(data stored in ACNUC5835 zone)

HOVERGEN: SCF_RAT

ID   SCF_RAT                 Reviewed;         273 AA.
AC   P21581; Q9QWZ4; Q9Z2E7;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   13-OCT-2009, entry version 78.
DE   RecName: Full=Kit ligand;
DE   AltName: Full=C-kit ligand;
DE   AltName: Full=Stem cell factor;
DE            Short=SCF;
DE   AltName: Full=Mast cell growth factor;
DE            Short=MGF;
DE   AltName: Full=Hematopoietic growth factor KL;
DE   Flags: Precursor;
GN   Name=Kitlg; Synonyms=Kitl, Mgf;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA   Teramoto T., Nagashima M., Thorgeirsson S.S.;
RL   Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-201, AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=91004219; PubMed=2208279; DOI=10.1016/0092-8674(90)90301-T;
RA   Martin F.H., Suggs S.V., Langley K.E., Lu H.S., Ting J., Okino K.H.,
RA   Morris C.F., McNiece I.K., Jacobsen F.W., Mendiaz E.A., Birkett N.C.,
RA   Smith K.A., Johnson M.J., Parker V.P., Flores J.C., Patel A.C.,
RA   Fisher E.F., Erjavec H.O., Herrera C.J., Wypych J., Sachdev R.K.,
RA   Pope J.A., Leslie I., Wen D., Lin C.-H., Cupples R.L., Zsebo K.M.;
RT   "Primary structure and functional expression of rat and human stem
RT   cell factor DNAs.";
RL   Cell 63:203-211(1990).
RN   [3]
RP   PROTEIN SEQUENCE OF 26-190, PYROGLUTAMATE FORMATION AT GLN-26,
RP   GLYCOSYLATION AT ASN-90; ASN-134; ASN-145; SER-167; THR-168; THR-180
RP   AND ASN-195, AND DISULFIDE BONDS.
RC   STRAIN=Buffalo; TISSUE=Liver;
RX   MEDLINE=91217037; PubMed=1708771;
RA   Lu H.S., Clogston C.L., Wypych J., Fausset P.R., Lauren S.,
RA   Mendiaz E.A., Zsebo K.M., Langley K.E.;
RT   "Amino acid sequence and post-translational modification of stem cell
RT   factor isolated from buffalo rat liver cell-conditioned medium.";
RL   J. Biol. Chem. 266:8102-8107(1991).
RN   [4]
RP   PROTEIN SEQUENCE OF 26-39.
RX   MEDLINE=91004218; PubMed=2208278; DOI=10.1016/0092-8674(90)90300-4;
RA   Zsebo K.M., Wypych J., McNiece I.K., Lu H.S., Smith K.A.,
RA   Karkare S.B., Sachdev R.K., Yuschenkoff V.N., Birkett N.C.,
RA   Williams L.R., Satyagal V.N., Tung W., Bosselman R.A., Mendiaz E.A.,
RA   Langley K.E.;
RT   "Identification, purification, and biological characterization of
RT   hematopoietic stem cell factor from buffalo rat liver-conditioned
RT   medium.";
RL   Cell 63:195-201(1990).
CC   -!- FUNCTION: Stimulates the proliferation of mast cells. Able to
CC       augment the proliferation of both myeloid and lymphoid
CC       hematopoietic progenitors in bone marrow culture. Mediates also
CC       cell-cell adhesion. Acts synergistically with other cytokines,
CC       probably interleukins.
CC   -!- SUBUNIT: Homodimer, non-covalently linked (Probable).
CC   -!- SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I
CC       membrane protein (By similarity). Secreted (By similarity).
CC       Note=Also exists as a secreted soluble form (isoform 1 only) (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Single-pass type I
CC       membrane protein (By similarity). Cytoplasm, cytoskeleton (By
CC       similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=KL-1;
CC         IsoId=P21581-1; Sequence=Displayed;
CC       Name=2; Synonyms=KL-2;
CC         IsoId=P21581-2; Sequence=VSP_006025;
CC   -!- DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis.
CC   -!- PTM: A soluble form is produced by proteolytic processing of
CC       isoform 1 in the extracellular domain.
