterin + 2 NADP(+) = 6-pyruvoyl-
CC       5,6,7,8-tetrahydropterin + 2 NADPH.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=12.6 uM for sepiapterin;
CC         KM=2.8 uM for NADPH;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: In vitro phosphorylation of Ser-46, Ser-196 and Ser-214 by
CC       CaMK2 does not change kinetic parameters.
CC   -!- SIMILARITY: Belongs to the sepiapterin reductase family.
CC   -!- GENE_FAMILY: HOG20000000 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Rattus_norvegicus;ENSRNOG00000015455;ENSRNOT00000020749;ENSRNOP00000020749.
DR   EMBL; BC166809; - ;
DR   EMBL; CH473957; - ;
DR   EMBL; M36410; - ;
DR   UniProtKB/Swiss-Prot; P18297; -.
DR   EMBL; M36410; AAA42130.1; -; mRNA.
DR   IPI; IPI00189989; -.
DR   PIR; A36024; A36024.
DR   RefSeq; NP_062054.1; NM_019181.1.
DR   UniGene; Rn.6658; -.
DR   ProteinModelPortal; P18297; -.
DR   SMR; P18297; 6-261.
DR   STRING; P18297; -.
DR   PhosphoSite; P18297; -.
DR   PRIDE; P18297; -.
DR   Ensembl; ENSRNOT00000020749; ENSRNOP00000020749; ENSRNOG00000015455.
DR   GeneID; 29270; -.
DR   KEGG; rno:29270; -.
DR   CTD; 6697; -.
DR   RGD; 3753; Spr.
DR   eggNOG; roNOG10314; -.
DR   GeneTree; ENSGT00440000033609; -.
DR   InParanoid; P18297; -.
DR   OMA; NISSVCA; -.
DR   OrthoDB; EOG4HMJB9; -.
DR   PhylomeDB; P18297; -.
DR   NextBio; 608616; -.
DR   ArrayExpress; P18297; -.
DR   Genevestigator; P18297; -.
DR   GermOnline; ENSRNOG00000015455; Rattus norvegicus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004757; F:sepiapterin reductase activity; IDA:UniProtKB.
DR   GO; GO:0010033; P:response to organic substance; IMP:RGD.
DR   GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IMP:RGD.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006393; Sepiapterin_red.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   TIGRFAMs; TIGR01500; Sepiapter_red; 1.
DR   HOGENOMDNA; RAT4.PE1180; -.
KW   ENSRNOG00000015455820036002503210000011;
KW   Spr; SPRE_RAT; B2RYK3_RAT; BC166809; CH473957; M36410;
KW   Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing;
KW   NADP; Oxidoreductase; Phosphoprotein; Reference proteome.
SQ   SEQUENCE   262 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MEGGRLGCAV CVLTGASRGF GRALAPQLAG LLSPGSVLLL SARSDSMLRQ LKEELCTQQP
     GLQVVLAAAD LGTESGVQQL LSAVRELPRP ERLQRLLLIN NAGTLGDVSK GFLNINDLAE
     VNNYWALNLT SMLCLTTGTL NAFSNSPGLS KTVVNISSLC ALQPFKGWGL YCAGKAARDM
     LYQVLAVEEP SVRVLSYAPG PLDTNMQQLA RETSMDPELR SRLQKLNSEG ELVDCGTSAQ
     KLLSLLQRDT FQSGAHVDFY DI
//

If you have problems or comments...

PBIL Back to PBIL home page