(data stored in SCRATCH zone)

HOGENOM: SHESM_1_PE1002

ID   SHESM_1_PE1002                       STANDARD;      PRT;   474 AA.
AC   SHESM_1_PE1002; Q0HLI3;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
DE   EC=2.-.- -;AltName: Full=tRNA-i(6)A37 methylthiotransferase;
DE   (SHESM_1.PE1002).
GN   Name=miaB; OrderedLocusNames=Shewmr4_1004;
OS   SHEWANELLA SP. MR-4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=60480;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS SHESM_1.PE1002.
CC       Shewanella sp. MR-4, complete genome.
CC       annotated by Ensembl Genomes
CC   -!- ANNOTATIONS ORIGIN:MIAB_SHESM
CC   -!- FUNCTION: Catalyzes the methylthiolation of N6-
CC       (dimethylallyl)adenosine (i(6)A), leading to the formation of 2-
CC       methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37
CC       in tRNAs that read codons beginning with uridine (By similarity).
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters. One cluster is coordinated with
CC       3 cysteines and an exchangeable S-adenosyl-L-methionine (By
CC       similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 MTTase N-terminal domain.
CC   -!- SIMILARITY: Contains 1 TRAM domain.
CC   -!- GENE_FAMILY: HOG000224767 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; Q0HLI3; -.
DR   EMBL; CP000446; ABI38084.1; -; Genomic_DNA.
DR   RefSeq; YP_733141.1; NC_008321.1.
DR   ProteinModelPortal; Q0HLI3; -.
DR   STRING; Q0HLI3; -.
DR   GeneID; 4251042; -.
DR   GenomeReviews; CP000446_GR; Shewmr4_1004.
DR   KEGG; she:Shewmr4_1004; -.
DR   eggNOG; COG0621; -.
DR   OMA; DSAYTFI; -.
DR   ProtClustDB; PRK14325; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR   HAMAP; MF_01864; tRNA_metthiotr_MiaB; 1; -.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR023970; MeThioTfrase/B12-bd/rSAM.
DR   InterPro; IPR005839; Methylthiotransferase.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR013848; Methylthiotransferase_N.
DR   InterPro; IPR006463; MiaB_methiolase.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR002792; TRAM.
DR   Gene3D; G3DSA:3.80.30.20; G3DSA:3.80.30.20; 1.
DR   PANTHER; PTHR11918; UPF0004; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF00919; UPF0004; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR01574; MiaB-methiolase; 1.
DR   TIGRFAMs; TIGR00089; TIGR00089; 1.
DR   PROSITE; PS51449; MTTASE_N; 1.
DR   PROSITE; PS01278; MTTASE_RADICAL; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   HOGENOMDNA; SHESM_1.PE1002; -.
KW   4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW   Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing.
SQ   SEQUENCE   474 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MSKKLHIKTW GCQMNEYDSS KMADLLGEYQ GYTLTEEAEE ADILLLNTCS IREKAQEKVF
     HQLGRWKTLK DKNPDLIIGV GGCVASQEGK AIKDRAHCVD IIFGPQTLHR LPDMIEQVRR
     GEKAVIDVSF PEIEKFDRLP EPRAEGPTAF VSIMEGCSKY CSFCVVPYTR GEEVSRPSDD
     IILEIAQLAE QGVREVNLLG QNVNAYRGAT HDGGICTFAE LLRYVAAIDG IDRIRFTTSH
     PIEFTQDIID VYEDTPELVS FLHLPVQSGS DRILTAMKRG HMAIEYKSII RRLRKARPDI
     QISSDFIIGF PGETKEDFAD TMKLIEDVAF DHSFSFIYSA RPGTPAADLP DDVDMEEKKQ
     RLAILQDRIT QQAMRYSRHM MGTVQRILVE GPSVKNPMEL RGRTENNRVV NFEGLPKHIG
     SFVDVEIVDV YTNSLRGKFI RGEDEMDLRR NLRPSDILAK HKQDDDLGVT QFKP
//

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