CC   -!- PTM: The identity of N- and O-linked saccharides are not reported
CC       in PubMed:1708771. The O-linked polysaccharides are probably the
CC       mucin type linked to GalNAc.
CC   -!- SIMILARITY: Belongs to the SCF family.
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CC   -!- GENE_FAMILY: HBG036146 [ FAMILY / ALN / TREE ]
DR   EMBL; AF071204; AAD02827.1; -; mRNA.
DR   EMBL; AF071205; AAD02828.1; -; mRNA.
DR   EMBL; M59966; AAA42117.1; -; mRNA.
DR   IPI; IPI00204342; -.
DR   IPI; IPI00231296; -.
DR   PIR; B35974; B35974.
DR   RefSeq; NP_068615.1; -.
DR   UniGene; Rn.44216; -.
DR   SMR; P21581; 28-166.
DR   STRING; P21581; -.
DR   Ensembl; ENSRNOT00000007231; ENSRNOP00000007231; ENSRNOG00000005386; Rattus norvegicus.
DR   Ensembl; ENSRNOT00000058921; ENSRNOP00000055705; ENSRNOG00000005386; Rattus norvegicus.
DR   GeneID; 60427; -.
DR   KEGG; rno:60427; -.
DR   UCSC; NM_021843; rat.
DR   CTD; 60427; -.
DR   RGD; 708536; Kitl.
DR   HOVERGEN; P21581; -.
DR   NextBio; 612146; -.
DR   ArrayExpress; P21581; -.
DR   Genevestigator; P21581; -.
DR   GermOnline; ENSRNOG00000005386; Rattus norvegicus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0005173; F:stem cell factor receptor binding; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   InterPro; IPR003452; SCF.
DR   PANTHER; PTHR11574; SCF; 1.
DR   Pfam; PF02404; SCF; 1.
DR   PIRSF; PIRSF015599; SCF; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P21581.
DR   SWISS-2DPAGE; P21581.
KW   Alternative splicing; Cell adhesion; Cell membrane; Cytoplasm;
KW   Cytoskeleton; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Growth factor; Membrane; Pyrrolidone carboxylic acid; Secreted;
KW   Signal; Transmembrane.
FT   DOMAIN        1    121       PRODOM:2005.1:PD005657  20
FT   DOMAIN      122    207       PRODOM:2005.1:PD006155  15
FT   DOMAIN      210    273       PRODOM:2005.1:PD981191  13
FT   SIGNAL        1     25
FT   CHAIN        26    273       Kit ligand.
FT                                /FTId=PRO_0000031917.
FT   TOPO_DOM     26    214       Extracellular (Potential).
FT   TRANSMEM    215    237       Potential.
FT   TOPO_DOM    238    273       Cytoplasmic (Potential).
FT   SITE         97     97       Not glycosylated.
FT   MOD_RES      26     26       Pyrrolidone carboxylic acid.
FT   CARBOHYD     90     90       N-linked (GlcNAc...); partial.
FT   CARBOHYD    134    134       N-linked (GlcNAc...); partial.
FT   CARBOHYD    145    145       N-linked (GlcNAc...).
FT   CARBOHYD    167    167       O-linked (GalNAc...) (Probable).
FT   CARBOHYD    168    168       O-linked (GalNAc...) (Probable).
FT   CARBOHYD    180    180       O-linked (GalNAc...) (Probable).
FT   CARBOHYD    195    195       N-linked (GlcNAc...) (Potential).
FT   DISULFID     29    114
FT   DISULFID     68    163
FT   VAR_SEQ     174    202       DSRVSVTKPFMLPPVAASSLRNDSSSSNR -> G (in
FT                                isoform 2).
FT                                /FTId=VSP_006025.
FT   CONFLICT    207    207       S -> P (in Ref. 1; AAD02828).
SQ   SEQUENCE   273 AA;  30712 MW;  C0F56527DC93FD27 CRC64;
     MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND YMITLNYVAG
     MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP
     KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT
     KPFMLPPVAA SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK
     QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
//

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